Effect of some anticancer agents on some metabolic pathways in cancer cell lines / (Record no. 56033)
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| 000 -LEADER | |
|---|---|
| fixed length control field | 03388cam a2200349 a 4500 |
| 003 - CONTROL NUMBER IDENTIFIER | |
| control field | EG-GiCUC |
| 005 - DATE AND TIME OF LATEST TRANSACTION | |
| control field | 20250223031455.0 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION | |
| fixed length control field | 160416s2015 ua dh f m 000 0 eng d |
| 040 ## - CATALOGING SOURCE | |
| Original cataloging agency | EG-GiCUC |
| Language of cataloging | eng |
| Transcribing agency | EG-GiCUC |
| 041 0# - LANGUAGE CODE | |
| Language code of text/sound track or separate title | eng |
| 049 ## - LOCAL HOLDINGS (OCLC) | |
| Holding library | Deposite |
| 097 ## - Thesis Degree | |
| Thesis Level | M.Sc |
| 099 ## - LOCAL FREE-TEXT CALL NUMBER (OCLC) | |
| Classification number | Cai01.08.01.M.Sc.2015.Sa.E |
| 100 0# - MAIN ENTRY--PERSONAL NAME | |
| Personal name | Sally Atef Tadros Fahim |
| 245 10 - TITLE STATEMENT | |
| Title | Effect of some anticancer agents on some metabolic pathways in cancer cell lines / |
| Statement of responsibility, etc. | Sally Atef Tadros Fahim ; Supervised Tarek Mohamed Kamal Motawi , Nermin Abdelhamid Sadik , Samia Abdelsamiaa Shouman |
| 246 15 - VARYING FORM OF TITLE | |
| Title proper/short title | دراسة كيموحيوية عن تأثير بعض المواد المضادة للسرطان على بعض المسارات ا{uئإئ٧}يضية فى الخلايا السرطانية |
| 260 ## - PUBLICATION, DISTRIBUTION, ETC. | |
| Place of publication, distribution, etc. | Cairo : |
| Name of publisher, distributor, etc. | Sally Atef Tadros Fahim , |
| Date of publication, distribution, etc. | 2015 |
| 300 ## - PHYSICAL DESCRIPTION | |
| Extent | 137 P. : |
| Other physical details | charts , facsimiles ; |
| Dimensions | 25cm |
| 502 ## - DISSERTATION NOTE | |
| Dissertation note | Thesis (M.Sc.) - Cairo University - Faculty of Pharmacy - Department of Biochemistry |
| 520 ## - SUMMARY, ETC. | |
| Summary, etc. | Imatinib mesylate (IM), a tyrosine kinase inhibitor, is used as targeted cancer therapy. However, mono-targeting by IM does not always achieve full tumor eradication and thus, it is recommended to combine IM with other anticancer agents. Clotrimazole (CLT) is an antifungal azole derivative with promising anticancer effects due to inhibiting the activity of glycolytic enzymes. The present study aimed to evaluate the effect of combining CLT with IM on breast cancer cell line in an attempt to establish effective new combination. T47D human breast cancer cell line was treated with different concentrations of IM and/or CLT for 48 hours. IM-CLT interaction was determined by isobologram equation and combination index. Cell viability was confirmed by measuring lactate dehydrogenase (LDH) activity. As indicators of glycolysis inhibition, the expression of hexokinase-2 (HK-2) and 6- phosphofructo-1-kinase (PFK-1) plus the activity of intracellular LDH and pyruvate kinase (PK) were determined. In addition, glucose consumption and ATP production were measured. Moreover, nitric oxide (NO), vascular endothelial growth factor (VEGF) and hypoxia inducible factor-Ü (HIF- Ü) were also determined as they are modulators for glycolysis. This study demonstrated that IM or CLT synergistically inhibited cell growth in T47D as shown by combination and dose reduction indices. The combination of 15æM IM and 20æM CLT significantly decreased glucose consumption, activity of both PK and intracellular LDH, while increased leaked LDH, VEGF and NO in the medium compared to each drug alone. Furthermore the combination decreased gene expression of HK-2, PFK-1 and ATP content compared to the control. In conclusion, the synergistic effect of CLT on IM cytotoxicity in T47D cell line maybe mediated through inhibition of glycolysis and increasing both NO and VEGF. Further studies are required to confirm the efficiency and safety of this combination |
| 530 ## - ADDITIONAL PHYSICAL FORM AVAILABLE NOTE | |
| Additional physical form available note | Issued also as CD |
| 653 #4 - INDEX TERM--UNCONTROLLED | |
| Uncontrolled term | Clotrimazole |
| 653 #4 - INDEX TERM--UNCONTROLLED | |
| Uncontrolled term | Imatinib |
| 653 #4 - INDEX TERM--UNCONTROLLED | |
| Uncontrolled term | T47D |
| 700 0# - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Nermin Abdelhamid Sadik , |
| Relator term | |
| 700 0# - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Samia Abdelsamiaa Shouman , |
| Relator term | |
| 700 0# - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Tarek Mohamed Kamal Motawi , |
| Relator term | |
| 856 ## - ELECTRONIC LOCATION AND ACCESS | |
| Uniform Resource Identifier | <a href="http://172.23.153.220/th.pdf">http://172.23.153.220/th.pdf</a> |
| 905 ## - LOCAL DATA ELEMENT E, LDE (RLIN) | |
| Cataloger | Nazla |
| Reviser | Revisor |
| 905 ## - LOCAL DATA ELEMENT E, LDE (RLIN) | |
| Cataloger | Soheir |
| Reviser | Cataloger |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) | |
| Source of classification or shelving scheme | Dewey Decimal Classification |
| Koha item type | Thesis |
| Source of classification or shelving scheme | Not for loan | Home library | Current library | Date acquired | Full call number | Barcode | Date last seen | Koha item type | Copy number |
|---|---|---|---|---|---|---|---|---|---|
| Dewey Decimal Classification | المكتبة المركزبة الجديدة - جامعة القاهرة | قاعة الرسائل الجامعية - الدور الاول | 11.02.2024 | Cai01.08.01.M.Sc.2015.Sa.E | 01010110068542000 | 22.09.2023 | Thesis | ||
| Dewey Decimal Classification | المكتبة المركزبة الجديدة - جامعة القاهرة | مخـــزن الرســائل الجـــامعية - البدروم | 11.02.2024 | Cai01.08.01.M.Sc.2015.Sa.E | 01020110068542000 | 22.09.2023 | CD - Rom | 68542.CD |