Spoligotyping with pncA sequencing strategy conferring the transmission of multidrug{u2011}resistant tuberculosis in Egypt / (Record no. 77005)
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| 000 -LEADER | |
|---|---|
| fixed length control field | 02944cam a2200349 a 4500 |
| 003 - CONTROL NUMBER IDENTIFIER | |
| control field | EG-GiCUC |
| 005 - DATE AND TIME OF LATEST TRANSACTION | |
| control field | 20250223032534.0 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION | |
| fixed length control field | 200321s2019 ua dh f m 000 0 eng d |
| 040 ## - CATALOGING SOURCE | |
| Original cataloging agency | EG-GiCUC |
| Language of cataloging | eng |
| Transcribing agency | EG-GiCUC |
| 041 0# - LANGUAGE CODE | |
| Language code of text/sound track or separate title | eng |
| 049 ## - LOCAL HOLDINGS (OCLC) | |
| Holding library | Deposite |
| 097 ## - Thesis Degree | |
| Thesis Level | Ph.D |
| 099 ## - LOCAL FREE-TEXT CALL NUMBER (OCLC) | |
| Classification number | Cai01.10.10.Ph.D.2019.Su.S |
| 100 0# - MAIN ENTRY--PERSONAL NAME | |
| Personal name | Susan Abdelnafea Ismail |
| 245 10 - TITLE STATEMENT | |
| Title | Spoligotyping with pncA sequencing strategy conferring the transmission of multidrug{u2011}resistant tuberculosis in Egypt / |
| Statement of responsibility, etc. | Susan Abdelnafea Ismail ; Supervised Khaled Farouk Mohammed Elamry , Salah Eldin Abdelkarim Selim , Mohamed Galal Aggour |
| 246 15 - VARYING FORM OF TITLE | |
| Title proper/short title | استراتيجيه تصنيف سبوليجو مع تتابع بى ان سى ايه للتعرف على انتقال السل متعدد المقاومه للعقاقير فى مصر |
| 260 ## - PUBLICATION, DISTRIBUTION, ETC. | |
| Place of publication, distribution, etc. | Cairo : |
| Name of publisher, distributor, etc. | Susan Abdelnafea Ismail , |
| Date of publication, distribution, etc. | 2019 |
| 300 ## - PHYSICAL DESCRIPTION | |
| Extent | 92 P. : |
| Other physical details | charts , facsimiles ; |
| Dimensions | 25cm |
| 502 ## - DISSERTATION NOTE | |
| Dissertation note | Thesis (Ph.D.) - Cairo University - Faculty of Veterinary Medicine - Department of Microbiology |
| 520 ## - SUMMARY, ETC. | |
| Summary, etc. | This study explored the genetic diversity of Mycobacterium tuberculosis isolates in Egypt by spoligotyping in combination with pncA gene sequencing, spoNC. Firstly, 50 isolates out of 400 isolates positive for Mycobacterium tuberculosis were referred to Central Labs Ministry of health were subjected to the analyses, 21 isolates were found to be multidrug resistant (MDR) and 29 isoletes were sensitive for isonizide(INH) and rifampicine(RIF) after testing by phenotypic drug susceptibility testing (DST) and MGIT.Spoligotyping yielded 45 patterns belonging to 7 families that were previously reported in neighboring countries as Iraq, Syria, Iran and Turky. while 4 isolates were orphans. Application of spoNC on obtained Spoligotype patterns enhance to reduce the clustering rate. Bejing family the predominant (34%)were subdivided by pncA sequence into 3 sensitive DST pncA wild type , 3 MDR-DST isolates showing cys14Arg mutation in pncA, 2 sensitive DST isolates with pncA Gly97Asp mutation, and 3 sensitive DST pncAVal128Gly mutation.The next most common CASI_DELHI family (16%) were subdivided by pncA sequencing into CASI_DELHI (st 381, MDR) including 2 pncA silent mutation ser65ser (tcc>tct) and CASI_DELHI (st26, sensitive) which included 6 pncA (WT) results, LAM6 family(6%) all had PncA Gly97Asp mutation, We concluded that SpoNC providesgood snap shot for MDR surveillance and its country origin, and performing early identification of outbreaks in Egyp |
| 530 ## - ADDITIONAL PHYSICAL FORM AVAILABLE NOTE | |
| Additional physical form available note | Issued also as CD |
| 653 #4 - INDEX TERM--UNCONTROLLED | |
| Uncontrolled term | Egypt |
| 653 #4 - INDEX TERM--UNCONTROLLED | |
| Uncontrolled term | Mycobcterium |
| 653 #4 - INDEX TERM--UNCONTROLLED | |
| Uncontrolled term | SpoNC |
| 700 0# - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Khaled Farouk Mohammed Elamry , |
| Relator term | |
| 700 0# - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Mohamed Galal Aggour , |
| Relator term | |
| 700 0# - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Salah Eldin Abdelkarim Selim , |
| Relator term | |
| 856 ## - ELECTRONIC LOCATION AND ACCESS | |
| Uniform Resource Identifier | <a href="http://172.23.153.220/th.pdf">http://172.23.153.220/th.pdf</a> |
| 905 ## - LOCAL DATA ELEMENT E, LDE (RLIN) | |
| Cataloger | Nazla |
| Reviser | Revisor |
| 905 ## - LOCAL DATA ELEMENT E, LDE (RLIN) | |
| Cataloger | Shimaa |
| Reviser | Cataloger |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) | |
| Source of classification or shelving scheme | Dewey Decimal Classification |
| Koha item type | Thesis |
| Source of classification or shelving scheme | Not for loan | Home library | Current library | Date acquired | Full call number | Barcode | Date last seen | Koha item type | Copy number |
|---|---|---|---|---|---|---|---|---|---|
| Dewey Decimal Classification | المكتبة المركزبة الجديدة - جامعة القاهرة | قاعة الرسائل الجامعية - الدور الاول | 11.02.2024 | Cai01.10.10.Ph.D.2019.Su.S | 01010110081073000 | 22.09.2023 | Thesis | ||
| Dewey Decimal Classification | المكتبة المركزبة الجديدة - جامعة القاهرة | مخـــزن الرســائل الجـــامعية - البدروم | 11.02.2024 | Cai01.10.10.Ph.D.2019.Su.S | 01020110081073000 | 22.09.2023 | CD - Rom | 81073.CD |