Laser assisted ALU-qPCR to evaluate the association between cell-free dna in human follicular fluid with oocyte and embryo quality in assisted reproductive techniques / (رقم التسجيلة. 179579)
[ عرض عادي ]
| 000 -LEADER | |
|---|---|
| fixed length control field | 05879namaa22004571i 4500 |
| 003 - CONTROL NUMBER IDENTIFIER | |
| control field | EG-GICUC |
| 005 - أخر تعامل مع التسجيلة | |
| control field | 20260419133655.0 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION | |
| fixed length control field | 260419s2025 ua a|||frm||| 000 0 eng d |
| 040 ## - CATALOGING SOURCE | |
| Original cataloguing agency | EG-GICUC |
| Language of cataloging | eng |
| Transcribing agency | EG-GICUC |
| Modifying agency | EG-GICUC |
| Description conventions | rda |
| 041 0# - LANGUAGE CODE | |
| Language code of text/sound track or separate title | eng |
| Language code of summary or abstract | eng |
| -- | ara |
| 049 ## - Acquisition Source | |
| Acquisition Source | Deposit |
| 082 04 - DEWEY DECIMAL CLASSIFICATION NUMBER | |
| Classification number | 621.366 |
| 092 ## - LOCALLY ASSIGNED DEWEY CALL NUMBER (OCLC) | |
| Classification number | 621.366 |
| Edition number | 21 |
| 097 ## - Degree | |
| Degree | Ph.D |
| 099 ## - LOCAL FREE-TEXT CALL NUMBER (OCLC) | |
| Local Call Number | Cai01.24.03.Ph.D.2025.Ra.L |
| 100 0# - MAIN ENTRY--PERSONAL NAME | |
| Authority record control number or standard number | Rabab Mohamed Abdel Hakim Sorour, |
| Preparation | preparation. |
| 245 10 - TITLE STATEMENT | |
| Title | Laser assisted ALU-qPCR to evaluate the association between cell-free dna in human follicular fluid with oocyte and embryo quality in assisted reproductive techniques / |
| Statement of responsibility, etc. | by Rabab Mohamed Abdel Hakim Sorour ; Supervision Prof. Mohamed Amr Hussien El-Noury, Prof. Osama Fekry Ahmed Al-Balah, Prof. Tamer Fouad Taha, Dr. Sondos Mohamed Mohamed Salem. |
| 246 15 - VARYING FORM OF TITLE | |
| Title proper/short title | تفاعل البلمرة المتسلسل بمساعة الليزر لتقييم العلاقة بين الحمض النووي الحر في السائل حول البويضة بجودة البويضة والجنين أثناء استخدام التقنيات المساعدة علي الإنجاب |
| 264 #0 - PRODUCTION, PUBLICATION, DISTRIBUTION, MANUFACTURE, AND COPYRIGHT NOTICE | |
| Date of production, publication, distribution, manufacture, or copyright notice | 2025. |
| 300 ## - PHYSICAL DESCRIPTION | |
| Extent | 111 pages : |
| Other physical details | illustrations ; |
| Dimensions | 25 cm. + |
| Accompanying material | CD. |
| 336 ## - CONTENT TYPE | |
| Content type term | text |
| Source | rda content |
| 337 ## - MEDIA TYPE | |
| Media type term | Unmediated |
| Source | rdamedia |
| 338 ## - CARRIER TYPE | |
| Carrier type term | volume |
| Source | rdacarrier |
| 502 ## - DISSERTATION NOTE | |
| Dissertation note | Thesis (Ph.D)-Cairo University, 2025. |
| 504 ## - BIBLIOGRAPHY, ETC. NOTE | |
| Bibliography, etc. note | Bibliography: pages 108-111. |
| 520 #3 - SUMMARY, ETC. | |
| Summary, etc. | Background: Cell-free DNA (cfDNA) has the potential to provide non- invasive diagnostic assays and practical biomarkers for predicting the outcome of assisted reproductive technology (ART) procedures. Aim of the study: To investigate the association between cf-DNA concentration in individual follicular fluid and oocyte maturity and embryo quality in patients undergoing Intra Cytoplasmic Sperm injection (ICSI).<br/>Patients and met hods: This prospective observational clinical study was done at Ain Shams University, A maternity hospital (IVF Unit), and a private ICSI center from May 2019 to June 2020. Our study used the ALU- QPCR technique to accurately measure the cell-free DNA (cfDNA) level in 35 follicular fluid samples from 15 patients undergoing ICSI.<br/>Results: Our results displayed the range of cfDNA from 0.24 to 3.89 (ng/ ml), with a mean of 2.06 ± 0.73. The DNA integrity (Q247/Q115) ranged from 0.09 to 0.79, with a mean of 0.44 ± 0.18. Our findings reveal a statistically significant association between the levels of cell-free DNA and patient oocytes to yield an Embryo, top grade Embryos, Fragmentation rate of the embryo, and number of blastomeres (P-values; p=0.037, p<0.001, p<0.001, and p=0.019, respectively ). A statistically negative correlation between cfDNA (ng/ml) and follicle size in mm (p-value < 0.05). However, no significant correlation was observed for the other parameters (basic hormonal profile, AFC number of oocytes, and the number of fertilized oocytes), with a p-value > 0.05. The study found that the levels of cfDNA and 17 -estradiol had a non-significant negative correlation (p-value > 0.05). Our study found that the level of cfDNA in follicular fluid is strongly associated with the quality of embryos on Day 3.<br/>Conclusion: High levels of cfDNA in follicular fluid may indicate poor follicular milieu. Measuring cfDNA can evaluate oocyte and embryo competence. It can be a non-invasive test to assess embryo quality along with morphological criteria. |
| 520 #3 - SUMMARY, ETC. | |
| Summary, etc. | عرضت نتائجنا نطاق cfDNA من 0.24 إلى 3.89 (نانوغرام/ مل)، بمتوسط 2.06 ± 0.73. تراوحت سلامة الحمض النووي (Q247/Q115) من 0.09 إلى 0.79، بمتوسط 0.44 ± 0.18. تكشف النتائج التي توصلنا إليها عن وجود علاقة ذات دلالة إحصائية بين مستويات الحمض النووي الحر خارج الخلايا (cfDNA) مع بويضات المريضات التى أجنة والأجنة من الدرجة الأولى ومعدل التجزئة الجنين وعدد القسيمات المنقسمة (القيم P؛ p=0.037، p<0.001، ع <0.001، و ع = 0.019، على التوالي). هناك علاقة سلبية إحصائيا بين الحمض النووي الحر خارج الخلايا (cfDNA) (نانوغرام / مل) وحجم الحويصلات بالملليمتر (القيمة p <0.05). ومع ذلك، لم يلاحظ أي ارتباط كبير بالنسبة للمعايير الأخرى (الملف الهرموني الأساسي، وعدد البويضات AFC، وعدد البويضات المخصبة)، مع قيمة p > 0.05. وجدت الدراسة أن مستويات cfDNA و17-استراديول كان لها علاقة سلبية غير هامة (قيمة p > 0.05). وجدت دراستنا أن مستوى cfDNA في السائل الجريبي يرتبط بقوة بجودة الأجنة في اليوم الثالث. |
| 530 ## - ADDITIONAL PHYSICAL FORM AVAILABLE NOTE | |
| Issues CD | Issues also as CD. |
| 546 ## - LANGUAGE NOTE | |
| Text Language | Text in English and abstract in Arabic & English. |
| 650 #0 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | Lasers |
| 650 #0 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | الليزر |
| 653 #1 - INDEX TERM--UNCONTROLLED | |
| Uncontrolled term | Cell-free DNA |
| -- | Oocyte’s |
| -- | Embryo |
| -- | Blastomeres |
| -- | الحمض النووي الحر خارج الخلايا (cfDNA) جنين |
| -- | البويضات |
| 700 0# - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Mohamed Amr Hussien El-Noury |
| Relator term | thesis advisor. |
| 700 0# - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Osama Fekry Ahmed Al-Balah |
| Relator term | thesis advisor. |
| 700 0# - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Tamer Fouad Taha |
| Relator term | thesis advisor. |
| 700 0# - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Sondos Mohamed Mohamed Salem |
| Relator term | thesis advisor. |
| 900 ## - Thesis Information | |
| Grant date | 01-01-2025 |
| Supervisory body | Mohamed Amr Hussien El-Noury |
| -- | Osama Fekry Ahmed Al-Balah |
| -- | Tamer Fouad Taha |
| -- | Sondos Mohamed Mohamed Salem |
| Universities | Cairo University |
| Faculties | National Institution of Laser Enhanced Sciences |
| Department | Department of Medical applications of laser Specialty ; laser applications in Gynecology |
| 905 ## - Cataloger and Reviser Names | |
| Cataloger Name | Shimaa |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) | |
| Source of classification or shelving scheme | Dewey Decimal Classification |
| Koha item type | Thesis |
| Edition | 21 |
| Suppress in OPAC | No |
| Source of classification or shelving scheme | Home library | Current library | Date acquired | Inventory number | Full call number | Barcode | Date last seen | Effective from | Koha item type |
|---|---|---|---|---|---|---|---|---|---|
| Dewey Decimal Classification | المكتبة المركزبة الجديدة - جامعة القاهرة | قاعة الرسائل الجامعية - الدور الاول | 19.04.2026 | 93816 | Cai01.24.03.Ph.D.2025.Ra.L | 01010110093816000 | 19.04.2026 | 19.04.2026 | Thesis |