Evaluation of the effect of mushroom extracts on gene expression in cell-line and microbial growth / (Record no. 71862)
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| 000 -LEADER | |
|---|---|
| fixed length control field | 02776cam a2200349 a 4500 |
| 003 - CONTROL NUMBER IDENTIFIER | |
| control field | EG-GiCUC |
| 005 - DATE AND TIME OF LATEST TRANSACTION | |
| control field | 20250223032257.0 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION | |
| fixed length control field | 190508s2018 ua do f m 000 0 eng d |
| 040 ## - CATALOGING SOURCE | |
| Original cataloging agency | EG-GiCUC |
| Language of cataloging | eng |
| Transcribing agency | EG-GiCUC |
| 041 0# - LANGUAGE CODE | |
| Language code of text/sound track or separate title | eng |
| 049 ## - LOCAL HOLDINGS (OCLC) | |
| Holding library | Deposite |
| 097 ## - Thesis Degree | |
| Thesis Level | M.Sc |
| 099 ## - LOCAL FREE-TEXT CALL NUMBER (OCLC) | |
| Classification number | Cai01.07.10.M.Sc.2018.Am.E |
| 100 0# - MAIN ENTRY--PERSONAL NAME | |
| Personal name | Amal Mohammed Zakaria Mahmoud |
| 245 10 - TITLE STATEMENT | |
| Title | Evaluation of the effect of mushroom extracts on gene expression in cell-line and microbial growth / |
| Statement of responsibility, etc. | Amal Mohammed Zakaria Mahmoud ; Supervised Mona Hashem Hussein , Olfat Sayed Barakat , Salah Eldein Ahmed Mohammeden |
| 246 15 - VARYING FORM OF TITLE | |
| Title proper/short title | تقييم تأثير مستخلصات عيش الغراب علي التعبير الجيني في الخط الخلوي و النمو الميكروبي |
| 260 ## - PUBLICATION, DISTRIBUTION, ETC. | |
| Place of publication, distribution, etc. | Cairo : |
| Name of publisher, distributor, etc. | Amal Mohammed Zakaria Mahmoud , |
| Date of publication, distribution, etc. | 2018 |
| 300 ## - PHYSICAL DESCRIPTION | |
| Extent | 90 P. : |
| Other physical details | charts , photographs ; |
| Dimensions | 25cm |
| 502 ## - DISSERTATION NOTE | |
| Dissertation note | Thesis (M.Sc.) - Cairo University - Faculty of Agriculture - Department of Genetics |
| 520 ## - SUMMARY, ETC. | |
| Summary, etc. | Agaricus bisporus mushroom is nowadays one of the most popular mushrooms around the world, not just because of its delicious taste, but also because of its bioactive compounds and their pharmaceutical values. The current work aims to induce the exopolysaccharides (EPS) production from Agaricus bisporus using different substrate additives, and study its effect as anticancer, antimicrobial and antioxidants substance. Different additives (tryptone, peptone, yeast extract, MgSO4, glucose, lactose, and sucrose) were tested individually using submerged culture for production of mycelia and exopolysaccharides. While tryptone produced the highest amount of mycelia biomass among others, sucrose produced the highest quantity of exopolysaccharides, which is then replaced with molasses by-product as a cheap available natural source of sucrose. Polysaccharide was then applied on MCF-7 cell line, showed no cytotoxic effect, while showed 30% time-independent antiproliferation activity. Also, q-PCR was used for evaluation of different apoptotic gene expression; TNFÜ, P53, Caspase9, BCL2, and Bax, in which TNFÜ showed the highest expression level (2.18 folds) after treatment. Assessment of apoptosis and cell cycle arresting using Flowcytometry, revealed that polysaccharides arrest G1/G2 phase, resulting in 5.5%apoptosis in treated cells |
| 530 ## - ADDITIONAL PHYSICAL FORM AVAILABLE NOTE | |
| Additional physical form available note | Issued also as CD |
| 653 #4 - INDEX TERM--UNCONTROLLED | |
| Uncontrolled term | Agaricus bisporus |
| 653 #4 - INDEX TERM--UNCONTROLLED | |
| Uncontrolled term | Anticancer |
| 653 #4 - INDEX TERM--UNCONTROLLED | |
| Uncontrolled term | Polysaccharides |
| 700 0# - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Mona Hashem Hussein , |
| Relator term | |
| 700 0# - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Olfat Sayed Barakat , |
| Relator term | |
| 700 0# - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Salah Eldein Ahmed Mohammeden , |
| Relator term | |
| 856 ## - ELECTRONIC LOCATION AND ACCESS | |
| Uniform Resource Identifier | <a href="http://172.23.153.220/th.pdf">http://172.23.153.220/th.pdf</a> |
| 905 ## - LOCAL DATA ELEMENT E, LDE (RLIN) | |
| Cataloger | Nazla |
| Reviser | Revisor |
| 905 ## - LOCAL DATA ELEMENT E, LDE (RLIN) | |
| Cataloger | Rania |
| Reviser | Cataloger |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) | |
| Source of classification or shelving scheme | Dewey Decimal Classification |
| Koha item type | Thesis |
| Source of classification or shelving scheme | Not for loan | Home library | Current library | Date acquired | Full call number | Barcode | Date last seen | Koha item type | Copy number |
|---|---|---|---|---|---|---|---|---|---|
| Dewey Decimal Classification | المكتبة المركزبة الجديدة - جامعة القاهرة | قاعة الرسائل الجامعية - الدور الاول | 11.02.2024 | Cai01.07.10.M.Sc.2018.Am.E | 01010110078086000 | 22.09.2023 | Thesis | ||
| Dewey Decimal Classification | المكتبة المركزبة الجديدة - جامعة القاهرة | مخـــزن الرســائل الجـــامعية - البدروم | 11.02.2024 | Cai01.07.10.M.Sc.2018.Am.E | 01020110078086000 | 22.09.2023 | CD - Rom | 78086.CD |