Preparation of saponin-treated vaccine from NDV genotype chinese 7d / (Record no. 63136)
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| 000 -LEADER | |
|---|---|
| fixed length control field | 03050cam a2200349 a 4500 |
| 003 - CONTROL NUMBER IDENTIFIER | |
| control field | EG-GiCUC |
| 005 - DATE AND TIME OF LATEST TRANSACTION | |
| control field | 20250223031831.0 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION | |
| fixed length control field | 171024s2017 ua f m 000 0 eng d |
| 040 ## - CATALOGING SOURCE | |
| Original cataloging agency | EG-GiCUC |
| Language of cataloging | eng |
| Transcribing agency | EG-GiCUC |
| 041 0# - LANGUAGE CODE | |
| Language code of text/sound track or separate title | eng |
| 049 ## - LOCAL HOLDINGS (OCLC) | |
| Holding library | Deposite |
| 097 ## - Thesis Degree | |
| Thesis Level | Ph.D |
| 099 ## - LOCAL FREE-TEXT CALL NUMBER (OCLC) | |
| Classification number | Cai01.10.17.Ph.D.2017.Wa.P |
| 100 0# - MAIN ENTRY--PERSONAL NAME | |
| Personal name | Wahid Hussein Mahmoud Eldabae |
| 245 10 - TITLE STATEMENT | |
| Title | Preparation of saponin-treated vaccine from NDV genotype chinese 7d / |
| Statement of responsibility, etc. | Wahid Hussein Mahmoud Eldabae ; Supervised Hussein Aly Hussein , Ismail Mohamed Reda , Nagwa Sayed Ata |
| 246 15 - VARYING FORM OF TITLE | |
| Title proper/short title | تحضير لقاح معالج بمادة الصابونين من فيروس مرض النيوكاسل النوع الوراثى الصينى 7د |
| 260 ## - PUBLICATION, DISTRIBUTION, ETC. | |
| Place of publication, distribution, etc. | Cairo : |
| Name of publisher, distributor, etc. | Wahid Hussein Mahmoud Eldabae , |
| Date of publication, distribution, etc. | 2017 |
| 300 ## - PHYSICAL DESCRIPTION | |
| Extent | 177 P. ; |
| Dimensions | 25cm |
| 502 ## - DISSERTATION NOTE | |
| Dissertation note | Thesis (Ph.D.) - Cairo University - Faculty of Veterinary Medicine - Department of Virology |
| 520 ## - SUMMARY, ETC. | |
| Summary, etc. | In the present study, chemical attenuation was carried out for local NDV genotype vIId designated as NDV-F278-RLQP-CH-EG circulating in Egypt. The virus was propagated in 9-11 day SPF eggs via allantoic cavity and was serially passaged for 35 passages. Then, nitrous acid was used for attenuation and the treated virus was inoculated into SPF eggs. After incubation at 26{u00B0}C for 4-6 days, the harvested allantoic fluid revealed negative hemagglutination even after five passages of such harvest. Assaying of the allantoic fluid harvested from fifth passage of treated virus by real time RT-PCR revealed positive result indicating that nitrous acid affects the HA property of the virus. Inoculation of treated virus in SPF chickens, mortalities developed along ten days observation period and HA property of the virus was recovered again following isolation in SPF eggs indicating failure of attenuation. Therefore, second cycle of treatment using saponin was applied on the propagated virus after 35 passages. Saponin was used to treat virus at different times 10,20,30,45 and 60 minutes then, inoculated into SPF eggs and incubated at 37{u00B0}C for 3-5 days. The harvested allantoic fluid revealed positive hemagglutination. When treated virus incubated with saponin for 30 to 60 minutes inoculated in SPF chickens no clinical signs or mortalities were appeared. Challenge trial of different chicken groups with the virulent NDV genotype vIId designated as NDV-B7-RLQP-CH-EG-12 revealed 100%, 90% and 90% protection at times 30, 45 and 60 minutes respectively; indicating that the reaction of virus with saponin for 30 minutes was the best time to be used in vaccine preparation. |
| 530 ## - ADDITIONAL PHYSICAL FORM AVAILABLE NOTE | |
| Additional physical form available note | Issued also as CD |
| 653 #4 - INDEX TERM--UNCONTROLLED | |
| Uncontrolled term | Chemical attenuation |
| 653 #4 - INDEX TERM--UNCONTROLLED | |
| Uncontrolled term | NDV genotype VIId |
| 653 #4 - INDEX TERM--UNCONTROLLED | |
| Uncontrolled term | Saponin and chicken experiment |
| 700 0# - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Hussein Aly Hussein , |
| Relator term | |
| 700 0# - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Ismail Mohamed Reda , |
| Relator term | |
| 700 0# - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Nagwa Sayed Ata , |
| Relator term | |
| 856 ## - ELECTRONIC LOCATION AND ACCESS | |
| Uniform Resource Identifier | <a href="http://172.23.153.220/th.pdf">http://172.23.153.220/th.pdf</a> |
| 905 ## - LOCAL DATA ELEMENT E, LDE (RLIN) | |
| Cataloger | Nazla |
| Reviser | Revisor |
| 905 ## - LOCAL DATA ELEMENT E, LDE (RLIN) | |
| Cataloger | Samia |
| Reviser | Cataloger |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) | |
| Source of classification or shelving scheme | Dewey Decimal Classification |
| Koha item type | Thesis |
| Source of classification or shelving scheme | Not for loan | Home library | Current library | Date acquired | Full call number | Barcode | Date last seen | Koha item type | Copy number |
|---|---|---|---|---|---|---|---|---|---|
| Dewey Decimal Classification | المكتبة المركزبة الجديدة - جامعة القاهرة | قاعة الرسائل الجامعية - الدور الاول | 11.02.2024 | Cai01.10.17.Ph.D.2017.Wa.P | 01010110073160000 | 22.09.2023 | Thesis | ||
| Dewey Decimal Classification | المكتبة المركزبة الجديدة - جامعة القاهرة | مخـــزن الرســائل الجـــامعية - البدروم | 11.02.2024 | Cai01.10.17.Ph.D.2017.Wa.P | 01020110073160000 | 22.09.2023 | CD - Rom | 73160.CD |