Biochemical studies of caper bush capparis spinosa l. by in vitro culture / (رقم التسجيلة. 179683)
[ عرض عادي ]
| 000 -LEADER | |
|---|---|
| fixed length control field | 06268namaa22004451i 4500 |
| 003 - CONTROL NUMBER IDENTIFIER | |
| control field | EG-GICUC |
| 005 - أخر تعامل مع التسجيلة | |
| control field | 20260422182152.0 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION | |
| fixed length control field | 260422s2025 ua a|||frm||| 000 0 eng d |
| 040 ## - CATALOGING SOURCE | |
| Original cataloguing agency | EG-GICUC |
| Language of cataloging | eng |
| Transcribing agency | EG-GICUC |
| Modifying agency | EG-GICUC |
| Description conventions | rda |
| 041 0# - LANGUAGE CODE | |
| Language code of text/sound track or separate title | eng |
| Language code of summary or abstract | eng |
| -- | ara |
| 049 ## - Acquisition Source | |
| Acquisition Source | Deposit |
| 082 04 - DEWEY DECIMAL CLASSIFICATION NUMBER | |
| Classification number | 632.95 |
| 092 ## - LOCALLY ASSIGNED DEWEY CALL NUMBER (OCLC) | |
| Classification number | 632.95 |
| Edition number | 21 |
| 097 ## - Degree | |
| Degree | M.Sc |
| 099 ## - LOCAL FREE-TEXT CALL NUMBER (OCLC) | |
| Local Call Number | Cai01.07.04.M.Sc.2025.Fa.B |
| 100 0# - MAIN ENTRY--PERSONAL NAME | |
| Authority record control number or standard number | Fatma El-Zahraa Samir Sobhy, |
| Preparation | preparation. |
| 245 10 - TITLE STATEMENT | |
| Title | Biochemical studies of caper bush capparis spinosa l. by in vitro culture / |
| Statement of responsibility, etc. | by Fatma El-Zahraa Samir Sobhy ; Supervisors Dr. Sherif Helmy Ahmedm, Dr. Rania Saber Yousef, Dr.Shreif Said Saleh Ahmed. |
| 246 15 - VARYING FORM OF TITLE | |
| Title proper/short title | دراسات كيميائية حيوية علي نبات الكباريس باستخدام زراعة الانسجة |
| 264 #0 - PRODUCTION, PUBLICATION, DISTRIBUTION, MANUFACTURE, AND COPYRIGHT NOTICE | |
| Date of production, publication, distribution, manufacture, or copyright notice | 2025. |
| 300 ## - PHYSICAL DESCRIPTION | |
| Extent | 103 pages : |
| Other physical details | illustrations ; |
| Dimensions | 25 cm. + |
| Accompanying material | CD. |
| 336 ## - CONTENT TYPE | |
| Content type term | text |
| Source | rda content |
| 337 ## - MEDIA TYPE | |
| Media type term | Unmediated |
| Source | rdamedia |
| 338 ## - CARRIER TYPE | |
| Carrier type term | volume |
| Source | rdacarrier |
| 502 ## - DISSERTATION NOTE | |
| Dissertation note | Thesis (M.Sc)-Cairo University, 2025. |
| 504 ## - BIBLIOGRAPHY, ETC. NOTE | |
| Bibliography, etc. note | Bibliography: pages 87-103. |
| 520 #3 - SUMMARY, ETC. | |
| Summary, etc. | Capparis spinosa L., an edible medicinal plant, is rich in flavonoids. Production of <br/>secondary metabolites preferentially uses amino acids as precursors using in vitro culture <br/>techniques. This study aimed to evaluate the effect of amino acids as elicitors on <br/>morphological characterization,non-antioxidant compounds accumilation, antioxidant <br/>enzymes, and anticancer activity Capparis spinosa was in vitro cultured on MS media <br/>containing three amino acids included; tryptophan, glutamine or phenylalanine at 100, <br/>200, and 300 mg/L and compared with free amino acid culture (as control). After four <br/>weeks morphological characteristics were determined, some phytochemicals panel and <br/>rutin were evaluated by spectrophotometric and HPLC, respectively. The hepatocellular <br/>carcinoma cell line HepG2 was utilized to validate the anti cancer activity and in silico <br/>studies. The results showed that using colorex 15% resulted in 91.67% of <br/>disinfection.Applying 6-Benzylaminopurine(BAP) at 0.8 mg/L in MS media gave the <br/>highest shootlet length(4.03cm) ,but kinetin at 04 mg/L gave the highest shootlet number <br/>(6.11shoot/explant) . the most significant result (100%) to callus induction was achieved <br/>with MS medium supplemented with 0.5 mg/L 2,4-D for leaves.The MS media <br/>supplemented with 200 mg/L of tryptophan produced the most significant results, with an <br/>average shootlet number of 34.67. The explants grown on MS media supplemented with <br/>100 mg/L of phenylalanine showed the greatest shootlet length (6.83 cm). Moreover, the <br/>explant grown on MS media with 200 mg/L of tryptophan had the largest number of <br/>leaves (16.32).Regarding fresh weight, the control group recorded the highest fresh plant <br/>weight 3.50 g, while the highest fresh weight of callus was 4.7 g under the treatment with <br/>tryptophan at 100 mg/L or glutamine at 300 mg/L. .The HPLC analysis of rutin for the <br/>callus treated with glutamine at 100 mg/L or phenylalanine 200 mg/L (the highest <br/>treatments of flavonoids) scored 88.2 and 64.3 µg/g, respectively. The toxicity of callus <br/>extract treated with glutamine at 100 mg/L was more toxic for cancer cells (HepG2) <br/>followed by phenylalanine at 200 mg/L at IC50 356.65 and 371.05 µg/mL, respectively. <br/>Additionally, in molecular docking, rutin effectively reduced the activity in the HepG2 <br/>promoter. It downregulated the mRNA expression of HepG2-responsive genes of liver <br/>cancer cells, reducing its capacity to form spheroids and blocking the HepG2 signaling <br/>pathway. These findings suggest that amino acid elicitors can improve the metabolism of <br/>Capparis spinosa L. and could be used to produce high amounts of rutin, which has <br/>antitumor activity against HepG2. Preventing oxidative damage and inducing apoptosis <br/>may be the mechanisms responsible for the anti-tumor effect. Rutin from Capparis <br/>spinosa presents a promising candidate for further in silico and in vitro anticancer <br/>research, particularly through molecular docking studies to explore its interactions with <br/>key oncogenic targets. |
| 520 #3 - SUMMARY, ETC. | |
| Summary, etc. | هدفت الدراسة إلى اختبار تأثير ثلاثة أحماض أمينية (تربتوفان، جلوتامين، فينيل ألانين) على نمو نبات الكباريس وإنتاج مركباته الثانوية ونشاطه المضاد للسرطان. أثبت محلول الكلوروكس 15% أعلى كفاءة تعقيم، بينما حقق BAP أعلى طول للساق والكينيتين أعلى عدد للأفرع. وأظهر التربتوفان 200 ملجم/لتر أفضل نمو من حيث عدد الأفرع، في حين أعطى الفينيل ألانين 100 ملجم/لتر أطول الأفرع. سجّل الجلوتامين 100 ملجم/لتر والفينيل ألانين 200 ملجم/لتر أعلى محتوى من الروتين. وأظهر الكالس المعالج بالجلوتامين 100 ملجم/لتر أقوى تأثير مضاد لخلايا سرطان الكبد HepG2. كما بيّن الربط الجزيئي قدرة الروتين على خفض التعبير الجيني للخلايا السرطانية وإيقاف مسار إشاراتها، مما يجعله مرشحًا واعدًا لأبحاث علاج السرطان. |
| 530 ## - ADDITIONAL PHYSICAL FORM AVAILABLE NOTE | |
| Issues CD | Issues also as CD. |
| 546 ## - LANGUAGE NOTE | |
| Text Language | Text in English and abstract in Arabic & English. |
| 650 #0 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | Agricultural Biochemistry |
| 650 #0 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | الكيمياء الحيوية الزراعية |
| 653 #1 - INDEX TERM--UNCONTROLLED | |
| Uncontrolled term | Caper |
| -- | Anti cancer activity |
| -- | Tissue culture |
| -- | Flavonoids |
| -- | Secondary metabolites |
| -- | Molecular docking |
| -- | النشاط المضاد للسرطان |
| -- | الكباريس |
| 700 0# - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Sherif Helmy Ahmedm |
| Relator term | thesis advisor. |
| 700 0# - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Rania Saber Yousef |
| Relator term | thesis advisor. |
| 700 0# - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Shreif Said Saleh Ahmed |
| Relator term | thesis advisor. |
| 900 ## - Thesis Information | |
| Grant date | 01-01-2025 |
| Supervisory body | Sherif Helmy Ahmedm |
| -- | Rania Saber Yousef |
| -- | Shreif Said Saleh Ahmed |
| Discussion body | Amira Shawky Soliman |
| -- | Hanaa Fawzy Mohamed |
| Universities | Cairo University |
| Faculties | Faculty of Agriculture |
| Department | Department of Agricultural Biochemistry |
| 905 ## - Cataloger and Reviser Names | |
| Cataloger Name | Shimaa |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) | |
| Source of classification or shelving scheme | Dewey Decimal Classification |
| Koha item type | Thesis |
| Edition | 21 |
| Suppress in OPAC | No |
| Source of classification or shelving scheme | Home library | Current library | Date acquired | Inventory number | Full call number | Barcode | Date last seen | Effective from | Koha item type |
|---|---|---|---|---|---|---|---|---|---|
| Dewey Decimal Classification | المكتبة المركزبة الجديدة - جامعة القاهرة | قاعة الرسائل الجامعية - الدور الاول | 22.04.2026 | 93860 | Cai01.07.04.M.Sc.2025.Fa.B | 01010110093860000 | 22.04.2026 | 22.04.2026 | Thesis |