Investigating the distribution and potential function of members of the Mmp-protein superfamily in escherichia coli / (Record no. 67454)
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| 000 -LEADER | |
|---|---|
| fixed length control field | 03174cam a2200349 a 4500 |
| 003 - CONTROL NUMBER IDENTIFIER | |
| control field | EG-GiCUC |
| 005 - DATE AND TIME OF LATEST TRANSACTION | |
| control field | 20250223032043.0 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION | |
| fixed length control field | 180913s2018 ua dh f m 000 0 eng d |
| 040 ## - CATALOGING SOURCE | |
| Original cataloging agency | EG-GiCUC |
| Language of cataloging | eng |
| Transcribing agency | EG-GiCUC |
| 041 0# - LANGUAGE CODE | |
| Language code of text/sound track or separate title | eng |
| 049 ## - LOCAL HOLDINGS (OCLC) | |
| Holding library | Deposite |
| 097 ## - Thesis Degree | |
| Thesis Level | M.Sc |
| 099 ## - LOCAL FREE-TEXT CALL NUMBER (OCLC) | |
| Classification number | Cai01.08.06.M.Sc.2018.Sa.I |
| 100 0# - MAIN ENTRY--PERSONAL NAME | |
| Personal name | Salma Hussein Abdelfattah |
| 245 10 - TITLE STATEMENT | |
| Title | Investigating the distribution and potential function of members of the Mmp-protein superfamily in escherichia coli / |
| Statement of responsibility, etc. | Salma Hussein Abdelfattah ; Supervised Mohamed Abdelaziz Toema , Ramy Karam Aziz , Reham Samir Mohamed |
| 246 15 - VARYING FORM OF TITLE | |
| Title proper/short title | و دراسة وظائفها المحتملة Escherichia coli فى بكتيريا الإشيريشيا القولونية MmpL استقصاء توزيع بروتينات عائلة |
| 260 ## - PUBLICATION, DISTRIBUTION, ETC. | |
| Place of publication, distribution, etc. | Cairo : |
| Name of publisher, distributor, etc. | Salma Hussein Abdelfattah , |
| Date of publication, distribution, etc. | 2018 |
| 300 ## - PHYSICAL DESCRIPTION | |
| Extent | 150 P. : |
| Other physical details | charts , facsimiles ; |
| Dimensions | 25cm |
| 502 ## - DISSERTATION NOTE | |
| Dissertation note | Thesis (M.Sc.) - Cairo University - Faculty of Pharmacy - Department of Microbiology and Immunology |
| 520 ## - SUMMARY, ETC. | |
| Summary, etc. | Escherichia coli is a beneficial common resident of the intestine, but a few strains can be pathogenic, e.g.E. coli O157:H7, which causes serious diseases. Because of E. coli{u2019}s versatility and genome plasticity, the bacteria have alarmingly developed resistance against many commonly used antibiotics by different mechanisms. Although E. coli has been extensively studied, around 25% of its genes remain unknown. Thus, it is possible to discover novel resistance genes. One resistance mechanism is the acquisition of genes encoding efflux pumps, among which are the mycobacterial membrane proteins (Mmp), classified as resistance, nodulation and division (RND) efflux pumps. In Mycobacteria, these proteins are involved in the transport of mycolic acid precursors outside the cell wall. In methicillin-resistant S. aureus, an MmpL homolog is involved in oxadiazole resistance. In E. coli, members of this family were characterized in commensal strains as coli K-12, but not in pathogenic ones. In this study we investigated the distribution of mmpL genes (z4861 and yegN) found in E. coli O157:H7 and their potential function by designing knockout mutants and studying the impact of their deletion. Both bioinformatics analysis and PCR screening of clinical isolates demonstrated that yegN is more abundant than z4861. All mutants, when compared to the WT strain, did not show any change in their growth pattern under either aerobic or anaerobic condition. The three mutant strains showed significant increased susceptibility to fluoroquinolones than the WT strain, and this finding was further corroborated by transcriptional analysis that showed a significant increase in z4861 and yegN RNA levels when subjected to ciprofloxacin |
| 530 ## - ADDITIONAL PHYSICAL FORM AVAILABLE NOTE | |
| Additional physical form available note | Issued also as CD |
| 653 #4 - INDEX TERM--UNCONTROLLED | |
| Uncontrolled term | E. coli |
| 653 #4 - INDEX TERM--UNCONTROLLED | |
| Uncontrolled term | Efflux pump |
| 653 #4 - INDEX TERM--UNCONTROLLED | |
| Uncontrolled term | Resistance |
| 700 0# - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Mohamed Abdelaziz Toema , |
| Relator term | |
| 700 0# - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Ramy Karam Aziz , |
| Relator term | |
| 700 0# - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Reham Samir Mohamed , |
| Relator term | |
| 856 ## - ELECTRONIC LOCATION AND ACCESS | |
| Uniform Resource Identifier | <a href="http://172.23.153.220/th.pdf">http://172.23.153.220/th.pdf</a> |
| 905 ## - LOCAL DATA ELEMENT E, LDE (RLIN) | |
| Cataloger | Nazla |
| Reviser | Revisor |
| 905 ## - LOCAL DATA ELEMENT E, LDE (RLIN) | |
| Cataloger | Samia |
| Reviser | Cataloger |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) | |
| Source of classification or shelving scheme | Dewey Decimal Classification |
| Koha item type | Thesis |
| Source of classification or shelving scheme | Not for loan | Home library | Current library | Date acquired | Full call number | Barcode | Date last seen | Koha item type | Copy number |
|---|---|---|---|---|---|---|---|---|---|
| Dewey Decimal Classification | المكتبة المركزبة الجديدة - جامعة القاهرة | قاعة الرسائل الجامعية - الدور الاول | 11.02.2024 | Cai01.08.06.M.Sc.2018.Sa.I | 01010110075790000 | 22.09.2023 | Thesis | ||
| Dewey Decimal Classification | المكتبة المركزبة الجديدة - جامعة القاهرة | مخـــزن الرســائل الجـــامعية - البدروم | 11.02.2024 | Cai01.08.06.M.Sc.2018.Sa.I | 01020110075790000 | 22.09.2023 | CD - Rom | 75790.CD |