Rapid detection of bacterial food borne pathogens by using molecular techniques / (Record no. 73245)
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| 000 -LEADER | |
|---|---|
| fixed length control field | 02647cam a2200349 a 4500 |
| 003 - CONTROL NUMBER IDENTIFIER | |
| control field | EG-GiCUC |
| 005 - DATE AND TIME OF LATEST TRANSACTION | |
| control field | 20250223032339.0 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION | |
| fixed length control field | 190803s2019 ua dh f m 000 0 eng d |
| 040 ## - CATALOGING SOURCE | |
| Original cataloging agency | EG-GiCUC |
| Language of cataloging | eng |
| Transcribing agency | EG-GiCUC |
| 041 0# - LANGUAGE CODE | |
| Language code of text/sound track or separate title | eng |
| 049 ## - LOCAL HOLDINGS (OCLC) | |
| Holding library | Deposite |
| 097 ## - Thesis Degree | |
| Thesis Level | Ph.D |
| 099 ## - LOCAL FREE-TEXT CALL NUMBER (OCLC) | |
| Classification number | Cai01.10.10.Ph.D.2019.Ze.R |
| 100 0# - MAIN ENTRY--PERSONAL NAME | |
| Personal name | Zeinab Abdelbadiea Elsayed |
| 245 10 - TITLE STATEMENT | |
| Title | Rapid detection of bacterial food borne pathogens by using molecular techniques / |
| Statement of responsibility, etc. | Zeinab Abdelbadiea Elsayed ; Supervised Jakeen Kamal Abdelhaleem Eljakee , Soad Abdelaziz Abdelwanis , Rehab Ali Mohamed Elhelw |
| 246 15 - VARYING FORM OF TITLE | |
| Title proper/short title | الكشف السريع عن مسببات الأمراض البكتريه التي تنقلها الاغذيه باستخدام التقنيات الجزئييه |
| 260 ## - PUBLICATION, DISTRIBUTION, ETC. | |
| Place of publication, distribution, etc. | Cairo : |
| Name of publisher, distributor, etc. | Zeinab Abdelbadiea Elsayed , |
| Date of publication, distribution, etc. | 2019 |
| 300 ## - PHYSICAL DESCRIPTION | |
| Extent | 104 P. : |
| Other physical details | charts , facsimiles ; |
| Dimensions | 25cm |
| 502 ## - DISSERTATION NOTE | |
| Dissertation note | Thesis (Ph.D.) - Cairo University - Faculty of Veterinary Medicine - Department of Microbiology |
| 520 ## - SUMMARY, ETC. | |
| Summary, etc. | Rapid detection of pathogens in food becomes a critical and important demand for human safety, since most foodborne illnesses and deaths are caused by pathogenic bacteria. So application of rapid, sensitive method to detect foodborne pathogen is essential in controlling food safety. In this study, a two multiplex polymerase chain reaction (mPCR) technique for the simultaneous detection of some foodborne pathogens (Salmonella ,S. aureus , Bacillus cereus , Listeria monocytogenes, E. coli and Campylobacter spp.) was done in culture broth and artificial food matrix. Pathogen-specific DNA sequences in the invA, clfA, groEL, 16S rRNA, phoA and 23S rRNA genes were used as targets to design primers for the identification of Salmonella, S. aureus, Bacillus cereus, Listeria monocytogenes, E. coli and Campylobacter spp. respectively. The detection of sensitivity in this assay was 10 CFU/ml of each pathogen in a culture broth and artificially inoculated samples after enrichment for 24 h. The mPCR assay proposed here can gain results within 24 h and correspond to the results obtained by the classical cultivation based on ISO methods, which will be valuable for food safety investigations |
| 530 ## - ADDITIONAL PHYSICAL FORM AVAILABLE NOTE | |
| Additional physical form available note | Issued also as CD |
| 653 #4 - INDEX TERM--UNCONTROLLED | |
| Uncontrolled term | mPCR- foodborne pathogens |
| 653 #4 - INDEX TERM--UNCONTROLLED | |
| Uncontrolled term | S. aureus |
| 653 #4 - INDEX TERM--UNCONTROLLED | |
| Uncontrolled term | Salmonella |
| 700 0# - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Jakeen Kamal Abdelhaleem Eljakee , |
| Relator term | |
| 700 0# - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Rehab Ali Mohamed Elhelw , |
| Relator term | |
| 700 0# - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Soad Abdelaziz Abdelwanis , |
| Relator term | |
| 856 ## - ELECTRONIC LOCATION AND ACCESS | |
| Uniform Resource Identifier | <a href="http://172.23.153.220/th.pdf">http://172.23.153.220/th.pdf</a> |
| 905 ## - LOCAL DATA ELEMENT E, LDE (RLIN) | |
| Cataloger | Nazla |
| Reviser | Revisor |
| 905 ## - LOCAL DATA ELEMENT E, LDE (RLIN) | |
| Cataloger | Shimaa |
| Reviser | Cataloger |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) | |
| Source of classification or shelving scheme | Dewey Decimal Classification |
| Koha item type | Thesis |
| Source of classification or shelving scheme | Not for loan | Home library | Current library | Date acquired | Full call number | Barcode | Date last seen | Koha item type | Copy number |
|---|---|---|---|---|---|---|---|---|---|
| Dewey Decimal Classification | المكتبة المركزبة الجديدة - جامعة القاهرة | قاعة الرسائل الجامعية - الدور الاول | 11.02.2024 | Cai01.10.10.Ph.D.2019.Ze.R | 01010110078867000 | 22.09.2023 | Thesis | ||
| Dewey Decimal Classification | المكتبة المركزبة الجديدة - جامعة القاهرة | مخـــزن الرســائل الجـــامعية - البدروم | 11.02.2024 | Cai01.10.10.Ph.D.2019.Ze.R | 01020110078867000 | 22.09.2023 | CD - Rom | 78867.CD |