Prototype of arachidonic acid production from mortierella alpina: separation, purification, and characterization / (Record no. 177320)
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| 000 -LEADER | |
|---|---|
| fixed length control field | 04671namaa22004451i 4500 |
| 003 - CONTROL NUMBER IDENTIFIER | |
| control field | EG-GICUC |
| 005 - أخر تعامل مع التسجيلة | |
| control field | 20260103143310.0 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION | |
| fixed length control field | 260103s2025 ua a|||frm||| 000 0 eng d |
| 040 ## - CATALOGING SOURCE | |
| Original cataloguing agency | EG-GICUC |
| Language of cataloging | eng |
| Transcribing agency | EG-GICUC |
| Modifying agency | EG-GICUC |
| Description conventions | rda |
| 041 0# - LANGUAGE CODE | |
| Language code of text/sound track or separate title | eng |
| Language code of summary or abstract | eng |
| -- | ara |
| 049 ## - Acquisition Source | |
| Acquisition Source | Deposit |
| 082 04 - DEWEY DECIMAL CLASSIFICATION NUMBER | |
| Classification number | 660.6 |
| 092 ## - LOCALLY ASSIGNED DEWEY CALL NUMBER (OCLC) | |
| Classification number | 660.6 |
| Edition number | 21 |
| 097 ## - Degree | |
| Degree | Ph.D |
| 099 ## - LOCAL FREE-TEXT CALL NUMBER (OCLC) | |
| Local Call Number | Cai01.12.25.Ph.D.2025.Vi.P |
| 100 0# - MAIN ENTRY--PERSONAL NAME | |
| Authority record control number or standard number | Violette Said Hanna Youssef, |
| Preparation | preparation. |
| 245 10 - TITLE STATEMENT | |
| Title | Prototype of arachidonic acid production from mortierella alpina: separation, purification, and characterization / |
| Statement of responsibility, etc. | by Violette Said Hanna Youssef ; Supervisors Prof. Dr. Tahany Mohamed Abdel Rahman, Dr. Mohamed Naguib Abd El-Ghany, Dr. Hatem Abdel Moneim Tallima. |
| 246 15 - VARYING FORM OF TITLE | |
| Title proper/short title | نموذج أولي لإنتاج حمض الاراكيدونيك من النوع الفطري :Mortierella alpina فصل، تنقية، و توصيف |
| 264 #0 - PRODUCTION, PUBLICATION, DISTRIBUTION, MANUFACTURE, AND COPYRIGHT NOTICE | |
| Date of production, publication, distribution, manufacture, or copyright notice | 2025. |
| 300 ## - PHYSICAL DESCRIPTION | |
| Extent | 133 pages : |
| Other physical details | illustrations ; |
| Dimensions | 25 cm. + |
| Accompanying material | CD. |
| 336 ## - CONTENT TYPE | |
| Content type term | text |
| Source | rda content |
| 337 ## - MEDIA TYPE | |
| Media type term | Unmediated |
| Source | rdamedia |
| 338 ## - CARRIER TYPE | |
| Carrier type term | volume |
| Source | rdacarrier |
| 502 ## - DISSERTATION NOTE | |
| Dissertation note | Thesis (Ph.D)-Cairo University, 2025. |
| 504 ## - BIBLIOGRAPHY, ETC. NOTE | |
| Bibliography, etc. note | Bibliography: pages 108-130. |
| 520 #3 - SUMMARY, ETC. | |
| Summary, etc. | Arachidonic acid (ARA) is primordial for the proper functioning of human body, through maintaining the integrity of nervous, muscular, and immune systems functions. Production of this essential fatty acid can be achieved by the oleaginous fungus, Mortierella alpina. The identification of M. alpina, which was sourced from Assiut University, Egypt, was confirmed via the sequencing of the ITS region which is considered as DNA barcoding and the mRNA of the elongation enzyme. Culture conditions, including the use of glucose and peptone in liquid media as carbon and nitrogen sources, along with various micronutrients, were optimized to improve biomass and ARA production. Shake flask cultivation at 25°C for 7 days yielded approximately 0.570 g of ARA per liter of culture. Treatment of Mortierella alpina with the mutagen 5-fluorouracil, along with screening using octyl gallate-supplemented glucose-yeast-agar plates, followed by incubation at 25°C, then 20°C, and aging at 10°C, extraction following the Folch method, and isolation via urea exclusion resulted in a yield exceeding 3 g of ARA per liter of culture, indicating its potential suitability for commercial production and schistosome control. |
| 520 #3 - SUMMARY, ETC. | |
| Summary, etc. | يعتبر حمض الأراكيدونيك (ARA) أساسيًا للحفاظ على الجهاز العصبي والعضلي والمناعي. يمكن إنتاج هذا الحمض الدهني الأساسي عن طريق الفطر الزيتي Mortierella alpina. تم التأكد من M. alpina - والذي تم الحصول عليه من جامعة أسيوط، مصر- من خلال تسلسل منطقة (ITS) التي تعتبر بمثابة ترميز (DNA) وتسلسل (mRNA) لإنزيم الاستطالة. تم تحسين ظروف الزراعة الفطرية، عن طريق استخدام الجلوكوز والببتون كمصادر للكربون والنيتروجين، إلى جانب المغذيات الدقيقة المختلفة، لتحسين إنتاج الكتلة الحيوية والـ (ARA). أنتجت الزراعة الفطرية عند درجة حرارة 25 درجة مئوية لمدة 7 أيام حوالي 0.570 جرام من ((ARA لكل لتر من المزرعة. حدوث طفرة ل (Mortierella alpina) باستخدام 5-فلورويوراسيل، وباستخدام الجلوكوز والخميرة المكملة بأوكتيل جالات، يليها الحضانة عند 25 درجة مئوية، ثم 20 درجة مئوية، وعند 10 درجات مئوية، ادي إلى إنتاج يتجاوز 3 جرام من (ARA) لكل لتر من المزرعة، مما يشير إلى ملاءمتها للإنتاج التجاري. |
| 530 ## - ADDITIONAL PHYSICAL FORM AVAILABLE NOTE | |
| Issues CD | Issues also as CD. |
| 546 ## - LANGUAGE NOTE | |
| Text Language | Text in English and abstract in Arabic & English. |
| 650 #0 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | Biotechnology |
| 650 #0 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | اﻟﺘﻜﻨﻮﻟﻮﺟﯿﺎ اﻟﺤﯿﻮﯾا |
| 653 #0 - INDEX TERM--UNCONTROLLED | |
| Uncontrolled term | Mortierella alpina |
| -- | Arachidonic acid |
| -- | GLELO |
| -- | PUFA |
| -- | 5-fluorouracil |
| 700 0# - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Tahany Mohamed Abdel Rahman |
| Relator term | thesis advisor. |
| 700 0# - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Mohamed Naguib Abd El-Ghany |
| Relator term | thesis advisor. |
| 700 0# - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Hatem Abdel Moneim Tallima |
| Relator term | thesis advisor. |
| 900 ## - Thesis Information | |
| Grant date | 01-01-2025 |
| Supervisory body | Tahany Mohamed Abdel Rahman |
| -- | Mohamed Naguib Abd El-Ghany |
| -- | Hatem Abdel Moneim Tallima |
| Universities | Cairo University |
| Faculties | Faculty of Biotechnology |
| Department | Department of Biotechnology |
| 905 ## - Cataloger and Reviser Names | |
| Cataloger Name | Shimaa |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) | |
| Source of classification or shelving scheme | Dewey Decimal Classification |
| Koha item type | Thesis |
| Edition | 21 |
| Suppress in OPAC | No |
| Source of classification or shelving scheme | Home library | Current library | Date acquired | Inventory number | Full call number | Barcode | Date last seen | Effective from | Koha item type |
|---|---|---|---|---|---|---|---|---|---|
| Dewey Decimal Classification | المكتبة المركزبة الجديدة - جامعة القاهرة | قاعة الرسائل الجامعية - الدور الاول | 03.01.2026 | 93034 | Cai01.12.25.Ph.D.2025.Vi.P | 01010110093034000 | 03.01.2026 | 03.01.2026 | Thesis |