Molecular genetics studies on olives / (Record no. 64193)

MARC details
000 -LEADER
fixed length control field 03322cam a2200349 a 4500
003 - CONTROL NUMBER IDENTIFIER
control field EG-GiCUC
005 - DATE AND TIME OF LATEST TRANSACTION
control field 20250223031902.0
008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION
fixed length control field 171223s2017 ua d f m 000 0 eng d
040 ## - CATALOGING SOURCE
Original cataloging agency EG-GiCUC
Language of cataloging eng
Transcribing agency EG-GiCUC
041 0# - LANGUAGE CODE
Language code of text/sound track or separate title eng
049 ## - LOCAL HOLDINGS (OCLC)
Holding library Deposite
097 ## - Thesis Degree
Thesis Level M.Sc
099 ## - LOCAL FREE-TEXT CALL NUMBER (OCLC)
Classification number Cai01.07.10.M.Sc.2017.Al.M
100 0# - MAIN ENTRY--PERSONAL NAME
Personal name Alsamman Mahmoud Mohammed Ali Alsamman
245 10 - TITLE STATEMENT
Title Molecular genetics studies on olives /
Statement of responsibility, etc. Alsamman Mahmoud Mohammed Ali Alsamman ; Supervised Ebtissam Hussein Aly Hussein , Basita Abbas Hussein , Sami Said Adawy
246 15 - VARYING FORM OF TITLE
Title proper/short title دراسات وراثية جزيئية علي الزيتون
260 ## - PUBLICATION, DISTRIBUTION, ETC.
Place of publication, distribution, etc. Cairo :
Name of publisher, distributor, etc. Alsamman Mahmoud Mohammed Ali Alsamman ,
Date of publication, distribution, etc. 2017
300 ## - PHYSICAL DESCRIPTION
Extent 126 P. :
Other physical details charts ;
Dimensions 25cm
502 ## - DISSERTATION NOTE
Dissertation note Thesis (M.Sc.) - Cairo University - Faculty of Agriculture- Department of Genetics
520 ## - SUMMARY, ETC.
Summary, etc. The genetic diversity among ten olive (Olea europaea) varieties was assessed using three molecular markers systems i.e., RAPD, SCoT and SAMPL. In addition, a new type of markers namely Selective Amplification of Start Codon Polymorphic Loci (SASPL) marker was developed. SASPL was validated in vitro by testing the genetic diversity among the ten olive varieties and the results were compared with that of RAPD, SCoT and SAMPL. Assesment also included primer selectivity, genome coverage and the ability to target genic regions using in silico PCR analysis. Finally, selected candidate PCR amplicons were sequenced to investigate the possibility to identify anonymous genes of olive. Eight SASPL primers were compared to 24 RAPD, 39 SCoT and 12 SAMPL primers. The total number of amplicons (TA) produced by the RAPD, SCoT, SAMPL and the eight SASPL primers were 359, 642, 571 and 269 amplicons, respectively. SAMPL revealed the highest average number of TA (47.6), average of polymorphic amplicons (PA) (18.1) and genetic similarity (GS) (96%) among the olive varieties. On the other hand, SASPL analysis provided higher average number of TA (33.6), average PA (16.2) and GS (93%) than SCoT and RAPD. The highest average of polymorphic information content (PIC) (0.2909) was exhibited by SASPL and the lowest average (0.2038) was revealed by SCoT. The highest number of unique bands (UB (111) was revealed by SCoT and the lowest UB (43) was obtained by SASPL. Across the four marker types, variety Maraki was characterized by the highest number of unique markers (74). Meanwhile, the variety Manzanillo was characterized by the lowest number of unique markers (8). In addition, in the in silico analysis SASPL exhibited the highest chromosomal coverage (0.59%) and targeted genes (1090) using the lowest number of primers. Additionally, the average area covered by the SASPL primers (354kb) was larger than SCoT and SAMPL. RAPD analysis provided the lowest potential, chromosomal coverage (0.04%) and number of targeted genes (17) compared to SASPL, SCoT and SAMPL analysis
530 ## - ADDITIONAL PHYSICAL FORM AVAILABLE NOTE
Additional physical form available note Issued also as CD
653 #4 - INDEX TERM--UNCONTROLLED
Uncontrolled term Olive
653 #4 - INDEX TERM--UNCONTROLLED
Uncontrolled term RAPD
653 #4 - INDEX TERM--UNCONTROLLED
Uncontrolled term SCoT
700 0# - ADDED ENTRY--PERSONAL NAME
Personal name Basita Abbas Hussein ,
Relator term
700 0# - ADDED ENTRY--PERSONAL NAME
Personal name Ebtissam Hussein Aly Hussein ,
Relator term
700 0# - ADDED ENTRY--PERSONAL NAME
Personal name Sami Said Adawy ,
Relator term
856 ## - ELECTRONIC LOCATION AND ACCESS
Uniform Resource Identifier <a href="http://172.23.153.220/th.pdf">http://172.23.153.220/th.pdf</a>
905 ## - LOCAL DATA ELEMENT E, LDE (RLIN)
Cataloger Nazla
Reviser Revisor
905 ## - LOCAL DATA ELEMENT E, LDE (RLIN)
Cataloger Shimaa
Reviser Cataloger
942 ## - ADDED ENTRY ELEMENTS (KOHA)
Source of classification or shelving scheme Dewey Decimal Classification
Koha item type Thesis
Holdings
Source of classification or shelving scheme Not for loan Home library Current library Date acquired Full call number Barcode Date last seen Koha item type Copy number
Dewey Decimal Classification   المكتبة المركزبة الجديدة - جامعة القاهرة قاعة الرسائل الجامعية - الدور الاول 11.02.2024 Cai01.07.10.M.Sc.2017.Al.M 01010110073872000 22.09.2023 Thesis  
Dewey Decimal Classification   المكتبة المركزبة الجديدة - جامعة القاهرة مخـــزن الرســائل الجـــامعية - البدروم 11.02.2024 Cai01.07.10.M.Sc.2017.Al.M 01020110073872000 22.09.2023 CD - Rom 73872.CD
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