Immunological studies on salmonellae isolated from different sources / (Record no. 78362)
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| 000 -LEADER | |
|---|---|
| fixed length control field | 03224cam a2200349 a 4500 |
| 003 - CONTROL NUMBER IDENTIFIER | |
| control field | EG-GiCUC |
| 005 - DATE AND TIME OF LATEST TRANSACTION | |
| control field | 20250223032617.0 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION | |
| fixed length control field | 201020s2020 ua h f m 000 0 eng d |
| 040 ## - CATALOGING SOURCE | |
| Original cataloging agency | EG-GiCUC |
| Language of cataloging | eng |
| Transcribing agency | EG-GiCUC |
| 041 0# - LANGUAGE CODE | |
| Language code of text/sound track or separate title | eng |
| 049 ## - LOCAL HOLDINGS (OCLC) | |
| Holding library | Deposite |
| 097 ## - Thesis Degree | |
| Thesis Level | Ph.D |
| 099 ## - LOCAL FREE-TEXT CALL NUMBER (OCLC) | |
| Classification number | Cai01.10.10.Ph.D.2020.En.I |
| 100 0# - MAIN ENTRY--PERSONAL NAME | |
| Personal name | Enas Atef Shedeed Younis |
| 245 10 - TITLE STATEMENT | |
| Title | Immunological studies on salmonellae isolated from different sources / |
| Statement of responsibility, etc. | Enas Atef Shedeed Younis ; Supervised Jakeen Kamal Abdelhaleem Elgakee , Mahmoud Dardiri Elhariri , Soad Abdelaziz Abdelwanis |
| 246 15 - VARYING FORM OF TITLE | |
| Title proper/short title | دراسات مناعيه على السالمونيلا المعزولة من مصادر مختلفة |
| 260 ## - PUBLICATION, DISTRIBUTION, ETC. | |
| Place of publication, distribution, etc. | Cairo : |
| Name of publisher, distributor, etc. | Enas Atef Shedeed Younis , |
| Date of publication, distribution, etc. | 2020 |
| 300 ## - PHYSICAL DESCRIPTION | |
| Extent | 76 P. : |
| Other physical details | facsmilies ; |
| Dimensions | 25cm |
| 502 ## - DISSERTATION NOTE | |
| Dissertation note | Thesis (Ph.D.) - Cairo University - Faculty of Veterinary Medicine - Department of Microbiology |
| 520 ## - SUMMARY, ETC. | |
| Summary, etc. | Salmonella infection is a critical veterinary and medical problem world-wide and is a major issue in the food industry. Non-typhoidal Salmonella is known significantly as an important gastroenteritis-related pathogen. There is limited data specifically discussing the outer membrane proteins (OMPs), a distinguishable characteristic of Gram negative bacteria situated at host{u2013} bacterial interface and are significant for virulence, host immune responses and drug therapy targets. Enhanced diagnosis of live poultry colonized with Salmonella species is required to avoid food borne diseases. The widely available ELISA assays are currently based on O-antigens (LPS) mixture or Salmonella total cell lysate and are unorganized by cross-reaction. The present study is based on molecular characterization of OMPs among four Salmonella serovars (S.Typhimurium, S. Enteritidis, S. Kentucky and S. Anatum) using SDS-PAGE, the isolates were confirmed by culturing, biochemical, serological and molecular (real-time PCR targeting invA gene) identifications. The OMPs profiling showed more than 70 protein bands ranging in size from 208 kDa to below 16 kDa which were detected using Totallab 1D 12.2software. It is clear that all Salmonella strains had bands at 54-60 kDa, 45-53 kDa, 36-39 kDa and 26-31 kDa. Eleven strains had bands at 41-46 kDa and 33-35 kDa. Nine strains had bands at 61-69 kDa. Eight strains had bands at 135-145 kDa and 72-79 kDa. Seven strains had bands at 108-123 kDa and 83-91 kDa. In the Western blot analysis, the prepared hyper immune serum of each Salmonella serovars reacted with the outer membrane protein band 35kDa. The finding highlighted that the OMP 35kDa protein of studied salmonellae could be immune-response protein. An Indirect ELISA developed in this study holds promise as screening of poultry flocks for Salmonella infection |
| 530 ## - ADDITIONAL PHYSICAL FORM AVAILABLE NOTE | |
| Additional physical form available note | Issued also as CD |
| 653 #4 - INDEX TERM--UNCONTROLLED | |
| Uncontrolled term | ELISA |
| 653 #4 - INDEX TERM--UNCONTROLLED | |
| Uncontrolled term | Outer Membrane Proteins |
| 653 #4 - INDEX TERM--UNCONTROLLED | |
| Uncontrolled term | Salmonella |
| 700 0# - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Jakeen Kamal Abdelhaleem Elgakee , |
| Relator term | |
| 700 0# - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Mahmoud Dardiri Elhariri , |
| Relator term | |
| 700 0# - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Soad Abdelaziz Abdelwanis , |
| Relator term | |
| 856 ## - ELECTRONIC LOCATION AND ACCESS | |
| Uniform Resource Identifier | <a href="http://172.23.153.220/th.pdf">http://172.23.153.220/th.pdf</a> |
| 905 ## - LOCAL DATA ELEMENT E, LDE (RLIN) | |
| Cataloger | Amira |
| Reviser | Cataloger |
| 905 ## - LOCAL DATA ELEMENT E, LDE (RLIN) | |
| Cataloger | Nazla |
| Reviser | Revisor |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) | |
| Source of classification or shelving scheme | Dewey Decimal Classification |
| Koha item type | Thesis |
| Source of classification or shelving scheme | Not for loan | Home library | Current library | Date acquired | Full call number | Barcode | Date last seen | Koha item type | Copy number |
|---|---|---|---|---|---|---|---|---|---|
| Dewey Decimal Classification | المكتبة المركزبة الجديدة - جامعة القاهرة | قاعة الرسائل الجامعية - الدور الاول | 11.02.2024 | Cai01.10.10.Ph.D.2020.En.I | 01010110081808000 | 22.09.2023 | Thesis | ||
| Dewey Decimal Classification | المكتبة المركزبة الجديدة - جامعة القاهرة | مخـــزن الرســائل الجـــامعية - البدروم | 11.02.2024 | Cai01.10.10.Ph.D.2020.En.I | 01020110081808000 | 22.09.2023 | CD - Rom | 81808.CD |