Studying virus resistance induction against bipartite begomovirus via gene silencing / (Record no. 58612)
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| 000 -LEADER | |
|---|---|
| fixed length control field | 02615cam a2200349 a 4500 |
| 003 - CONTROL NUMBER IDENTIFIER | |
| control field | EG-GiCUC |
| 005 - DATE AND TIME OF LATEST TRANSACTION | |
| control field | 20250223031613.0 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION | |
| fixed length control field | 161114s2016 ua o f m 000 0 eng d |
| 040 ## - CATALOGING SOURCE | |
| Original cataloging agency | EG-GiCUC |
| Language of cataloging | eng |
| Transcribing agency | EG-GiCUC |
| 041 0# - LANGUAGE CODE | |
| Language code of text/sound track or separate title | eng |
| 049 ## - LOCAL HOLDINGS (OCLC) | |
| Holding library | Deposite |
| 097 ## - Thesis Degree | |
| Thesis Level | M.Sc |
| 099 ## - LOCAL FREE-TEXT CALL NUMBER (OCLC) | |
| Classification number | Cai01.07.10.M.Sc.2016.Om.S |
| 100 0# - MAIN ENTRY--PERSONAL NAME | |
| Personal name | Omnia Taha Hussein Mohamed |
| 245 10 - TITLE STATEMENT | |
| Title | Studying virus resistance induction against bipartite begomovirus via gene silencing / |
| Statement of responsibility, etc. | Omnia Taha Hussein Mohamed ; Supervised Naglaa Abdalmoneim Abdallah , Abdelhadi Abdallah Abdelhadi , Inas Farouk Fahmy |
| 246 15 - VARYING FORM OF TITLE | |
| Title proper/short title | ثنائيه الوحدات بواسطة الكبت الجينى لبعض جينات الفيروس Begomovirus دراسة على المقاومه الفيروسيه المستحدثه ضد |
| 260 ## - PUBLICATION, DISTRIBUTION, ETC. | |
| Place of publication, distribution, etc. | Cairo : |
| Name of publisher, distributor, etc. | Omnia Taha Hussein Mohamed , |
| Date of publication, distribution, etc. | 2016 |
| 300 ## - PHYSICAL DESCRIPTION | |
| Extent | 104 P. : |
| Other physical details | photographs ; |
| Dimensions | 25cm |
| 502 ## - DISSERTATION NOTE | |
| Dissertation note | Thesis (M.Sc.) - Cairo University - Faculty of Agriculture - Department of Genetics |
| 520 ## - SUMMARY, ETC. | |
| Summary, etc. | Squash leaf curl virus (SqLCV) is a bipartite begomovirus affecting squash plants. In the last decade, SqlCV has been widely spread in the Middle East and caused huge yield loss in Egypt. It transmited by whitefly bemisia tabaci biotype B causing severe leaf curling, vein banding, molting ending by stunting as a late symptom. In this study posttranscriptional gene silencing (PTGS) has been induced to develop virus resistance. Two gene fragments from SqLCV were introduced in sense and antisense orientations using pFGC5941 vector to be expressed as a hairpin RNA (hpRNA). The first fragment was 348 bp at the 3' end of the replication associated protein gene (Rep or AC1), which had 50 bp overlapping with the transcription activation protein gene (TrAP or AC2). The second fragment was 879 bp representing the full sequence of the movement protein gene (BC1). Agrobacterium LB4404 was used in transient expression employing the syringe spotted technique in squash plants (cucurbita pepo L. cv. eskandarani). The infilterated plants harbouring the construct containing the Rep/TrAP fragment proved to be the best region for initiating PTGS |
| 530 ## - ADDITIONAL PHYSICAL FORM AVAILABLE NOTE | |
| Additional physical form available note | Issued also as CD |
| 653 #4 - INDEX TERM--UNCONTROLLED | |
| Uncontrolled term | Post transcriptional gene silencing |
| 653 #4 - INDEX TERM--UNCONTROLLED | |
| Uncontrolled term | Small interfering RNA |
| 653 #4 - INDEX TERM--UNCONTROLLED | |
| Uncontrolled term | Squash leaf curl virus |
| 700 0# - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Abdelhadi Abdallah Abdelhadi , |
| Relator term | |
| 700 0# - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Inas Farouk Fahmy , |
| Relator term | |
| 700 0# - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Naglaa Abdalmoneim Abdallah , |
| Relator term | |
| 856 ## - ELECTRONIC LOCATION AND ACCESS | |
| Uniform Resource Identifier | <a href="http://172.23.153.220/th.pdf">http://172.23.153.220/th.pdf</a> |
| 905 ## - LOCAL DATA ELEMENT E, LDE (RLIN) | |
| Cataloger | Nazla |
| Reviser | Revisor |
| 905 ## - LOCAL DATA ELEMENT E, LDE (RLIN) | |
| Cataloger | Samia |
| Reviser | Cataloger |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) | |
| Source of classification or shelving scheme | Dewey Decimal Classification |
| Koha item type | Thesis |
| Source of classification or shelving scheme | Not for loan | Home library | Current library | Date acquired | Full call number | Barcode | Date last seen | Koha item type | Copy number |
|---|---|---|---|---|---|---|---|---|---|
| Dewey Decimal Classification | المكتبة المركزبة الجديدة - جامعة القاهرة | قاعة الرسائل الجامعية - الدور الاول | 11.02.2024 | Cai01.07.10.M.Sc.2016.Om.S | 01010110070240000 | 22.09.2023 | Thesis | ||
| Dewey Decimal Classification | المكتبة المركزبة الجديدة - جامعة القاهرة | مخـــزن الرســائل الجـــامعية - البدروم | 11.02.2024 | Cai01.07.10.M.Sc.2016.Om.S | 01020110070240000 | 22.09.2023 | CD - Rom | 70240.CD |