Immunological studies on mycobacterium avium subsp. paratuberculosis for improving the immunodiagnosisof Johne'sdisease / (Record no. 56709)
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| 000 -LEADER | |
|---|---|
| fixed length control field | 02622cam a2200337 a 4500 |
| 003 - CONTROL NUMBER IDENTIFIER | |
| control field | EG-GiCUC |
| 005 - DATE AND TIME OF LATEST TRANSACTION | |
| control field | 20250223031516.0 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION | |
| fixed length control field | 160529s2015 ua h f m 000 0 eng d |
| 040 ## - CATALOGING SOURCE | |
| Original cataloging agency | EG-GiCUC |
| Language of cataloging | eng |
| Transcribing agency | EG-GiCUC |
| 041 0# - LANGUAGE CODE | |
| Language code of text/sound track or separate title | eng |
| 049 ## - LOCAL HOLDINGS (OCLC) | |
| Holding library | Deposite |
| 097 ## - Thesis Degree | |
| Thesis Level | Ph.D |
| 099 ## - LOCAL FREE-TEXT CALL NUMBER (OCLC) | |
| Classification number | Cai01.10.10.Ph.D.2015.Sh.I |
| 100 0# - MAIN ENTRY--PERSONAL NAME | |
| Personal name | Shaymaa Abdelmalek Mohammed |
| 245 10 - TITLE STATEMENT | |
| Title | Immunological studies on mycobacterium avium subsp. paratuberculosis for improving the immunodiagnosisof Johne'sdisease / |
| Statement of responsibility, etc. | Shaymaa Abdelmalek Mohammed ; Supervised Rafik Tawfik Mohammed Soliman , Khaled Farouk Mohammed Alamry |
| 246 15 - VARYING FORM OF TITLE | |
| Title proper/short title | دراسات مناعيةعلى ميكروب المايكوباكتيريام أفيام تحت نوع باراتيوبركيولوزيس لتطويرالتشخيص المعملى لمرض يونز |
| 260 ## - PUBLICATION, DISTRIBUTION, ETC. | |
| Place of publication, distribution, etc. | Cairo : |
| Name of publisher, distributor, etc. | Shaymaa Abdelmalek Mohammed , |
| Date of publication, distribution, etc. | 2015 |
| 300 ## - PHYSICAL DESCRIPTION | |
| Extent | 149 P. : |
| Other physical details | facsimiles ; |
| Dimensions | 25cm |
| 502 ## - DISSERTATION NOTE | |
| Dissertation note | Thesis (Ph.D.) - Cairo University - Faculty of Veterinary Medicine - Department of Microbiology |
| 520 ## - SUMMARY, ETC. | |
| Summary, etc. | Mycobacterium avium subsp. paratuberculosis (MAP) is the cause of Johne's disease (JD), a chronic granulomatous intestinal disease of cattle. The disease represents one of the important problemsfacing dairy industry andcausing high economic losses. 100 fecal and 100serum samples were collected from JD clinically suspected cases.Sixtyout of the 100 fecal samples werepositive usingZiehl Neelsen stain showing acid and alcohol fast red bacilli.. Bacteriological examination of the 100 fecal samples reveled the isolation of 34MAP isolates.The recovered MAPisolates were tested by conventional and nested PCR.The nested PCR provedthe identity of the 34 MAP isolates; however, the conventional PCR using different primers gave variable results. Using a commercialMAP specific ELISAkits, only 13 out of the 100 serum samples were positive. The recoveredMAP isolates were cultured on modified Middlebrook7H9 and modified Watson Reid liquid media for preparation of theMAP cultural filtrate (CF) proteins, which were then separated by SDS-PAGE.The MAPCF immunogenic determinants were identified using western-Blot technique. Different immunogenic bands from 9-72 kDa were identified |
| 530 ## - ADDITIONAL PHYSICAL FORM AVAILABLE NOTE | |
| Additional physical form available note | Issued also as CD |
| 653 #4 - INDEX TERM--UNCONTROLLED | |
| Uncontrolled term | Immunodiagnosis |
| 653 #4 - INDEX TERM--UNCONTROLLED | |
| Uncontrolled term | Mycobacterium avium subsp |
| 653 #4 - INDEX TERM--UNCONTROLLED | |
| Uncontrolled term | Paratuberculosis |
| 700 0# - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Khaled Farouk Mohamed Alamry , |
| Relator term | |
| 700 0# - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Rafik Tawfik Mohamed Soliman , |
| Relator term | |
| 856 ## - ELECTRONIC LOCATION AND ACCESS | |
| Uniform Resource Identifier | <a href="http://172.23.153.220/th.pdf">http://172.23.153.220/th.pdf</a> |
| 905 ## - LOCAL DATA ELEMENT E, LDE (RLIN) | |
| Cataloger | Aml |
| Reviser | Cataloger |
| 905 ## - LOCAL DATA ELEMENT E, LDE (RLIN) | |
| Cataloger | Nazla |
| Reviser | Revisor |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) | |
| Source of classification or shelving scheme | Dewey Decimal Classification |
| Koha item type | Thesis |
| Source of classification or shelving scheme | Not for loan | Home library | Current library | Date acquired | Full call number | Barcode | Date last seen | Koha item type | Copy number |
|---|---|---|---|---|---|---|---|---|---|
| Dewey Decimal Classification | المكتبة المركزبة الجديدة - جامعة القاهرة | قاعة الرسائل الجامعية - الدور الاول | 11.02.2024 | Cai01.10.10.Ph.D.2015.Sh.I | 01010110069087000 | 22.09.2023 | Thesis | ||
| Dewey Decimal Classification | المكتبة المركزبة الجديدة - جامعة القاهرة | مخـــزن الرســائل الجـــامعية - البدروم | 11.02.2024 | Cai01.10.10.Ph.D.2015.Sh.I | 01020110069087000 | 22.09.2023 | CD - Rom | 69087.CD |