Molecular and bioinformatical studies for protein kinase C alpha from cell line / (Record no. 67460)
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| 000 -LEADER | |
|---|---|
| fixed length control field | 03212cam a2200349 a 4500 |
| 003 - CONTROL NUMBER IDENTIFIER | |
| control field | EG-GiCUC |
| 005 - DATE AND TIME OF LATEST TRANSACTION | |
| control field | 20250223032043.0 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION | |
| fixed length control field | 180913s2017 ua h f m 000 0 eng d |
| 040 ## - CATALOGING SOURCE | |
| Original cataloging agency | EG-GiCUC |
| Language of cataloging | eng |
| Transcribing agency | EG-GiCUC |
| 041 0# - LANGUAGE CODE | |
| Language code of text/sound track or separate title | eng |
| 049 ## - LOCAL HOLDINGS (OCLC) | |
| Holding library | Deposite |
| 097 ## - Thesis Degree | |
| Thesis Level | M.Sc |
| 099 ## - LOCAL FREE-TEXT CALL NUMBER (OCLC) | |
| Classification number | Cai01.07.10.M.Sc.2017.Ay.M |
| 100 0# - MAIN ENTRY--PERSONAL NAME | |
| Personal name | Aya Fahmy AboulFotoh Sayed Ahmed Sheha |
| 245 10 - TITLE STATEMENT | |
| Title | Molecular and bioinformatical studies for protein kinase C alpha from cell line / |
| Statement of responsibility, etc. | Aya Fahmy AboulFotoh Sayed Ahmed Sheha ; Supervised Naglaa Abdalmoneim Abdallah , Abdelhadi Abdallah , Haggag Salah Zein |
| 246 15 - VARYING FORM OF TITLE | |
| Title proper/short title | من خط خلوى Protein Kinase C دراسات جزيئية و معلوماتية عن جين |
| 260 ## - PUBLICATION, DISTRIBUTION, ETC. | |
| Place of publication, distribution, etc. | Cairo : |
| Name of publisher, distributor, etc. | Aya Fahmy AboulFotoh Sayed Ahmed Sheha , |
| Date of publication, distribution, etc. | 2017 |
| 300 ## - PHYSICAL DESCRIPTION | |
| Extent | 105 P. : |
| Other physical details | facsimiles ; |
| Dimensions | 25cm |
| 502 ## - DISSERTATION NOTE | |
| Dissertation note | Thesis (M.Sc.) - Cairo University - Faculty of Agriculture - Department of Genetics |
| 520 ## - SUMMARY, ETC. | |
| Summary, etc. | In the last years, protein kinase C (PKC) has become an attractive target for the treatment of cancer patients, its widely described role in carcinogenesis and tumor promotion. Despite the extensive research conducted on these phorbol ester receptors there is only limited knowledge about the contribution of each individual PKC isozyme in malignant transformation, mainly due to the different roles of each isozyme and their tissue-specificity. This diversity provides the unique opportunity to develop specific pharmacological agents, but the complex nature of the signaling pathways activated by different PKCs challenges selective drug therapies. Currently, using of Insilico chemistry and molecular modelling for computer-aided drug design has gained significant momentum for the benefit that it is cost effective in research and development of drugs and also it is now standard for finding new drugs worldwide. In this study, we monitored the impact of fever-range hyperthermia on human breast cancer cell line MCF7 considering cell viability and proliferation. MCF7 breast adenocarcinoma cell line was evaluated after exposure to 37{u00B0}C and 40{u00B0}C, cell viability, cell proliferation, and apoptosis were determined. MCF7 showed reduction in the proliferation activity combined with increasing in PKCÜ expression after exposure to fever range hyperthermia. Point mutation was determined using the PKCÜ gene sequence analysis and revealed a missense mutation in the kinase domain converted a conserved hydrophobic amino acid isoleucine into polar threonine that proved its effect on function alternation from suppression to promotion for the proliferation of the MCF7 breast adenocarcinoma cell line. Also, we rationally designed small interfering ribonucleic acid molecules (siRNAs) as it provides a promising therapeutic modality for the treatment of breast cancer |
| 530 ## - ADDITIONAL PHYSICAL FORM AVAILABLE NOTE | |
| Additional physical form available note | Issued also as CD |
| 653 #4 - INDEX TERM--UNCONTROLLED | |
| Uncontrolled term | PKC |
| 653 #4 - INDEX TERM--UNCONTROLLED | |
| Uncontrolled term | Proliferation |
| 653 #4 - INDEX TERM--UNCONTROLLED | |
| Uncontrolled term | siRNA |
| 700 0# - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Abdelhadi Abdallah , |
| Relator term | |
| 700 0# - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Haggag Salah Zein , |
| Relator term | |
| 700 0# - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Naglaa Abdalmoneim Abdallah , |
| Relator term | |
| 856 ## - ELECTRONIC LOCATION AND ACCESS | |
| Uniform Resource Identifier | <a href="http://172.23.153.220/th.pdf">http://172.23.153.220/th.pdf</a> |
| 905 ## - LOCAL DATA ELEMENT E, LDE (RLIN) | |
| Cataloger | Nazla |
| Reviser | Revisor |
| 905 ## - LOCAL DATA ELEMENT E, LDE (RLIN) | |
| Cataloger | Samia |
| Reviser | Cataloger |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) | |
| Source of classification or shelving scheme | Dewey Decimal Classification |
| Koha item type | Thesis |
| Source of classification or shelving scheme | Not for loan | Home library | Current library | Date acquired | Full call number | Barcode | Date last seen | Koha item type | Copy number |
|---|---|---|---|---|---|---|---|---|---|
| Dewey Decimal Classification | المكتبة المركزبة الجديدة - جامعة القاهرة | قاعة الرسائل الجامعية - الدور الاول | 11.02.2024 | Cai01.07.10.M.Sc.2017.Ay.M | 01010110075794000 | 22.09.2023 | Thesis | ||
| Dewey Decimal Classification | المكتبة المركزبة الجديدة - جامعة القاهرة | مخـــزن الرســائل الجـــامعية - البدروم | 11.02.2024 | Cai01.07.10.M.Sc.2017.Ay.M | 01020110075794000 | 22.09.2023 | CD - Rom | 75794.CD |