Ultra- structure, transcriptional profile and embryonic development of Egyptian buffalo oocytes matured in vitro under oxidative stress / (رقم التسجيلة. 77859)
[ عرض عادي ]
| 000 -LEADER | |
|---|---|
| fixed length control field | 03063cam a2200337 a 4500 |
| 003 - CONTROL NUMBER IDENTIFIER | |
| control field | EG-GiCUC |
| 005 - DATE AND TIME OF LATEST TRANSACTION | |
| control field | 20250223032601.0 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION | |
| fixed length control field | 200922s2020 ua dh f m 000 0 eng d |
| 040 ## - CATALOGING SOURCE | |
| Original cataloging agency | EG-GiCUC |
| Language of cataloging | eng |
| Transcribing agency | EG-GiCUC |
| 041 0# - LANGUAGE CODE | |
| Language code of text/sound track or separate title | eng |
| 049 ## - LOCAL HOLDINGS (OCLC) | |
| Holding library | Deposite |
| 097 ## - Thesis Degree | |
| Thesis Level | Ph.D |
| 099 ## - LOCAL FREE-TEXT CALL NUMBER (OCLC) | |
| Classification number | Cai01.07.03.Ph.D.2020.Da.u |
| 100 0# - MAIN ENTRY--PERSONAL NAME | |
| Personal name | Dalia Abdelrahman Ahmed |
| 245 10 - TITLE STATEMENT | |
| Title | Ultra- structure, transcriptional profile and embryonic development of Egyptian buffalo oocytes matured in vitro under oxidative stress / |
| Statement of responsibility, etc. | Dalia Abdelrahman Ahmed ; Supervised Nasser Ghanem Osman , Sherif Mohamed Dessouki |
| 246 15 - VARYING FORM OF TITLE | |
| Title proper/short title | التركيب الخلوى الدقيق: التعبير الجينى وتطور الأجنة لبويضات الجاموس المصرى الناضجة معمليًا تحت ظروف الإجهاد التأكسدى |
| 260 ## - PUBLICATION, DISTRIBUTION, ETC. | |
| Place of publication, distribution, etc. | Cairo : |
| Name of publisher, distributor, etc. | Dalia Abdelrahman Ahmed , |
| Date of publication, distribution, etc. | 2020 |
| 300 ## - PHYSICAL DESCRIPTION | |
| Extent | 137 P. : |
| Other physical details | charts , facsmilies ; |
| Dimensions | 25cm |
| 502 ## - DISSERTATION NOTE | |
| Dissertation note | Thesis (Ph.D.) - Cairo University - Faculty of Agriculture - Department of Animal Production |
| 520 ## - SUMMARY, ETC. | |
| Summary, etc. | This study was performed to investigate cellular and molecular changes of buffalo cumulus-oocyte complexes (COCs) cultured under oxidative stress, and to assess developmental competence after maturation through, in vitro fertilization and early embryonic development up to the blastocyst stage. Morphologically good quality COCs (n=2672) were screened with brilliant cresy- blue (BCB) staining and then graded into three groups (BCB+, BCB - and control). Oocytes from the different groups were evaluated under low (5%) and high oxygen level (20 %), The progress of the nuclear maturation stage and the rate of cumulus expansion were assessed after 24 hrs of in vitro maturation. Ultra-structure of oocytes have been monitored using transmission electron microscope.The fluorescent intensity of lipid, mitochondria, and reactive oxygen species (ROS) were measured using different molecular probes. Transcript abundance of genes regulating different molecular pathways during oocyte maturation was profiled using Real-time PCR. The cumulus expansion of all oocyte groups under low oxygen tension (5%) was higher (P < 0.05) than those cultured under high oxygen tension (20%). Under high oxygen tension, low-competent oocytes (BCB -) showed the lowest maturation rate (P < 0.05). This was associated with an increase in ROS, which was against the BCB+ oocyte pattern. Ultra-structure examination indicated that under low oxygen tension the competent BCB+ oocytes had a higher rate of migration of cortical granules than BCB -. In parallel, the MAPK14 and CPT2 gene expression profile (P < 0.05) was increased under low compared to high oxygen tension |
| 530 ## - ADDITIONAL PHYSICAL FORM AVAILABLE NOTE | |
| Additional physical form available note | Issued also as CD |
| 653 #4 - INDEX TERM--UNCONTROLLED | |
| Uncontrolled term | BCB |
| 653 #4 - INDEX TERM--UNCONTROLLED | |
| Uncontrolled term | Oocytes |
| 653 #4 - INDEX TERM--UNCONTROLLED | |
| Uncontrolled term | Oxidative stress |
| 700 0# - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Nasser Ghanem Osman , |
| Relator term | |
| 700 0# - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Sherif Mohamed Dessouki , |
| Relator term | |
| 856 ## - ELECTRONIC LOCATION AND ACCESS | |
| Uniform Resource Identifier | <a href="http://172.23.153.220/th.pdf">http://172.23.153.220/th.pdf</a> |
| 905 ## - LOCAL DATA ELEMENT E, LDE (RLIN) | |
| Cataloger | Amira |
| Reviser | Cataloger |
| 905 ## - LOCAL DATA ELEMENT E, LDE (RLIN) | |
| Cataloger | Nazla |
| Reviser | Revisor |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) | |
| Source of classification or shelving scheme | Dewey Decimal Classification |
| Koha item type | Thesis |
| Source of classification or shelving scheme | Not for loan | Home library | Current library | Date acquired | Full call number | Barcode | Date last seen | Koha item type | Copy number |
|---|---|---|---|---|---|---|---|---|---|
| Dewey Decimal Classification | المكتبة المركزبة الجديدة - جامعة القاهرة | قاعة الرسائل الجامعية - الدور الاول | 11.02.2024 | Cai01.07.03.Ph.D.2020.Da.u | 01010110081522000 | 22.09.2023 | Thesis | ||
| Dewey Decimal Classification | المكتبة المركزبة الجديدة - جامعة القاهرة | مخـــزن الرســائل الجـــامعية - البدروم | 11.02.2024 | Cai01.07.03.Ph.D.2020.Da.u | 01020110081522000 | 22.09.2023 | CD - Rom | 81522.CD |