Cloning and expression of BHV-1 outer surface protein genes / (Record no. 67383)
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| 000 -LEADER | |
|---|---|
| fixed length control field | 02775cam a2200337 a 4500 |
| 003 - CONTROL NUMBER IDENTIFIER | |
| control field | EG-GiCUC |
| 005 - DATE AND TIME OF LATEST TRANSACTION | |
| control field | 20250223032041.0 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION | |
| fixed length control field | 180909s2018 ua o f m 000 0 eng d |
| 040 ## - CATALOGING SOURCE | |
| Original cataloging agency | EG-GiCUC |
| Language of cataloging | eng |
| Transcribing agency | EG-GiCUC |
| 041 0# - LANGUAGE CODE | |
| Language code of text/sound track or separate title | eng |
| 049 ## - LOCAL HOLDINGS (OCLC) | |
| Holding library | Deposite |
| 097 ## - Thesis Degree | |
| Thesis Level | Ph.D |
| 099 ## - LOCAL FREE-TEXT CALL NUMBER (OCLC) | |
| Classification number | Cai01.10.17.Ph.D.2018.Em.C |
| 100 0# - MAIN ENTRY--PERSONAL NAME | |
| Personal name | Eman Reda Mohamed Abdo |
| 245 10 - TITLE STATEMENT | |
| Title | Cloning and expression of BHV-1 outer surface protein genes / |
| Statement of responsibility, etc. | Eman Reda Mohamed Abdo ; Supervised Hussein Aly Hussein , Alaa Abdelmoneim Ahmed Elkholy |
| 246 15 - VARYING FORM OF TITLE | |
| Title proper/short title | كلونة و التعبير عن جينات بروتينات السطح الخارجى لفيروس الهيربس البقرى نوع-1 |
| 260 ## - PUBLICATION, DISTRIBUTION, ETC. | |
| Place of publication, distribution, etc. | Cairo : |
| Name of publisher, distributor, etc. | Eman Reda Mohamed Abdo , |
| Date of publication, distribution, etc. | 2018 |
| 300 ## - PHYSICAL DESCRIPTION | |
| Extent | 136 P. : |
| Other physical details | photographs ; |
| Dimensions | 25cm |
| 502 ## - DISSERTATION NOTE | |
| Dissertation note | Thesis (Ph.D.) - Cairo University - Faculty of Veterinary Medicine - Department of Virology |
| 520 ## - SUMMARY, ETC. | |
| Summary, etc. | The glycoprotein D (gD) gene of bovine herpesvirus-1.1 Egyptian strain 2Abu-Hammad3 was cloned and expressed in spodopetra Frugiperda (Sf9) insect cells using baculovirus expression system. Full length gD encoding sequence was amplified by polymerase chain reaction (PCR) and cloned into the baculovirus shuttle vector; pMelBac B. The cloned gene was inserted in the genome of autographa california nuclear polyhydrosis virus (AcMNPV) under control of the polyhedrin promoter, through homologous recombination between the recombinant pMel/gD and a linearized triple cut baculovirus DNA (Bac-N-Blue) using liposome mediated transfection on to Sf9 cells. Recombinant baculovirus was selectively purified by plaque assay and verified for integrity of gD gene of BoHV-1.1 using PCR. The recombinant gD protein maintained their antigenic properties as determined by its reactivity with anti BoHV-1 positive serum using situ immunofluorescent and Western blot assays. The secreted recombinant gD in culture medium of infected insect cells was used as a coating antigen in an indirect enzyme linked immunosorbent assay (ELISA) to test its utility for detection of antibody against gD of BoHV-1. This ELISA was compared to standard virus neutralization test for detection of anti-BoHV-1antibody in a panal of bovine sera demonstrating closely correlated antibody titers in both. The developed indirect gD-ELISA was a reliable candidate for detection and validation of BoHV-1seropositive animals with high speci{uFB01}city |
| 530 ## - ADDITIONAL PHYSICAL FORM AVAILABLE NOTE | |
| Additional physical form available note | Issued also as CD |
| 653 #4 - INDEX TERM--UNCONTROLLED | |
| Uncontrolled term | Baculovirus |
| 653 #4 - INDEX TERM--UNCONTROLLED | |
| Uncontrolled term | BoHV-1 |
| 653 #4 - INDEX TERM--UNCONTROLLED | |
| Uncontrolled term | Glycoprotein D |
| 700 0# - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Alaa Abdelmoneim Ahmed Elkholy , |
| Relator term | |
| 700 0# - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Hussein Aly Hussein , |
| Relator term | |
| 856 ## - ELECTRONIC LOCATION AND ACCESS | |
| Uniform Resource Identifier | <a href="http://172.23.153.220/th.pdf">http://172.23.153.220/th.pdf</a> |
| 905 ## - LOCAL DATA ELEMENT E, LDE (RLIN) | |
| Cataloger | Nazla |
| Reviser | Revisor |
| 905 ## - LOCAL DATA ELEMENT E, LDE (RLIN) | |
| Cataloger | Samia |
| Reviser | Cataloger |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) | |
| Source of classification or shelving scheme | Dewey Decimal Classification |
| Koha item type | Thesis |
| Source of classification or shelving scheme | Not for loan | Home library | Current library | Date acquired | Full call number | Barcode | Date last seen | Koha item type | Copy number |
|---|---|---|---|---|---|---|---|---|---|
| Dewey Decimal Classification | المكتبة المركزبة الجديدة - جامعة القاهرة | قاعة الرسائل الجامعية - الدور الاول | 11.02.2024 | Cai01.10.17.Ph.D.2018.Em.C | 01010110075773000 | 22.09.2023 | Thesis | ||
| Dewey Decimal Classification | المكتبة المركزبة الجديدة - جامعة القاهرة | مخـــزن الرســائل الجـــامعية - البدروم | 11.02.2024 | Cai01.10.17.Ph.D.2018.Em.C | 01020110075773000 | 22.09.2023 | CD - Rom | 75773.CD |