Isolation of the dehydrin 1 (Dh1) gene from mangrove (Avicennia marina L) and transformation in apricot / (رقم التسجيلة. 179684)
[ عرض عادي ]
| 000 -LEADER | |
|---|---|
| fixed length control field | 05961namaa22004451i 4500 |
| 003 - CONTROL NUMBER IDENTIFIER | |
| control field | EG-GICUC |
| 005 - أخر تعامل مع التسجيلة | |
| control field | 20260422183609.0 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION | |
| fixed length control field | 260422s2025 ua a|||frm||| 000 0 eng d |
| 040 ## - CATALOGING SOURCE | |
| Original cataloguing agency | EG-GICUC |
| Language of cataloging | eng |
| Transcribing agency | EG-GICUC |
| Modifying agency | EG-GICUC |
| Description conventions | rda |
| 041 0# - LANGUAGE CODE | |
| Language code of text/sound track or separate title | eng |
| Language code of summary or abstract | eng |
| -- | ara |
| 049 ## - Acquisition Source | |
| Acquisition Source | Deposit |
| 082 04 - DEWEY DECIMAL CLASSIFICATION NUMBER | |
| Classification number | 576.5 |
| 092 ## - LOCALLY ASSIGNED DEWEY CALL NUMBER (OCLC) | |
| Classification number | 576.5 |
| Edition number | 21 |
| 097 ## - Degree | |
| Degree | Ph.D |
| 099 ## - LOCAL FREE-TEXT CALL NUMBER (OCLC) | |
| Local Call Number | Cai01.07.10.Ph.D.2025.Ra.I |
| 100 0# - MAIN ENTRY--PERSONAL NAME | |
| Authority record control number or standard number | Rasha Ahmed Ahmed Ali Elmoreigi, |
| Preparation | preparation. |
| 245 10 - TITLE STATEMENT | |
| Title | Isolation of the dehydrin 1 (Dh1) gene from mangrove (Avicennia marina L) and transformation in apricot / |
| Statement of responsibility, etc. | by Rasha Ahmed Ahmed Ali Elmoreigi ; Supervisors Dr. Ebtissam Hussein Aly Hussein, Dr. Shereen Abu El-Maaty Mohamed, Dr. Sabah Anwar Hassanen. |
| 246 15 - VARYING FORM OF TITLE | |
| Title proper/short title | عزل جين الديهيدرين 1 (Dh1) من المانجروف (Avicennia marina L.) ونقله إلى أصل من المشمش (Prunus armeniaca L.) |
| 264 #0 - PRODUCTION, PUBLICATION, DISTRIBUTION, MANUFACTURE, AND COPYRIGHT NOTICE | |
| Date of production, publication, distribution, manufacture, or copyright notice | 2025. |
| 300 ## - PHYSICAL DESCRIPTION | |
| Extent | 152 pages : |
| Other physical details | illustrations ; |
| Dimensions | 25 cm. + |
| Accompanying material | CD. |
| 336 ## - CONTENT TYPE | |
| Content type term | text |
| Source | rda content |
| 337 ## - MEDIA TYPE | |
| Media type term | Unmediated |
| Source | rdamedia |
| 338 ## - CARRIER TYPE | |
| Carrier type term | volume |
| Source | rdacarrier |
| 502 ## - DISSERTATION NOTE | |
| Dissertation note | Thesis (Ph.D)-Cairo University, 2025. |
| 504 ## - BIBLIOGRAPHY, ETC. NOTE | |
| Bibliography, etc. note | Bibliography: pages 134-152. |
| 520 #3 - SUMMARY, ETC. | |
| Summary, etc. | Improvement of fruit trees to withstand abiotic stress is becoming a major <br/>goal to mitigate the threatening effects of the climatic changes on fruits <br/>productivity. Apricot (Prunus armeniaca L.) is one of the most economic <br/>deciduous stone fruit crops globally. This study aimed to develop an efficient in <br/>vitro regeneration protocol for the Egyptian Al-Amar apricot rootstock, and laying <br/>the foundation for its genetic enhancement through introducing a dehydrin gene <br/>isolated from mangrove (Avicennia marina L.) via the agrobacterium-mediated <br/>transformation system. Two types of explants, cotyledons and hypocotyls, were <br/>cultured on 48 woody plant medium (WPM) with various concentrations of TDZ, <br/>IBA or NAA to optimize shoot induction and regeneration. Cotyledons exhibited <br/>direct somatic organogenesis, with the highest bud induction (78%) achieved on <br/>WPM containing 13.62 µM TDZ + 2.46 µM IBA. While, hypocotyls underwent <br/>indirect regeneration through callus formation, with the callusing rate (96.87%) on <br/>WPM supplemented with 15.89 µM TDZ + 1.61 µM NAA. Shoot proliferation <br/>was most successful on media with 8.87 µM BAP and 0.54 µM NAA, with <br/>hypocotyls producing more shoots per explant than cotyledons. Optimal rooting <br/>occurred on WPM with 9.80 µM IBA. In parallel, the full-length of the dehydrin <br/>gene DHN was isolated from Avicennia marina via RT-PCR and cloned into <br/>pGEM-T Easy vector for sequencing and subsequently cloned into the pRI 201-AN <br/>plant expression vector. This construct was introduced into Agrobacterium <br/>tumefaciens LBA4404. Al-Amar cotyledon and hypocotyl explants were co-<br/>cultivated with Agrobacterium, followed by selection on media containing <br/>kanamycin and cefotaxime. For cotyledons, the highest survival rate (54.1%) was <br/>observed with 500 mg/l cefotaxime and 100 mg/l kanamycin and buds induction of <br/>20.9% was achieved on 400 mg/l cefotaxime and 50 mg/l kanamycin. In contrast, <br/>hypocotyls showed better survival on 500 mg/l cefotaxime and 50 mg/l kanamycin, <br/>but had limited callus formation. PCR screening confirmed the presence of the <br/>DHN gene in transformed tissues. The transformation efficiency of buds derived <br/>from cotyledons and calli induced from hypocotyls was 1.04% and 0.50%, <br/>respectively. This study represents the first attempt to develop an Egyptian <br/>transgenic apricot rootstock with improved salinity tolereance which could pave <br/>the way for producing non-transgenic apricot fruits under adverse conditions via <br/>transgrafting. |
| 520 #3 - SUMMARY, ETC. | |
| Summary, etc. | تم تأسيس برتوكول فعال لإعادة التكشف معملياً لأصل المشمش المصري (العمار) باستخدام نوعين من المنفصلات النباتية وهي: الفلقات والتي أظهرت تخليق الأعضاء الجسدية المباشر دون تكوين كالس وقد بلغت أعلى نسبة لتكوين البراعم (78%) و المنفصلات السويقة الجنينية والتي اتجهت للتخليق الغير مباشر للأعضاء النباتية من خلال تكوين الكالس ثم البراعم حيث بلغت أعلى نسبة لتحفيز انتاج الكالس (96.87%)، بالتوازي تم عزل جين الديهيدرين (DHN) الكامل من نبات المانجروف باستخدام تقنية النسخ العكسي (RT-PCR) ، وكلونته في ناقل التعبير الجيني في النبات pRI 201-AN ثم ادخاله إلى بكتريا الأجروباكتريم والتي استخدمت لاحقاً في التحول الوراثي لأصل المشمش "العمار" و بلغت كفاءة التحول الوراثي للبراعم الناتجة من الفلقات والكالس الناتج من السويقات الجنينية 1.04% و 0.50% على التوالي. |
| 530 ## - ADDITIONAL PHYSICAL FORM AVAILABLE NOTE | |
| Issues CD | Issues also as CD. |
| 546 ## - LANGUAGE NOTE | |
| Text Language | Text in English and abstract in Arabic & English. |
| 650 #0 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | Genetics |
| 650 #0 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | الوراثة |
| 653 #1 - INDEX TERM--UNCONTROLLED | |
| Uncontrolled term | Apricot rootstock |
| -- | Cotyledon |
| -- | Hypocotyl |
| -- | Explant type, |
| -- | Prunus armeniaca |
| -- | in vitro regeneration |
| -- | DHN gene |
| -- | Avicennia marina |
| -- | إعادة تكشف النباتات معملياً |
| -- | أصل المشمش |
| 700 0# - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Ebtissam Hussein Aly Hussein |
| Relator term | thesis advisor. |
| 700 0# - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Shereen Abu El-Maaty Mohamed |
| Relator term | thesis advisor. |
| 700 0# - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Sabah Anwar Hassanen |
| Relator term | thesis advisor. |
| 900 ## - Thesis Information | |
| Grant date | 01-01-2025 |
| Supervisory body | Ebtissam Hussein Aly Hussein |
| -- | Shereen Abu El-Maaty Mohamed |
| -- | Sabah Anwar Hassanen |
| Discussion body | Mohamed Abd Elsalam Rashed |
| -- | Salah Eldin Sayed Mohamed Elassal |
| Universities | Cairo University |
| Faculties | Faculty of Agriculture |
| Department | Department of Genetics |
| 905 ## - Cataloger and Reviser Names | |
| Cataloger Name | Shimaa |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) | |
| Source of classification or shelving scheme | Dewey Decimal Classification |
| Koha item type | Thesis |
| Edition | 21 |
| Suppress in OPAC | No |
| Source of classification or shelving scheme | Home library | Current library | Date acquired | Inventory number | Full call number | Barcode | Date last seen | Effective from | Koha item type |
|---|---|---|---|---|---|---|---|---|---|
| Dewey Decimal Classification | المكتبة المركزبة الجديدة - جامعة القاهرة | قاعة الرسائل الجامعية - الدور الاول | 22.04.2026 | 93861 | Cai01.07.10.Ph.D.2025.Ra.I | 01010110093861000 | 22.04.2026 | 22.04.2026 | Thesis |