Bacteriological and molecular studies on listeria monocytogenes Isolated from Rabbits / (Record no. 56257)
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| 000 -LEADER | |
|---|---|
| fixed length control field | 03053cam a2200349 a 4500 |
| 003 - CONTROL NUMBER IDENTIFIER | |
| control field | EG-GiCUC |
| 005 - DATE AND TIME OF LATEST TRANSACTION | |
| control field | 20250223031502.0 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION | |
| fixed length control field | 160504s2015 ua do f m 000 0 eng d |
| 040 ## - CATALOGING SOURCE | |
| Original cataloging agency | EG-GiCUC |
| Language of cataloging | eng |
| Transcribing agency | EG-GiCUC |
| 041 0# - LANGUAGE CODE | |
| Language code of text/sound track or separate title | eng |
| 049 ## - LOCAL HOLDINGS (OCLC) | |
| Holding library | Deposite |
| 097 ## - Thesis Degree | |
| Thesis Level | M.Sc |
| 099 ## - LOCAL FREE-TEXT CALL NUMBER (OCLC) | |
| Classification number | Cai01.10.10.M.Sc.2015.Ma.B |
| 100 0# - MAIN ENTRY--PERSONAL NAME | |
| Personal name | Mahmoud Kamel Abdelfattah Kamel |
| 245 10 - TITLE STATEMENT | |
| Title | Bacteriological and molecular studies on listeria monocytogenes Isolated from Rabbits / |
| Statement of responsibility, etc. | Mahmoud Kamel Abdelfattah Kamel ; Supervised Khaled Farouk Mohamed , Sherif Abdelmonium Omar , Soad Abdelaziz Abdelwanis |
| 246 15 - VARYING FORM OF TITLE | |
| Title proper/short title | دراسات بكتريولوجية و جزيئية على الليستريا مونوسيتوجينس المعزولة من الارانب |
| 260 ## - PUBLICATION, DISTRIBUTION, ETC. | |
| Place of publication, distribution, etc. | Cairo : |
| Name of publisher, distributor, etc. | Mahmoud Kamel Abdelfattah Kamel , |
| Date of publication, distribution, etc. | 2015 |
| 300 ## - PHYSICAL DESCRIPTION | |
| Extent | 179 P. : |
| Other physical details | charts , photographs ; |
| Dimensions | 25cm |
| 502 ## - DISSERTATION NOTE | |
| Dissertation note | Thesis (M.Sc.) - Cairo University - Faculty of Veterinary Medicine - Department of Microbiology |
| 520 ## - SUMMARY, ETC. | |
| Summary, etc. | Listeria monocytogenes represent one of the most important pathogens that cause destructive economical losses in rabbit production, which also had gain public health concern as it can cause listeriosis in human. Due to the recent outbreaks, recalls and deaths associated with Listeria monocytogenes in rabbit farms affecting the rabbit production in Egypt. The objectives of the present study were to isolate, identificate L. monocytogenes using bacteriological examination and confirm the results by PCR and then detection of virulence genes (prfA, inlA, inlB and hlyA) in the isolated strains. One hundered samples were collected from diseased and apparently health rabbits. Out of 100 teseted samples, 32 samples were positive for L. monocytogenes by bacteriological examination, while 54 samples were positive using PCR. Where the best site for isolation of L. monocytogenes was from ear swabs (38.8%) then Liver (21.4%) but not detected in bone marrow using both techniques. Virulence gene in the 54 positive L. monocytogenes isolates were detected using selected 4 sets of primers for inlA, inlB, hylA and prfA genes, 26 samples were positive for inlA, 32 samples were positive for inlB, 31 samples were positive for hylA and 27 samples were positive for prfA by using PCR. Based on the study, PCR technique is more sensitive and rapid comparing to traditional bacteriological methods. In addition that virulence genes studies obtain accurate and specific identification and differentiation among Listeria spp. advanced studies should be applied to improve the efficiency of PCR technique for virulence study by complete gene sequence |
| 530 ## - ADDITIONAL PHYSICAL FORM AVAILABLE NOTE | |
| Additional physical form available note | Issued also as CD |
| 653 #4 - INDEX TERM--UNCONTROLLED | |
| Uncontrolled term | Isolation |
| 653 #4 - INDEX TERM--UNCONTROLLED | |
| Uncontrolled term | Listeria monomcytogenes |
| 653 #4 - INDEX TERM--UNCONTROLLED | |
| Uncontrolled term | Rabbit |
| 700 0# - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Khaled Farouk Mohamed , |
| Relator term | |
| 700 0# - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Sherif Abdelmonium Omar , |
| Relator term | |
| 700 0# - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Soad Abdelaziz Abdelwanis , |
| Relator term | |
| 856 ## - ELECTRONIC LOCATION AND ACCESS | |
| Uniform Resource Identifier | <a href="http://172.23.153.220/th.pdf">http://172.23.153.220/th.pdf</a> |
| 905 ## - LOCAL DATA ELEMENT E, LDE (RLIN) | |
| Cataloger | Nazla |
| Reviser | Revisor |
| 905 ## - LOCAL DATA ELEMENT E, LDE (RLIN) | |
| Cataloger | Samia |
| Reviser | Cataloger |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) | |
| Source of classification or shelving scheme | Dewey Decimal Classification |
| Koha item type | Thesis |
| Source of classification or shelving scheme | Not for loan | Home library | Current library | Date acquired | Full call number | Barcode | Date last seen | Koha item type | Copy number |
|---|---|---|---|---|---|---|---|---|---|
| Dewey Decimal Classification | المكتبة المركزبة الجديدة - جامعة القاهرة | قاعة الرسائل الجامعية - الدور الاول | 11.02.2024 | Cai01.10.10.M.Sc.2015.Ma.B | 01010110068749000 | 22.09.2023 | Thesis | ||
| Dewey Decimal Classification | المكتبة المركزبة الجديدة - جامعة القاهرة | مخـــزن الرســائل الجـــامعية - البدروم | 11.02.2024 | Cai01.10.10.M.Sc.2015.Ma.B | 01020110068749000 | 22.09.2023 | CD - Rom | 68749.CD |