Application of metal nanoparticles in treatment of tuberculosis / (Record no. 57019)
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| 000 -LEADER | |
|---|---|
| fixed length control field | 03340cam a2200349 a 4500 |
| 003 - CONTROL NUMBER IDENTIFIER | |
| control field | EG-GiCUC |
| 005 - DATE AND TIME OF LATEST TRANSACTION | |
| control field | 20250223031525.0 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION | |
| fixed length control field | 160625s2015 ua o f m 000 0 eng d |
| 040 ## - CATALOGING SOURCE | |
| Original cataloging agency | EG-GiCUC |
| Language of cataloging | eng |
| Transcribing agency | EG-GiCUC |
| 041 0# - LANGUAGE CODE | |
| Language code of text/sound track or separate title | eng |
| 049 ## - LOCAL HOLDINGS (OCLC) | |
| Holding library | Deposite |
| 097 ## - Thesis Degree | |
| Thesis Level | Ph.D |
| 099 ## - LOCAL FREE-TEXT CALL NUMBER (OCLC) | |
| Classification number | Cai01.10.10.Ph.D.2015.Ha.A |
| 100 0# - MAIN ENTRY--PERSONAL NAME | |
| Personal name | Hala Ragab Khalil Ali |
| 245 10 - TITLE STATEMENT | |
| Title | Application of metal nanoparticles in treatment of tuberculosis / |
| Statement of responsibility, etc. | Hala Ragab Khalil Ali ; Supervised Salah Eldin Abdelkarim Selim , Esam Amin Naser , Mostafa A. AboNorag |
| 246 15 - VARYING FORM OF TITLE | |
| Title proper/short title | إستخدام الجزيئات النانونية المعدنية فى علاج السل |
| 260 ## - PUBLICATION, DISTRIBUTION, ETC. | |
| Place of publication, distribution, etc. | Cairo : |
| Name of publisher, distributor, etc. | Hala Ragab Khalil Ali , |
| Date of publication, distribution, etc. | 2015 |
| 300 ## - PHYSICAL DESCRIPTION | |
| Extent | 145 P. : |
| Other physical details | photographs ; |
| Dimensions | 25cm |
| 502 ## - DISSERTATION NOTE | |
| Dissertation note | Thesis (Ph.D.) - Cairo University - Faculty of Veterinary Medicine - Department of Microbiology |
| 520 ## - SUMMARY, ETC. | |
| Summary, etc. | Development of drug resistant strains such as multi drug resistant TB (MDR-TB) and extensive drug resistant TB (EXDR-TB) threatens the progress made in TB controls and prompt the need to alternative strategies. The aim of this work was to investigate the anti-mycobacterial activity of two noble metal nanoparticles either in non-conjugated form or conjugated with rifampicin. Gold (spheres and rods) and silver (spheres) nanoparticles were synthesized and characterized using TEM and UV{u2013}vis spectroscopy. The anti-mycobacterial activity of silver nanospheres (AgNSs) was studied using the malachite green decolorisation assay (MGDA) and cultivation on Lowenstein Jensen media, and the results indicate that AgNSs have a strong anti-mycobacterial effect with 80 æg/ml MIC. To test the cytotoxic effect of gold and silver nanospheres on RAW264.7 cell line as a macrophage cell model, which is the host of TB, we did a comparative study using XTT assay and microscopic examination. Results indicated that silver nanospheres were severely toxic while the gold nanospheres were safer to murine macrophage RAW264.7 cell. Therefore, silver nanospheres were excluded from this work as its toxicity to host cells. Gold nanoparticles (spheres and rods) were conjugated with rifampicin and the successful conjugation was confirmed with UV{u2013}vis spectroscopy. Then, the anti-mycobacterial activity of conjugated and non-conjugated AuNPs with rifampicin against extracellular TB were assayed using the malachite green decolorisation assay (MGDA) and cultivation on Lowenstein Jensen media and Middlebrook agar, the results revealed that the shapes of gold nanoparticles played an important role in the anti-mycobacterial activity, as the rods have a strong anti-mycobacterial activity while the spheres have no anti-mycobacterial effect. The impact of the cell types (RAW264.7 macrophage cell line, HSC-3 human oral squamous cell carcinoma and MCF7 human breast adenocarcinoma cells) on the gold nanoparticle uptake was studied using the dark field scattering microscopy |
| 530 ## - ADDITIONAL PHYSICAL FORM AVAILABLE NOTE | |
| Additional physical form available note | Issued also as CD |
| 653 #4 - INDEX TERM--UNCONTROLLED | |
| Uncontrolled term | AgNSs |
| 653 #4 - INDEX TERM--UNCONTROLLED | |
| Uncontrolled term | AuNPs MGDA |
| 653 #4 - INDEX TERM--UNCONTROLLED | |
| Uncontrolled term | TEM |
| 700 0# - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Esam Amin Naser , |
| Relator term | |
| 700 0# - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Mostafa Ahmed AboNorag , |
| Relator term | |
| 700 0# - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Salah Eldin Abdelkarim Selim , |
| Relator term | |
| 856 ## - ELECTRONIC LOCATION AND ACCESS | |
| Uniform Resource Identifier | <a href="http://172.23.153.220/th.pdf">http://172.23.153.220/th.pdf</a> |
| 905 ## - LOCAL DATA ELEMENT E, LDE (RLIN) | |
| Cataloger | Nazla |
| Reviser | Revisor |
| 905 ## - LOCAL DATA ELEMENT E, LDE (RLIN) | |
| Cataloger | Samia |
| Reviser | Cataloger |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) | |
| Source of classification or shelving scheme | Dewey Decimal Classification |
| Koha item type | Thesis |
| Source of classification or shelving scheme | Not for loan | Home library | Current library | Date acquired | Full call number | Barcode | Date last seen | Koha item type | Copy number |
|---|---|---|---|---|---|---|---|---|---|
| Dewey Decimal Classification | المكتبة المركزبة الجديدة - جامعة القاهرة | قاعة الرسائل الجامعية - الدور الاول | 11.02.2024 | Cai01.10.10.Ph.D.2015.Ha.A | 01010110069356000 | 22.09.2023 | Thesis | ||
| Dewey Decimal Classification | المكتبة المركزبة الجديدة - جامعة القاهرة | مخـــزن الرســائل الجـــامعية - البدروم | 11.02.2024 | Cai01.10.10.Ph.D.2015.Ha.A | 01020110069356000 | 22.09.2023 | CD - Rom | 69356.CD |