Studies on phage-encoded streptodornases in streptococcus pyogenes clinical samples Isolated in Egypt / (Record no. 54838)
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| 000 -LEADER | |
|---|---|
| fixed length control field | 02540cam a2200337 a 4500 |
| 003 - CONTROL NUMBER IDENTIFIER | |
| control field | EG-GiCUC |
| 005 - DATE AND TIME OF LATEST TRANSACTION | |
| control field | 20250223031420.0 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION | |
| fixed length control field | 160201s2015 ua h f m 000 0 eng d |
| 040 ## - CATALOGING SOURCE | |
| Original cataloging agency | EG-GiCUC |
| Language of cataloging | eng |
| Transcribing agency | EG-GiCUC |
| 041 0# - LANGUAGE CODE | |
| Language code of text/sound track or separate title | eng |
| 049 ## - LOCAL HOLDINGS (OCLC) | |
| Holding library | Deposite |
| 097 ## - Thesis Degree | |
| Thesis Level | M.Sc |
| 099 ## - LOCAL FREE-TEXT CALL NUMBER (OCLC) | |
| Classification number | Cai01.08.06.M.Sc.2015.Im.S |
| 100 0# - MAIN ENTRY--PERSONAL NAME | |
| Personal name | Iman Kamal Eldin Mohamed Fouad |
| 245 10 - TITLE STATEMENT | |
| Title | Studies on phage-encoded streptodornases in streptococcus pyogenes clinical samples Isolated in Egypt / |
| Statement of responsibility, etc. | Iman Kamal Eldin Mohamed Fouad ; Supervised Magdy Ali Amin , Ramy Karam Aziz , |
| 246 15 - VARYING FORM OF TITLE | |
| Title proper/short title | دراسات على إنزيمات الاسترپتودورنيز المحمولة على بكتيريوفاچ في عزلات المكورات السبحية من مصر |
| 260 ## - PUBLICATION, DISTRIBUTION, ETC. | |
| Place of publication, distribution, etc. | Cairo : |
| Name of publisher, distributor, etc. | Iman Kamal Eldin Mohamed Fouad , |
| Date of publication, distribution, etc. | 2015 |
| 300 ## - PHYSICAL DESCRIPTION | |
| Extent | 134 P. : |
| Other physical details | facsimiles ; |
| Dimensions | 25cm |
| 502 ## - DISSERTATION NOTE | |
| Dissertation note | Thesis (M.Sc.) - Cairo University - Faculty of Pharmacy - Department of Microbiology and Immunology |
| 520 ## - SUMMARY, ETC. | |
| Summary, etc. | Group A streptococci (GAS) are human pathogens causing 650,000 annual deaths among humans. Recently, hypervirulent, invasive GAS strains have emerged and their emergence was correlated with the carriage streptodornase gene variants, which encode secreted nucleases that degrade neutrophil extracellular traps, protecting the bacteria from phagocytosis. So far, five major streptodornase classes have been discovered, four of which are phage-encoded: Spd2, Spd3, Spd4, and Sda; the fifth is the chromosomally encoded Spd. To date, no method is available for rapid and accurate genotyping of all five classes in clinical GAS isolates. To rapidly investigate the distribution of phage-encoded streptodornases among clinical isolates, we initiated this study to develop a polymerase chain reaction (PCR)-based genotyping approach. First, a bioinformatics analysis was conducted to screen GAS genomes for known streptodornase patterns. Next, clinical isolates from Egypt were cultured and tested for nuclease activity by subculture on DNA-agar plates containing methylene blue, then by an agarose gel electrophoresis-based method to test the extent of DNA degradation |
| 530 ## - ADDITIONAL PHYSICAL FORM AVAILABLE NOTE | |
| Additional physical form available note | Issued also as CD |
| 653 #4 - INDEX TERM--UNCONTROLLED | |
| Uncontrolled term | Clinical samples Isolated |
| 653 #4 - INDEX TERM--UNCONTROLLED | |
| Uncontrolled term | Phage-encoded streptodornases |
| 653 #4 - INDEX TERM--UNCONTROLLED | |
| Uncontrolled term | Streptococcus pyogenes |
| 700 0# - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Magdy Ali Amin , |
| Relator term | |
| 700 0# - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Ramy Karam Aziz , |
| Relator term | |
| 856 ## - ELECTRONIC LOCATION AND ACCESS | |
| Uniform Resource Identifier | <a href="http://172.23.153.220/th.pdf">http://172.23.153.220/th.pdf</a> |
| 905 ## - LOCAL DATA ELEMENT E, LDE (RLIN) | |
| Cataloger | Aml |
| Reviser | Cataloger |
| 905 ## - LOCAL DATA ELEMENT E, LDE (RLIN) | |
| Cataloger | Nazla |
| Reviser | Revisor |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) | |
| Source of classification or shelving scheme | Dewey Decimal Classification |
| Koha item type | Thesis |
| Source of classification or shelving scheme | Not for loan | Home library | Current library | Date acquired | Full call number | Barcode | Date last seen | Koha item type | Copy number |
|---|---|---|---|---|---|---|---|---|---|
| Dewey Decimal Classification | المكتبة المركزبة الجديدة - جامعة القاهرة | قاعة الرسائل الجامعية - الدور الاول | 11.02.2024 | Cai01.08.06.M.Sc.2015.Im.S | 01010110067775000 | 22.09.2023 | Thesis | ||
| Dewey Decimal Classification | المكتبة المركزبة الجديدة - جامعة القاهرة | مخـــزن الرســائل الجـــامعية - البدروم | 11.02.2024 | Cai01.08.06.M.Sc.2015.Im.S | 01020110067775000 | 22.09.2023 | CD - Rom | 67775.CD |