Investigating the role of some folate biosynthesis pathway enzymes in survival of escherichia coli / (Record no. 75023)
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| 000 -LEADER | |
|---|---|
| fixed length control field | 03333cam a2200337 a 4500 |
| 003 - CONTROL NUMBER IDENTIFIER | |
| control field | EG-GiCUC |
| 005 - DATE AND TIME OF LATEST TRANSACTION | |
| control field | 20250223032432.0 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION | |
| fixed length control field | 191106s2019 ua d f m 000 0 eng d |
| 040 ## - CATALOGING SOURCE | |
| Original cataloging agency | EG-GiCUC |
| Language of cataloging | eng |
| Transcribing agency | EG-GiCUC |
| 041 0# - LANGUAGE CODE | |
| Language code of text/sound track or separate title | eng |
| 049 ## - LOCAL HOLDINGS (OCLC) | |
| Holding library | Deposite |
| 097 ## - Thesis Degree | |
| Thesis Level | M.Sc |
| 099 ## - LOCAL FREE-TEXT CALL NUMBER (OCLC) | |
| Classification number | Cai01.08.06.M.Sc.2019.Di.I |
| 100 0# - MAIN ENTRY--PERSONAL NAME | |
| Personal name | Dina Samir Rasmi |
| 245 10 - TITLE STATEMENT | |
| Title | Investigating the role of some folate biosynthesis pathway enzymes in survival of escherichia coli / |
| Statement of responsibility, etc. | Dina Samir Rasmi ; Supervised Ramy Karam Aziz , Yomna Abdelawad Hashem |
| 246 15 - VARYING FORM OF TITLE | |
| Title proper/short title | في النمو بكتيريا الإشريكيا القولونية Folate دراسة دور بعض إنزيمات المسار الحيوى للفولات |
| 260 ## - PUBLICATION, DISTRIBUTION, ETC. | |
| Place of publication, distribution, etc. | Cairo : |
| Name of publisher, distributor, etc. | Dina Samir Rasmi , |
| Date of publication, distribution, etc. | 2019 |
| 300 ## - PHYSICAL DESCRIPTION | |
| Extent | 109 P. : |
| Other physical details | charts ; |
| Dimensions | 25cm |
| 502 ## - DISSERTATION NOTE | |
| Dissertation note | Thesis (M.Sc.) - Cairo University - Faculty of Pharmacy - Department of Microbiology and Immunology |
| 520 ## - SUMMARY, ETC. | |
| Summary, etc. | Folate biosynthesis in bacteria has been extensively studied, but the essentiality of one of the steps in the pathway, dihydroneopterin triphosphate (DHNTP) hydrolysis to dihydroneopterin monophosphate (DHNMP), is yet to be demonstrated. A candidate enzyme catalyzing this step, dihydroneopterin hydrolase, was confirmed to affect folate biosynthesis; however, the deletion of its encoding gene, ntpA, does not result in bacterial lethality. Computational modeling suggests the possibility that other phosphatases such as mutT or the broad-spectrum phoA may be compensating for the loss of ntpA. To investigate the role of these two genes, we constructed, in Escherichia coli K-12 MG1655, single, double and triple mutants lacking mutT or phoA, in addition to ntpA. Growth experiments were conducted on the wild type and mutant strains. The triple mutant, xmutTxntpAxphoA, failed to grow on M9 minimal media, which suggests that mutT, ntpA and phoA together are essential for the bacterial growth in minimal media, while the growth of the double mutant,xmutTxntpA, was significantly slower. Quantitative reverse-transcription PCR (qRT-PCR) indicated that the expression of phoA and mutT, but not that of ntpA, significantly increased in response to sub-minimal inhibitory concentrations of sulfonamides, confirming the involvement of the genes in the folate pathway. More transcriptional analysis was performed on the single and double mutants to study the expression of each gene in absence of its putative complementary one(s), but there was no significant upregulation at the gene expression level. For a better understanding of the function of the three genes, transcriptomic data from the Gene Expression Omnibus (GEO) resource were analyzed to determine how different conditions might affect the expression of the ntpA, mutT and phoA in E. coli K-12. Subsequently, qRT-PCR was used to confirm these computational predictions. Culture density increase was demonstrated to decrease the expression of ntpA, mutT while increasing phoA expression |
| 530 ## - ADDITIONAL PHYSICAL FORM AVAILABLE NOTE | |
| Additional physical form available note | Issued also as CD |
| 653 #4 - INDEX TERM--UNCONTROLLED | |
| Uncontrolled term | DHNTPase |
| 653 #4 - INDEX TERM--UNCONTROLLED | |
| Uncontrolled term | Folate pathway |
| 653 #4 - INDEX TERM--UNCONTROLLED | |
| Uncontrolled term | ntpA |
| 700 0# - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Ramy Karam Aziz , |
| Relator term | |
| 700 0# - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Yomna Abdelawad Hashem , |
| Relator term | |
| 856 ## - ELECTRONIC LOCATION AND ACCESS | |
| Uniform Resource Identifier | <a href="http://172.23.153.220/th.pdf">http://172.23.153.220/th.pdf</a> |
| 905 ## - LOCAL DATA ELEMENT E, LDE (RLIN) | |
| Cataloger | Nazla |
| Reviser | Revisor |
| 905 ## - LOCAL DATA ELEMENT E, LDE (RLIN) | |
| Cataloger | Shimaa |
| Reviser | Cataloger |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) | |
| Source of classification or shelving scheme | Dewey Decimal Classification |
| Koha item type | Thesis |
| Source of classification or shelving scheme | Not for loan | Home library | Current library | Date acquired | Full call number | Barcode | Date last seen | Koha item type | Copy number |
|---|---|---|---|---|---|---|---|---|---|
| Dewey Decimal Classification | المكتبة المركزبة الجديدة - جامعة القاهرة | قاعة الرسائل الجامعية - الدور الاول | 11.02.2024 | Cai01.08.06.M.Sc.2019.Di.I | 01010110079824000 | 22.09.2023 | Thesis | ||
| Dewey Decimal Classification | المكتبة المركزبة الجديدة - جامعة القاهرة | مخـــزن الرســائل الجـــامعية - البدروم | 11.02.2024 | Cai01.08.06.M.Sc.2019.Di.I | 01020110079824000 | 22.09.2023 | CD - Rom | 79824.CD |