Production of monoclonal antibodies-based ELISA kits for pathogenic Leptospira spp. / (Record no. 47387)

MARC details
000 -LEADER
fixed length control field 02231cam a2200349 a 4500
003 - CONTROL NUMBER IDENTIFIER
control field EG-GiCUC
005 - DATE AND TIME OF LATEST TRANSACTION
control field 20250223031041.0
008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION
fixed length control field 140916s2014 ua h f m 000 0 eng d
040 ## - CATALOGING SOURCE
Original cataloging agency EG-GiCUC
Language of cataloging eng
Transcribing agency EG-GiCUC
041 0# - LANGUAGE CODE
Language code of text/sound track or separate title eng
049 ## - LOCAL HOLDINGS (OCLC)
Holding library Deposite
097 ## - Thesis Degree
Thesis Level Ph.D
099 ## - LOCAL FREE-TEXT CALL NUMBER (OCLC)
Classification number Cai01.10.10.Ph.D.2014.Re.P
100 0# - MAIN ENTRY--PERSONAL NAME
Personal name Rehab Ali Mohammed Mohammed Elhelw
245 10 - TITLE STATEMENT
Title Production of monoclonal antibodies-based ELISA kits for pathogenic Leptospira spp. /
Statement of responsibility, etc. Rehab Ali Mohammed Mohammed Elhelw ; Supervised Mahmoud Essam Hatem Ahmed , Mona Ibrahim Elnbaawy , Ahmed Abdelghani Elsanousi
246 15 - VARYING FORM OF TITLE
Title proper/short title إنتاج مجموعات إليزا علي أساس الأجسام المضادة أحادية الفسيلة لليبتوسبيرا الممرضة
260 ## - PUBLICATION, DISTRIBUTION, ETC.
Place of publication, distribution, etc. Cairo :
Name of publisher, distributor, etc. Rehab Ali Mohammed Mohammed Elhelw ,
Date of publication, distribution, etc. 2014
300 ## - PHYSICAL DESCRIPTION
Extent 184 P. :
Other physical details facsimiles ;
Dimensions 25cm
502 ## - DISSERTATION NOTE
Dissertation note Thesis (Ph.D.) - Cairo University - Faculty of Veterinary Medicine - Department of Microbiology
520 ## - SUMMARY, ETC.
Summary, etc. A panel of monoclonal antibodies (MAbs) against each of Leptospira interrogans serovar Canicola and pathogenic Leptospira spp. were produced. L. interrogans serovar Canicola was cultivated, concentrated and prepared for fusion. BALB/c mice of 6 weeks old were inoculated with different antigenic preparations of L. interrogans serovar Canicola with complete freund{u2019}s adjuvant. Booster doses with and without incomplete freund{u2019}s adjuvant were applied. To obtain hybridomas, mice spleen cells were fused with myeloma cells in the presence of polyethylene glycol. Hybridoma cells that secret specific antibodies were selected on the basis of ELISA. These positive hybridomas were cloned by limiting dilution method to reveal subclones of one cell origin. 5 specific clones were obtained
530 ## - ADDITIONAL PHYSICAL FORM AVAILABLE NOTE
Additional physical form available note Issued also as CD
653 #4 - INDEX TERM--UNCONTROLLED
Uncontrolled term MAbs
653 #4 - INDEX TERM--UNCONTROLLED
Uncontrolled term Pathogenic Leptospira spp.
653 #4 - INDEX TERM--UNCONTROLLED
Uncontrolled term Serovar Canicola
700 0# - ADDED ENTRY--PERSONAL NAME
Personal name Ahmed Abdelghani Elsanousi ,
Relator term
700 0# - ADDED ENTRY--PERSONAL NAME
Personal name Mahmoud Essam Hatem Ahmed ,
Relator term
700 0# - ADDED ENTRY--PERSONAL NAME
Personal name Mona Ibrahim Elnbaawy ,
Relator term
856 ## - ELECTRONIC LOCATION AND ACCESS
Uniform Resource Identifier <a href="http://172.23.153.220/th.pdf">http://172.23.153.220/th.pdf</a>
905 ## - LOCAL DATA ELEMENT E, LDE (RLIN)
Cataloger Nazla
Reviser Revisor
905 ## - LOCAL DATA ELEMENT E, LDE (RLIN)
Cataloger Soheir
Reviser Cataloger
942 ## - ADDED ENTRY ELEMENTS (KOHA)
Source of classification or shelving scheme Dewey Decimal Classification
Koha item type Thesis
Holdings
Source of classification or shelving scheme Not for loan Home library Current library Date acquired Full call number Barcode Date last seen Koha item type Copy number
Dewey Decimal Classification   المكتبة المركزبة الجديدة - جامعة القاهرة قاعة الرسائل الجامعية - الدور الاول 11.02.2024 Cai01.10.10.Ph.D.2014.Re.P 01010110063359000 22.09.2023 Thesis  
Dewey Decimal Classification   المكتبة المركزبة الجديدة - جامعة القاهرة مخـــزن الرســائل الجـــامعية - البدروم 11.02.2024 Cai01.10.10.Ph.D.2014.Re.P 01020110063359000 22.09.2023 CD - Rom 63359.CD
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