Cloning and characterization of insecticidal protein encoding genes from the symbiotic bacteria with entomopathogenic nematode / (Record no. 55993)
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| 000 -LEADER | |
|---|---|
| fixed length control field | 02913cam a2200349 a 4500 |
| 003 - CONTROL NUMBER IDENTIFIER | |
| control field | EG-GiCUC |
| 005 - DATE AND TIME OF LATEST TRANSACTION | |
| control field | 20250223031454.0 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION | |
| fixed length control field | 160413s2015 ua h f m 000 0 eng d |
| 040 ## - CATALOGING SOURCE | |
| Original cataloging agency | EG-GiCUC |
| Language of cataloging | eng |
| Transcribing agency | EG-GiCUC |
| 041 0# - LANGUAGE CODE | |
| Language code of text/sound track or separate title | eng |
| 049 ## - LOCAL HOLDINGS (OCLC) | |
| Holding library | Deposite |
| 097 ## - Thesis Degree | |
| Thesis Level | M.Sc |
| 099 ## - LOCAL FREE-TEXT CALL NUMBER (OCLC) | |
| Classification number | Cai01.07.10.M.Sc.2015.Ba.C |
| 100 0# - MAIN ENTRY--PERSONAL NAME | |
| Personal name | Baiome Abdelmaguid Ali |
| 245 10 - TITLE STATEMENT | |
| Title | Cloning and characterization of insecticidal protein encoding genes from the symbiotic bacteria with entomopathogenic nematode / |
| Statement of responsibility, etc. | Baiome Abdelmaguid Ali ; Supervised Mohamed Hassanein Soliman , Reda Elwany Abedelhaleem Moghaieb , Hanan Abdelfattah Elsadawy |
| 246 15 - VARYING FORM OF TITLE | |
| Title proper/short title | عزل و توصيف احدى الجينات المسئولة عن انتاج السموم البكتيرية من البكتيريا المصاحبة للنيماتودا الممرضة للحشرات |
| 260 ## - PUBLICATION, DISTRIBUTION, ETC. | |
| Place of publication, distribution, etc. | Cairo : |
| Name of publisher, distributor, etc. | Baiome Abdelmaguid Ali , |
| Date of publication, distribution, etc. | 2015 |
| 300 ## - PHYSICAL DESCRIPTION | |
| Extent | 130 P. : |
| Other physical details | facsimiles ; |
| Dimensions | 25cm |
| 502 ## - DISSERTATION NOTE | |
| Dissertation note | Thesis (M.Sc.) - Cairo University - Faculty of Agriculture - Department of Genetics |
| 520 ## - SUMMARY, ETC. | |
| Summary, etc. | Three symbiotic bacterial strains isolated, from Heterorhabditis and two from Steinernema were identified as photorhabdusluminescens HRM1, P. luminescens HS1, P. luminescens HP88, Xenorhabdusindica and X. nematophila based on 16S rDNA sequence analysis. The genetic diversity among these bacterial strains was resolved using three molecular markers (RAPD, ISSR and SRAP). RAPD analysis showed 73.8 and 54.5 polymorphism percentages for the PhotorhabdusandXenorhabdusstrains respectively. ISSR analysis resulted in 70.1 and 75.2 polymorphism percentages among the Photorhabdus and Xenorhabdus strains, respectively. Twenty five SRAP combinations of five forward and five reverse primers were used to characterize these bacterial strain. The Photorhabdusstrains showed 75.6 polymorphism percentages among them while Xenorhabdus strains showed 61.2% polymorphism. Txp40 toxin gene from P. luminescens HP88 strain was identified. The data indicated that, the the sequence obtained was highly similar to the complete sequence of the txp40 genes of P. luminescens strains with the score of (99%) with P. luminescens TTO1 strain, (98%) with P. luminescens V16 strain and (98%) with P. luminescens HI strain. Total recombinant toxin protein was shown to be active against G. mellonella by direct injection into the larvae. Syringed proteins extracted from transformed E.coli DH5Ü strain killed a high percentage of G. mellonella larvae |
| 530 ## - ADDITIONAL PHYSICAL FORM AVAILABLE NOTE | |
| Additional physical form available note | Issued also as CD |
| 653 #4 - INDEX TERM--UNCONTROLLED | |
| Uncontrolled term | Photorhabdus |
| 653 #4 - INDEX TERM--UNCONTROLLED | |
| Uncontrolled term | RAPD |
| 653 #4 - INDEX TERM--UNCONTROLLED | |
| Uncontrolled term | Xenorhabdus |
| 700 0# - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Hanan Abdelfattah Elsadawy , |
| Relator term | |
| 700 0# - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Mohamed Hassanein Soliman , |
| Relator term | |
| 700 0# - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Reda Elwany Abedelhaleem Moghaieb , |
| Relator term | |
| 856 ## - ELECTRONIC LOCATION AND ACCESS | |
| Uniform Resource Identifier | <a href="http://172.23.153.220/th.pdf">http://172.23.153.220/th.pdf</a> |
| 905 ## - LOCAL DATA ELEMENT E, LDE (RLIN) | |
| Cataloger | Nazla |
| Reviser | Revisor |
| 905 ## - LOCAL DATA ELEMENT E, LDE (RLIN) | |
| Cataloger | Samia |
| Reviser | Cataloger |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) | |
| Source of classification or shelving scheme | Dewey Decimal Classification |
| Koha item type | Thesis |
| Source of classification or shelving scheme | Not for loan | Home library | Current library | Date acquired | Full call number | Barcode | Date last seen | Koha item type | Copy number |
|---|---|---|---|---|---|---|---|---|---|
| Dewey Decimal Classification | المكتبة المركزبة الجديدة - جامعة القاهرة | قاعة الرسائل الجامعية - الدور الاول | 11.02.2024 | Cai01.07.10.M.Sc.2015.Ba.C | 01010110068569000 | 22.09.2023 | Thesis | ||
| Dewey Decimal Classification | المكتبة المركزبة الجديدة - جامعة القاهرة | مخـــزن الرســائل الجـــامعية - البدروم | 11.02.2024 | Cai01.07.10.M.Sc.2015.Ba.C | 01020110068569000 | 22.09.2023 | CD - Rom | 68569.CD |