Application of molecular biological techniques for detection of mycotoxigenic fungi and characterization of genes responsible for toxin production / (Record no. 58826)
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| 000 -LEADER | |
|---|---|
| fixed length control field | 03355cam a2200349 a 4500 |
| 003 - CONTROL NUMBER IDENTIFIER | |
| control field | EG-GiCUC |
| 005 - DATE AND TIME OF LATEST TRANSACTION | |
| control field | 20250223031620.0 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION | |
| fixed length control field | 161129s2016 ua dh f m 000 0 eng d |
| 040 ## - CATALOGING SOURCE | |
| Original cataloging agency | EG-GiCUC |
| Language of cataloging | eng |
| Transcribing agency | EG-GiCUC |
| 041 0# - LANGUAGE CODE | |
| Language code of text/sound track or separate title | eng |
| 049 ## - LOCAL HOLDINGS (OCLC) | |
| Holding library | Deposite |
| 097 ## - Thesis Degree | |
| Thesis Level | Ph.D |
| 099 ## - LOCAL FREE-TEXT CALL NUMBER (OCLC) | |
| Classification number | Cai01.10.10.Ph.D.2016.Ah.A |
| 100 0# - MAIN ENTRY--PERSONAL NAME | |
| Personal name | Ahmed Mohamed Attia Elhamaky |
| 245 10 - TITLE STATEMENT | |
| Title | Application of molecular biological techniques for detection of mycotoxigenic fungi and characterization of genes responsible for toxin production / |
| Statement of responsibility, etc. | Ahmed Mohamed Attia Elhamaky ; Supervised Heidy Mohamed Shawky , Mohamed Kamal Refai , Atef Abdelaziz Hassan |
| 246 15 - VARYING FORM OF TITLE | |
| Title proper/short title | استخدام طرق البيولوجيا الجزيئية في الكشف عن الفطريات المفرزة للسموم وتحديد الجينات المسئولة عن افراز تلك السموم |
| 260 ## - PUBLICATION, DISTRIBUTION, ETC. | |
| Place of publication, distribution, etc. | Cairo : |
| Name of publisher, distributor, etc. | Ahmed Mohamed Attia Elhamaky , |
| Date of publication, distribution, etc. | 2016 |
| 300 ## - PHYSICAL DESCRIPTION | |
| Extent | 219 P. : |
| Other physical details | charts , facsimiles ; |
| Dimensions | 25cm |
| 502 ## - DISSERTATION NOTE | |
| Dissertation note | Thesis (Ph.D.) - Cairo University - Faculty of Veterinary Medicine - Department of Microbiology |
| 520 ## - SUMMARY, ETC. | |
| Summary, etc. | A total of one hundred feed samples were collected from Cairo and Giza Governorates and screened for aflatoxin producing and ochratoxin producing fungal isolates. A total of 106 fungal isolate comprising, Aspergillus flavus, A. ochraceus and A. niger were recovered from feed samples and tested for aflatoxins and ochratoxin A (OTA) production. The most predominant isolate was A. flavus, which was recovered at the range of (40-55%), followed by A. niger (30-50 %) and A. ochraceus (15-20%). Thirty three of 47 A. flavus isolates produced aflatoxin B1 and B2 at an average levels of (170-750 ppb), while, 22 of 44 tested isolates of A. niger produced OTA with an average levels of (100-550 ppb), whereas 12 of 15 A. ochraceus isolates produced OTA at average levels of (300-700 ppb). Molecular identification of 16 toxigenic fungal isolates (5 A. flavus, 6 A. ochraceus and 5 A. niger) was carried out by PCR. The results of PCR of the DNA extracted from these isolates using ITS primer confirmed the identification of A. flavus, A. ochraceous and A. niger. The application of real-time PCR (RT-PCR) system directed against the nor-1 gene of the aflatoxins biosynthetic pathway was applied on the DNA extracted from the 5 selected strains of A. flavus. The amplification plot of the DNA samples indicated the presence of nor-1 gene in all aflatoxins-producing A. flavus isolates and in only one isolate of the negative aflatoxins-producing A. flavus. On the other hand, the use of primer set for amplification of omtB gene responsible for AF production amplified 611 bp fragments bands in all aflatoxigenic A. flavus, while, no band was detected in all negative aflatoxigenic isolates. All the sequenced A. flavus isolates were confirmed to belong to A. flavus species and were 100% similar to the reference isolates of A. flavus |
| 530 ## - ADDITIONAL PHYSICAL FORM AVAILABLE NOTE | |
| Additional physical form available note | Issued also as CD |
| 653 #4 - INDEX TERM--UNCONTROLLED | |
| Uncontrolled term | Ochratoxigenic |
| 653 #4 - INDEX TERM--UNCONTROLLED | |
| Uncontrolled term | PCR |
| 653 #4 - INDEX TERM--UNCONTROLLED | |
| Uncontrolled term | Sequencing |
| 700 0# - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Atef Abdelaziz Hassan , |
| Relator term | |
| 700 0# - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Heidy Mohamed Shawky Aboelyazeed , |
| Relator term | |
| 700 0# - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Mohamed Kamal Refai , |
| Relator term | |
| 856 ## - ELECTRONIC LOCATION AND ACCESS | |
| Uniform Resource Identifier | <a href="http://172.23.153.220/th.pdf">http://172.23.153.220/th.pdf</a> |
| 905 ## - LOCAL DATA ELEMENT E, LDE (RLIN) | |
| Cataloger | Nazla |
| Reviser | Revisor |
| 905 ## - LOCAL DATA ELEMENT E, LDE (RLIN) | |
| Cataloger | Soheir |
| Reviser | Cataloger |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) | |
| Source of classification or shelving scheme | Dewey Decimal Classification |
| Koha item type | Thesis |
| Source of classification or shelving scheme | Not for loan | Home library | Current library | Date acquired | Full call number | Barcode | Date last seen | Koha item type | Copy number |
|---|---|---|---|---|---|---|---|---|---|
| Dewey Decimal Classification | المكتبة المركزبة الجديدة - جامعة القاهرة | قاعة الرسائل الجامعية - الدور الاول | 11.02.2024 | Cai01.10.10.Ph.D.2016.Ah.A | 01010110070393000 | 22.09.2023 | Thesis | ||
| Dewey Decimal Classification | المكتبة المركزبة الجديدة - جامعة القاهرة | مخـــزن الرســائل الجـــامعية - البدروم | 11.02.2024 | Cai01.10.10.Ph.D.2016.Ah.A | 01020110070393000 | 22.09.2023 | CD - Rom | 70393.CD |