Targeting signaling pathways activated in drug resistant breast cancer cell line / (رقم التسجيلة. 78138)
[ عرض عادي ]
| 000 -LEADER | |
|---|---|
| fixed length control field | 03079cam a2200349 a 4500 |
| 003 - CONTROL NUMBER IDENTIFIER | |
| control field | EG-GiCUC |
| 005 - DATE AND TIME OF LATEST TRANSACTION | |
| control field | 20250223032610.0 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION | |
| fixed length control field | 201010s2020 ua dh f m 000 0 eng d |
| 040 ## - CATALOGING SOURCE | |
| Original cataloging agency | EG-GiCUC |
| Language of cataloging | eng |
| Transcribing agency | EG-GiCUC |
| 041 0# - LANGUAGE CODE | |
| Language code of text/sound track or separate title | eng |
| 049 ## - LOCAL HOLDINGS (OCLC) | |
| Holding library | Deposite |
| 097 ## - Thesis Degree | |
| Thesis Level | Ph.D |
| 099 ## - LOCAL FREE-TEXT CALL NUMBER (OCLC) | |
| Classification number | Cai01.19.02.Ph.D.2020.Ya.T |
| 100 0# - MAIN ENTRY--PERSONAL NAME | |
| Personal name | Yasmin Mostafa Attia |
| 245 10 - TITLE STATEMENT | |
| Title | Targeting signaling pathways activated in drug resistant breast cancer cell line / |
| Statement of responsibility, etc. | Yasmin Mostafa Attia ; Supervised Samia A. Shouman , Salama A. Salama , Gabriel B. Lopez |
| 246 15 - VARYING FORM OF TITLE | |
| Title proper/short title | استهداف المسارات النشطة المفعلة فى خلايا سرطان الثدى المقاومة للادوية |
| 260 ## - PUBLICATION, DISTRIBUTION, ETC. | |
| Place of publication, distribution, etc. | Cairo : |
| Name of publisher, distributor, etc. | Yasmin Mostafa Attia , |
| Date of publication, distribution, etc. | 2020 |
| 300 ## - PHYSICAL DESCRIPTION | |
| Extent | 148 P. : |
| Other physical details | charts , facsimile ; |
| Dimensions | 25cm |
| 502 ## - DISSERTATION NOTE | |
| Dissertation note | Thesis (Ph.D.) - Cairo University - National Cancer Institute - Department of Cancer Biology |
| 520 ## - SUMMARY, ETC. | |
| Summary, etc. | Background:Tamoxifen is the gold-standard endocrine therapy for estrogen receptor positive (ER+) breast cancer. Nonetheless, tamoxifen resistance is a challenging clinical problem. Transcriptional activation of ER-Ü can be stimulated by cyclin-dependent kinase 7 (CDK7), which plays an important role in the regulation of cell cycle and the expression of genes involved in tumor progression and resistance to tamoxifen. Aim of the work: To study the potential modulatory role of CDK7 inhibitor in tamoxifen response and to explore its mechanistic pathways in the tamoxifen sensitive, MCF-7 and tamoxifen-resistant LCC2, breast cancer cell lines; in two orthotopic xenograft models, as well as in clinical samples. Methods: The cytotoxic effect of CDK7 inhibition, alone and in combination with tamoxifen was studied in vitro, in both tamoxifen-sensitive and resistant cell lines, as well as in orthotopic breast cancer mouse models. Drug interaction was evaluated using the isobologram equation. Apoptosis was evaluated by flow cytometry and TUNEL assay. The expression of angiogenesis marker (CD31), cell cycle regulatory proteins as well as downstream oncogenic markers were determined by flow cytometry and western blotting. Moreover, analysis of TCGA breast cancer data and the microarray data of patients receiving tamoxifen were investigated to evaluate the role of CDK7 in tamoxifen resistance. Results: The current in vitro study showed that CDK7 inhibitors either by siRNA or pharmacologically by THZ1 decreased the expression of activated estrogen receptor-Ü (p-ER-Ü S118) and downregulated the of essential oncogenes such as MYC, STAT3 and Ý{u2013}catenin expression which plays a crucial role in tamoxifen resistance |
| 530 ## - ADDITIONAL PHYSICAL FORM AVAILABLE NOTE | |
| Additional physical form available note | Issued also as CD |
| 653 #4 - INDEX TERM--UNCONTROLLED | |
| Uncontrolled term | Breast cancer |
| 653 #4 - INDEX TERM--UNCONTROLLED | |
| Uncontrolled term | Cyclin dependent kinase (CDK7) |
| 653 #4 - INDEX TERM--UNCONTROLLED | |
| Uncontrolled term | Estrogen Receptor (ER) |
| 700 0# - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Gabriel B. Lopez , |
| Relator term | |
| 700 0# - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Salama A. Salama , |
| Relator term | |
| 700 0# - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Samia A. Shouman , |
| Relator term | |
| 856 ## - ELECTRONIC LOCATION AND ACCESS | |
| Uniform Resource Identifier | <a href="http://172.23.153.220/th.pdf">http://172.23.153.220/th.pdf</a> |
| 905 ## - LOCAL DATA ELEMENT E, LDE (RLIN) | |
| Cataloger | Nazla |
| Reviser | Revisor |
| 905 ## - LOCAL DATA ELEMENT E, LDE (RLIN) | |
| Cataloger | Shimaa |
| Reviser | Cataloger |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) | |
| Source of classification or shelving scheme | Dewey Decimal Classification |
| Koha item type | Thesis |
| Source of classification or shelving scheme | Not for loan | Home library | Current library | Date acquired | Full call number | Barcode | Date last seen | Koha item type | Copy number |
|---|---|---|---|---|---|---|---|---|---|
| Dewey Decimal Classification | المكتبة المركزبة الجديدة - جامعة القاهرة | قاعة الرسائل الجامعية - الدور الاول | 11.02.2024 | Cai01.19.02.Ph.D.2020.Ya.T | 01010110081710000 | 22.09.2023 | Thesis | ||
| Dewey Decimal Classification | المكتبة المركزبة الجديدة - جامعة القاهرة | مخـــزن الرســائل الجـــامعية - البدروم | 11.02.2024 | Cai01.19.02.Ph.D.2020.Ya.T | 01020110081710000 | 22.09.2023 | CD - Rom | 81710.CD |