Transient expression of ELN gene in tobacco plant / (Record no. 54187)
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| 000 -LEADER | |
|---|---|
| fixed length control field | 02994cam a2200349 a 4500 |
| 003 - CONTROL NUMBER IDENTIFIER | |
| control field | EG-GiCUC |
| 005 - DATE AND TIME OF LATEST TRANSACTION | |
| control field | 20250223031400.0 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION | |
| fixed length control field | 151229s2015 ua do f m 000 0 eng d |
| 040 ## - CATALOGING SOURCE | |
| Original cataloging agency | EG-GiCUC |
| Language of cataloging | eng |
| Transcribing agency | EG-GiCUC |
| 041 0# - LANGUAGE CODE | |
| Language code of text/sound track or separate title | eng |
| 049 ## - LOCAL HOLDINGS (OCLC) | |
| Holding library | Deposite |
| 097 ## - Thesis Degree | |
| Thesis Level | M.Sc |
| 099 ## - LOCAL FREE-TEXT CALL NUMBER (OCLC) | |
| Classification number | Cai01.07.10.M.Sc.2015.Mo.T |
| 100 0# - MAIN ENTRY--PERSONAL NAME | |
| Personal name | Mona Ahmed Mohamed Abdelghani |
| 245 10 - TITLE STATEMENT | |
| Title | Transient expression of ELN gene in tobacco plant / |
| Statement of responsibility, etc. | Mona Ahmed Mohamed Abdelghani ; Supervised Naglaa Abdelmoneam Abdallah , Abdel Hadi Abdallah , Ghada Aboelheba |
| 246 15 - VARYING FORM OF TITLE | |
| Title proper/short title | فى نبات الدخان ELNالتعبير المؤقت لجين ال |
| 260 ## - PUBLICATION, DISTRIBUTION, ETC. | |
| Place of publication, distribution, etc. | Cairo : |
| Name of publisher, distributor, etc. | Mona Ahmed Mohamed Abdelghani , |
| Date of publication, distribution, etc. | 2015 |
| 300 ## - PHYSICAL DESCRIPTION | |
| Extent | 67 P. : |
| Other physical details | charts , photographs ; |
| Dimensions | 25cm |
| 502 ## - DISSERTATION NOTE | |
| Dissertation note | Thesis (M.Sc.) - Cairo University - Faculty of Agriculture - Department of Genetics |
| 520 ## - SUMMARY, ETC. | |
| Summary, etc. | Plant molecular farming (PMF) is an important growing prospective approach in plant biotechnology. It includes production of recombinant pharmaceutical and industrial proteins in large quantities from engineered plants. Elastin is a major protein component of elastic fibers. They provide tissues with the required resilience and elasticity. In this work, Nicotiana tabacum leaves were used as a bioreactor for the expression and production of the full human tropoelastin protein; the precursor protein of elastin. The agrobacterium- mediated transient expression system into Nicotiana tabacum was used via syringe agroinfiltration to provide a fast and convenient way to produce recombinant proteins with a greater expression overall the plant leaf. This study aimed to establish an efficient and rapid system for transiently expression and production of human recombinant tropoelastin protein in transgenic N. tabacum plants. Modified sequence of the elastin (ELN) gene was biosynthesized and cloned into pCambia1390 vector to be transferred into N. tabacum. Optimization of codon usage for the human tropoelastin gene, without changing the primary structure of the protein was carried out to ensure high expression in tobacco plants. The obtained data proved that the 5th day post-infiltration is the optimum interval to obtain the maximum production of the recombinant protein. Southern blot analysis was able to detect the 2175 bps fragment representing the ELN orf. On the other hand, ELN -expression within plant{u2019}s tissue was visualized by RT-PCR within 3 to 10 days post agroinfiltration. At the protein level, western and ELISA confirmed the expression of recombinant tropoelastin protein |
| 530 ## - ADDITIONAL PHYSICAL FORM AVAILABLE NOTE | |
| Additional physical form available note | Issued also as CD |
| 653 #4 - INDEX TERM--UNCONTROLLED | |
| Uncontrolled term | Agroinfiltration |
| 653 #4 - INDEX TERM--UNCONTROLLED | |
| Uncontrolled term | Molecular farming |
| 653 #4 - INDEX TERM--UNCONTROLLED | |
| Uncontrolled term | Transient expression |
| 700 0# - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Abdelhadi Abdallah , |
| Relator term | |
| 700 0# - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Ghada Aboelheba , |
| Relator term | |
| 700 0# - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Naglaa Abdelmonem Abdullah , |
| Relator term | |
| 856 ## - ELECTRONIC LOCATION AND ACCESS | |
| Uniform Resource Identifier | <a href="http://172.23.153.220/th.pdf">http://172.23.153.220/th.pdf</a> |
| 905 ## - LOCAL DATA ELEMENT E, LDE (RLIN) | |
| Cataloger | Enas |
| Reviser | Cataloger |
| 905 ## - LOCAL DATA ELEMENT E, LDE (RLIN) | |
| Cataloger | Nazla |
| Reviser | Revisor |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) | |
| Source of classification or shelving scheme | Dewey Decimal Classification |
| Koha item type | Thesis |
| Source of classification or shelving scheme | Not for loan | Home library | Current library | Date acquired | Full call number | Barcode | Date last seen | Koha item type | Copy number |
|---|---|---|---|---|---|---|---|---|---|
| Dewey Decimal Classification | المكتبة المركزبة الجديدة - جامعة القاهرة | قاعة الرسائل الجامعية - الدور الاول | 11.02.2024 | Cai01.07.10.M.Sc.2015.Mo.T | 01010110067366000 | 22.09.2023 | Thesis | ||
| Dewey Decimal Classification | المكتبة المركزبة الجديدة - جامعة القاهرة | مخـــزن الرســائل الجـــامعية - البدروم | 11.02.2024 | Cai01.07.10.M.Sc.2015.Mo.T | 01020110067366000 | 22.09.2023 | CD - Rom | 67366.CD |