Optimization of the production and purification of lysostaphin and its application to fight staphylococcal biofilms / (Record no. 77964)
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| 000 -LEADER | |
|---|---|
| fixed length control field | 03062cam a2200337 a 4500 |
| 003 - CONTROL NUMBER IDENTIFIER | |
| control field | EG-GiCUC |
| 005 - DATE AND TIME OF LATEST TRANSACTION | |
| control field | 20250223032605.0 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION | |
| fixed length control field | 200929s2020 ua dh f m 000 0 eng d |
| 040 ## - CATALOGING SOURCE | |
| Original cataloging agency | EG-GiCUC |
| Language of cataloging | eng |
| Transcribing agency | EG-GiCUC |
| 041 0# - LANGUAGE CODE | |
| Language code of text/sound track or separate title | eng |
| 049 ## - LOCAL HOLDINGS (OCLC) | |
| Holding library | Deposite |
| 097 ## - Thesis Degree | |
| Thesis Level | Ph.D |
| 099 ## - LOCAL FREE-TEXT CALL NUMBER (OCLC) | |
| Classification number | Cai01.08.06.Ph.D.2020.Ha.O |
| 100 0# - MAIN ENTRY--PERSONAL NAME | |
| Personal name | Hanzada Tawfik Mohamed Nour Eldin |
| 245 10 - TITLE STATEMENT | |
| Title | Optimization of the production and purification of lysostaphin and its application to fight staphylococcal biofilms / |
| Statement of responsibility, etc. | Hanzada Tawfik Mohamed Nour Eldin ; Supervised Manal Mohamed Maher , Ahmed Sherif Attia , |
| 246 15 - VARYING FORM OF TITLE | |
| Title proper/short title | تحس{u٠٦أأ}ن إنتاج وتنق{u٠٦أأ}ة الل{u٠٦أأ}زوستاف{u٠٦أأ}ن وتطب{u٠٦أأ}قاته لمكافحة العنقود{u٠٦أأ}ات فى الأغش{u٠٦أأ}ة الح{u٠٦أأ}و{u٠٦أأ}ة |
| 260 ## - PUBLICATION, DISTRIBUTION, ETC. | |
| Place of publication, distribution, etc. | Cairo : |
| Name of publisher, distributor, etc. | Hanzada Tawfik Mohamed Nour Eldin , |
| Date of publication, distribution, etc. | 2020 |
| 300 ## - PHYSICAL DESCRIPTION | |
| Extent | 149 P. : |
| Other physical details | charts , facsmilies ; |
| Dimensions | 25cm |
| 502 ## - DISSERTATION NOTE | |
| Dissertation note | Thesis (Ph.D.) - Cairo University - Faculty of Pharmacy - Department of Microbiology and Immunology |
| 520 ## - SUMMARY, ETC. | |
| Summary, etc. | Lysostaphin (LST) is an extracellular zinc metalloproteinase bacteriocin secreted by Staphylococcus simulans biovar staphylolyticus that destroys staphylococcal cell wall through its endopeptidase activity. S. aureus imposes a threatening public health issue;hence, interest is raised back in LST.However, its usage is limited due to the lack of affordable, highly-active preparations. Herein the main objective was directed towards achieving sustainable and low-cost LST production platform, using both recombinant and native lysostaphin,to be used in combating S.aureus infections. Recombinant DNA technology was used to clone, express, and purify LST as an N-terminal His-tagged protein in Escherichia coli. A full factorial design was developed in which induction was optimized, being reflected in minimal cost and time. The produced rLST was evaluated in combating biofilms using crystal-violet based assays. This work was further expanded by aiming to develop a novel rLST topical formula targeting staphylococcal skin infections and assessing it in a murine skin model of infection. The rLST optimized production process yielded up to 50 mg/L culture, which is higher than previously reported systems.The purified rLST demonstrated superlative activity in inhibiting staphylococcal biofilms formation as well as detachment of previously established ones, compared to vancomycin and linezolid. Finally, phase inversion composition (PIC) method was successful in incorporating LST in a nano-emulgel (LNEG) which was able to extend active LST release up to 8 h and cure skin infections in a murine skin model |
| 530 ## - ADDITIONAL PHYSICAL FORM AVAILABLE NOTE | |
| Additional physical form available note | Issued also as CD |
| 653 #4 - INDEX TERM--UNCONTROLLED | |
| Uncontrolled term | Factorial-design |
| 653 #4 - INDEX TERM--UNCONTROLLED | |
| Uncontrolled term | lysostaphin |
| 653 #4 - INDEX TERM--UNCONTROLLED | |
| Uncontrolled term | Sustainable |
| 700 0# - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Ahmed Sherif Attia , |
| Relator term | |
| 700 0# - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Manal Mohamed Maher , |
| Relator term | |
| 856 ## - ELECTRONIC LOCATION AND ACCESS | |
| Uniform Resource Identifier | <a href="http://172.23.153.220/th.pdf">http://172.23.153.220/th.pdf</a> |
| 905 ## - LOCAL DATA ELEMENT E, LDE (RLIN) | |
| Cataloger | Amira |
| Reviser | Cataloger |
| 905 ## - LOCAL DATA ELEMENT E, LDE (RLIN) | |
| Cataloger | Nazla |
| Reviser | Revisor |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) | |
| Source of classification or shelving scheme | Dewey Decimal Classification |
| Koha item type | Thesis |
| Source of classification or shelving scheme | Not for loan | Home library | Current library | Date acquired | Full call number | Barcode | Date last seen | Koha item type | Copy number |
|---|---|---|---|---|---|---|---|---|---|
| Dewey Decimal Classification | المكتبة المركزبة الجديدة - جامعة القاهرة | قاعة الرسائل الجامعية - الدور الاول | 11.02.2024 | Cai01.08.06.Ph.D.2020.Ha.O | 01010110081571000 | 22.09.2023 | Thesis | ||
| Dewey Decimal Classification | المكتبة المركزبة الجديدة - جامعة القاهرة | مخـــزن الرســائل الجـــامعية - البدروم | 11.02.2024 | Cai01.08.06.Ph.D.2020.Ha.O | 01020110081571000 | 22.09.2023 | CD - Rom | 81571.CD |