Study on the Prevalence of Multidrug- resistant and In vitro Biofilm Formation among Uropathogenic Escherichia coli from Clinical Isolates / (Record no. 68037)
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| 000 -LEADER | |
|---|---|
| fixed length control field | 03671cam a2200349 a 4500 |
| 003 - CONTROL NUMBER IDENTIFIER | |
| control field | EG-GiCUC |
| 005 - DATE AND TIME OF LATEST TRANSACTION | |
| control field | 20250223032102.0 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION | |
| fixed length control field | 181017s2018 ua dh f m 000 0 eng d |
| 040 ## - CATALOGING SOURCE | |
| Original cataloging agency | EG-GiCUC |
| Language of cataloging | eng |
| Transcribing agency | EG-GiCUC |
| 041 0# - LANGUAGE CODE | |
| Language code of text/sound track or separate title | eng |
| 049 ## - LOCAL HOLDINGS (OCLC) | |
| Holding library | Deposite |
| 097 ## - Thesis Degree | |
| Thesis Level | M.Sc |
| 099 ## - LOCAL FREE-TEXT CALL NUMBER (OCLC) | |
| Classification number | Cai01.08.06.M.Sc.2018.We.S |
| 100 0# - MAIN ENTRY--PERSONAL NAME | |
| Personal name | Wesam Elsayed Gawad |
| 245 10 - TITLE STATEMENT | |
| Title | Study on the Prevalence of Multidrug- resistant and In vitro Biofilm Formation among Uropathogenic Escherichia coli from Clinical Isolates / |
| Statement of responsibility, etc. | Wesam Elsayed Gawad ; Supervised Abdelgawad Mohamed Hashem , Wael Mostafa Tawakkol , Omneya Mohamed Helmy |
| 246 15 - VARYING FORM OF TITLE | |
| Title proper/short title | دراسة عن مدى انتشار مقاومة البكتيريا لعديد من المضادات الحيوية و تكوين اغشية بيلوجية في المعمل بين الاشرشيا كولاى المعزولة من البول |
| 260 ## - PUBLICATION, DISTRIBUTION, ETC. | |
| Place of publication, distribution, etc. | Cairo : |
| Name of publisher, distributor, etc. | Wesam Elsayed Gawad , |
| Date of publication, distribution, etc. | 2018 |
| 300 ## - PHYSICAL DESCRIPTION | |
| Extent | 125 P. : |
| Other physical details | charts , facsimiles ; |
| Dimensions | 25cm |
| 502 ## - DISSERTATION NOTE | |
| Dissertation note | Thesis (M.Sc.) - Cairo University - Faculty of Pharmacy - Department of Microbiology and Immunology |
| 520 ## - SUMMARY, ETC. | |
| Summary, etc. | Two hundred and twenty eight UTIs clinical isolates were collected from two Egyptian governorates and identified by conventional culture methods and by the PCR amplification of uspA gene, and classified into the corresponding phylogenetic groups by multiplex PCR. Antimicrobial susceptibility testing was performed using the Kirby-Bauer method. PCR detection of the main genes of the AcrAB-TolC efflux pump was carried out. The contribution of efflux pump-mediated resistance to MDR phenotype was detected by the efflux pump inhibitor microplate-based assay. The ability for in-vitro biofilm formation in UPEC isolates was also tested. The minimum inhibitory concentration (MIC) of tested antibiotics was detected in the presence and absence of sub-inhibitory concentration of EDTA (2 mM) by the two-fold broth microdilution method. The ability for in-vitro biofilm formation was detected in the absence and the presence of 2 concentrations of EDTA (10 mM and 20 mM). The effect of polyvinylchloride Gelatin-EDTA coat on biofilm formation by strong and moderate biofilm producers was also tested. One hundred and seventy five isolates were presumptively identified as E.coli by conventional culture methods, and confirmed by the PCR amplification of uspA. The phylogenetic analysis of the UPEC isolates revealed that most of the isolates belonged to phylogenetic groups B2 (64.57%) and D (18.85%). MDR phenotype was detected in 90.8 % of UPEC isolates. Efflux pump mediated resistance was detected in all MDR isolates. The acrA, acrB and tolC genes were concomitantly detected in 74.84% of MDR isolates. The ability for in vitro biofilm production was recorded in 76.5% of UPEC isolates. The addition of 2 mM EDTA along with antibiotics resulted in a decrease in the antimicrobials MIC values. The highest inhibitory effect of EDTA was observed with meropenem, rendering (81%) of resistant UPEC isolates to completely sensitive ones. EDTA with a concentration of 10mM, 20 mM and Gelatin-EDTA coat inhibited biofilm formation in strong and moderate biofilm producers by 45.8 %, 78.8 % and 81.1%, respectivelybiofilm are needed |
| 530 ## - ADDITIONAL PHYSICAL FORM AVAILABLE NOTE | |
| Additional physical form available note | Issued also as CD |
| 653 #4 - INDEX TERM--UNCONTROLLED | |
| Uncontrolled term | Multidrug- resistant |
| 653 #4 - INDEX TERM--UNCONTROLLED | |
| Uncontrolled term | PCR |
| 653 #4 - INDEX TERM--UNCONTROLLED | |
| Uncontrolled term | Uropathogenic Escherichia coli |
| 700 0# - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Abdelgawad Mohamed Hashem , |
| Relator term | |
| 700 0# - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Omneya Mohamed Helmy , |
| Relator term | |
| 700 0# - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Wael Mostafa Tawakkol , |
| Relator term | |
| 856 ## - ELECTRONIC LOCATION AND ACCESS | |
| Uniform Resource Identifier | <a href="http://172.23.153.220/th.pdf">http://172.23.153.220/th.pdf</a> |
| 905 ## - LOCAL DATA ELEMENT E, LDE (RLIN) | |
| Cataloger | Enas |
| Reviser | Cataloger |
| 905 ## - LOCAL DATA ELEMENT E, LDE (RLIN) | |
| Cataloger | Nazla |
| Reviser | Revisor |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) | |
| Source of classification or shelving scheme | Dewey Decimal Classification |
| Koha item type | Thesis |
| Source of classification or shelving scheme | Not for loan | Home library | Current library | Date acquired | Full call number | Barcode | Date last seen | Koha item type | Copy number |
|---|---|---|---|---|---|---|---|---|---|
| Dewey Decimal Classification | المكتبة المركزبة الجديدة - جامعة القاهرة | قاعة الرسائل الجامعية - الدور الاول | 11.02.2024 | Cai01.08.06.M.Sc.2018.We.S | 01010110076104000 | 22.09.2023 | Thesis | ||
| Dewey Decimal Classification | المكتبة المركزبة الجديدة - جامعة القاهرة | مخـــزن الرســائل الجـــامعية - البدروم | 11.02.2024 | Cai01.08.06.M.Sc.2018.We.S | 01020110076104000 | 22.09.2023 | CD - Rom | 76104.CD |