Molecular genetic studies on a midgut related gene in pink bollworm - pectinophora gossypiella / (Record no. 54211)
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| 000 -LEADER | |
|---|---|
| fixed length control field | 02841cam a2200349 a 4500 |
| 003 - CONTROL NUMBER IDENTIFIER | |
| control field | EG-GiCUC |
| 005 - DATE AND TIME OF LATEST TRANSACTION | |
| control field | 20250223031401.0 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION | |
| fixed length control field | 160102s2015 ua ho f m 000 0 eng d |
| 040 ## - CATALOGING SOURCE | |
| Original cataloging agency | EG-GiCUC |
| Language of cataloging | eng |
| Transcribing agency | EG-GiCUC |
| 041 0# - LANGUAGE CODE | |
| Language code of text/sound track or separate title | eng |
| 049 ## - LOCAL HOLDINGS (OCLC) | |
| Holding library | Deposite |
| 097 ## - Thesis Degree | |
| Thesis Level | M.Sc |
| 099 ## - LOCAL FREE-TEXT CALL NUMBER (OCLC) | |
| Classification number | Cai01.07.10.M.Sc.2015.Me.M |
| 100 0# - MAIN ENTRY--PERSONAL NAME | |
| Personal name | Mervat Ragab Mansour Ali Diab |
| 245 10 - TITLE STATEMENT | |
| Title | Molecular genetic studies on a midgut related gene in pink bollworm - pectinophora gossypiella / |
| Statement of responsibility, etc. | Mervat Ragab Mansour Ali Diab ; Supervised Ebtissam Hussein Aly Hussein , Samir Mohamed Mustafa Abdalla , Ahmed Mohammed Ahmed Mohammed |
| 246 15 - VARYING FORM OF TITLE | |
| Title proper/short title | دراسات وراثية على جين بالمعدة الوسطى لدودة اللوز القرنفلية |
| 260 ## - PUBLICATION, DISTRIBUTION, ETC. | |
| Place of publication, distribution, etc. | Cairo : |
| Name of publisher, distributor, etc. | Mervat Ragab Mansour Ali Diab , |
| Date of publication, distribution, etc. | 2015 |
| 300 ## - PHYSICAL DESCRIPTION | |
| Extent | 115 P. : |
| Other physical details | facsimiles , photographs ; |
| Dimensions | 25cm |
| 502 ## - DISSERTATION NOTE | |
| Dissertation note | Thesis (M.Sc.) - Cairo University - Faculty of Agriculture - Department of Genetics |
| 520 ## - SUMMARY, ETC. | |
| Summary, etc. | Vacuolar - H⁺ ATPase (V - ATPase) is a multi subunit enzyme. It consists of two domains (V₁ and V₀). V₁ domain has eight subunits (A - H) while VO domain has six subunits (a, c, c', c'', d and e). The V - ATPase is an ATP - driven proton pump and is essential to the function of eukaryotic cells. In insects, V - ATPase is located in apical membrane of goblet cells in the midgut. It acts as an energizer of the plasma membrane, driving nutrient uptake, fluid secretion and in some cases alkalizing the gut lumen. The aim of the present study was knocking down the V - ATPase gene(s) using RNAi in an attempt to disturb the food process within the pink bollworm (PBW) midgut and eventually causing the insect death. Based on the conserved regions of the V - ATPase subunits A and D genes in different insects available in the Gen Bank, degenerate primers were synthesized and used to amplify the two genes. The amplified genes encoding the V - ATPase subunits A and D transcripts were sequenced. The sequencing results confirmed the isolation of the full length genes encoding the V - ATPase subunit A was 2602 bp in length, encoding 618 amino acids. While, the gene encoding V - ATPase subunit D was 1467 bp, encoding 249 amino acids. Three dsRNA fragments were designed, two of them (VATPA756 - 1155 bp and VATPA347-753 bp) were targeting subunit A and the third - VATPD221 - 703 bp to knockdown subunit D |
| 530 ## - ADDITIONAL PHYSICAL FORM AVAILABLE NOTE | |
| Additional physical form available note | Issued also as CD |
| 653 #4 - INDEX TERM--UNCONTROLLED | |
| Uncontrolled term | Pectinophora gossypiella |
| 653 #4 - INDEX TERM--UNCONTROLLED | |
| Uncontrolled term | Pink bollworm |
| 653 #4 - INDEX TERM--UNCONTROLLED | |
| Uncontrolled term | RNA interference |
| 700 0# - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Ahmed Mohammed Ahmed Mohammed , |
| Relator term | |
| 700 0# - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Ebtissam Hussein Aly Hussein , |
| Relator term | |
| 700 0# - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Samir Mohamed Mustafa Abdalla , |
| Relator term | |
| 856 ## - ELECTRONIC LOCATION AND ACCESS | |
| Uniform Resource Identifier | <a href="http://172.23.153.220/th.pdf">http://172.23.153.220/th.pdf</a> |
| 905 ## - LOCAL DATA ELEMENT E, LDE (RLIN) | |
| Cataloger | Nazla |
| Reviser | Revisor |
| 905 ## - LOCAL DATA ELEMENT E, LDE (RLIN) | |
| Cataloger | Samia |
| Reviser | Cataloger |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) | |
| Source of classification or shelving scheme | Dewey Decimal Classification |
| Koha item type | Thesis |
| Source of classification or shelving scheme | Not for loan | Home library | Current library | Date acquired | Full call number | Barcode | Date last seen | Koha item type | Copy number |
|---|---|---|---|---|---|---|---|---|---|
| Dewey Decimal Classification | المكتبة المركزبة الجديدة - جامعة القاهرة | قاعة الرسائل الجامعية - الدور الاول | 11.02.2024 | Cai01.07.10.M.Sc.2015.Me.M | 01010110067445000 | 22.09.2023 | Thesis | ||
| Dewey Decimal Classification | المكتبة المركزبة الجديدة - جامعة القاهرة | مخـــزن الرســائل الجـــامعية - البدروم | 11.02.2024 | Cai01.07.10.M.Sc.2015.Me.M | 01020110067445000 | 22.09.2023 | CD - Rom | 67445.CD |