Development of a real-time multiplex PCR assay for detection of Salmonellae in chicken samples / (Record no. 78645)
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| 000 -LEADER | |
|---|---|
| fixed length control field | 03231cam a2200349 a 4500 |
| 003 - CONTROL NUMBER IDENTIFIER | |
| control field | EG-GiCUC |
| 005 - DATE AND TIME OF LATEST TRANSACTION | |
| control field | 20250223032626.0 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION | |
| fixed length control field | 201109s2020 ua d f m 000 0 eng d |
| 040 ## - CATALOGING SOURCE | |
| Original cataloging agency | EG-GiCUC |
| Language of cataloging | eng |
| Transcribing agency | EG-GiCUC |
| 041 0# - LANGUAGE CODE | |
| Language code of text/sound track or separate title | eng |
| 049 ## - LOCAL HOLDINGS (OCLC) | |
| Holding library | Deposite |
| 097 ## - Thesis Degree | |
| Thesis Level | Ph.D |
| 099 ## - LOCAL FREE-TEXT CALL NUMBER (OCLC) | |
| Classification number | Cai01.10.10.Ph.D.2020.Am.D |
| 100 0# - MAIN ENTRY--PERSONAL NAME | |
| Personal name | Amany Abdellattif Mosleh Abdelfatah |
| 245 10 - TITLE STATEMENT | |
| Title | Development of a real-time multiplex PCR assay for detection of Salmonellae in chicken samples / |
| Statement of responsibility, etc. | Amany Abdellattif Mosleh Abdelfatah ; Supervised Jakeen Kamal Abdelhalem Eljakee , Sherif Abdelmonem Omar , Amany Ibrahim Elbialy |
| 246 15 - VARYING FORM OF TITLE | |
| Title proper/short title | تطويراختبار تفاعل البلمرة المتسلسل الوقتى الحقيقى متعدد للكشف عن السالمونيلا فى عينات الدجاج |
| 260 ## - PUBLICATION, DISTRIBUTION, ETC. | |
| Place of publication, distribution, etc. | Cairo : |
| Name of publisher, distributor, etc. | Amany Abdellattif Mosleh Abdelfatah , |
| Date of publication, distribution, etc. | 2020 |
| 300 ## - PHYSICAL DESCRIPTION | |
| Extent | 72 P. : |
| Other physical details | charts , facsimiles ; |
| Dimensions | 25cm |
| 502 ## - DISSERTATION NOTE | |
| Dissertation note | Thesis (Ph.D.) - Cairo University - Faculty of Veterinary Medicine - Department of Microbiology |
| 520 ## - SUMMARY, ETC. | |
| Summary, etc. | Salmonella is the pathogenic agent of salmonellosis, a major cause of enteric illness and typhoid fever. Salmonella Typhimurium infects poultry causing significant losses, serovar 1, 4, [5], 12: i: - is considered a monophasic variant of S. Typhimurium that increasingly important as public health risk. A total of 58 Salmonella isolates were reidentified by slide agglutination test according to White-Kauffmann- Le Minor scheme. A duplex syber green real time PCR was applied for detection of genus Salmonella and S. Typhimurium using 16S rRNA and fliCgenes. All strains harbor 16S rRNA. Twenty one strains harborfliC gene that including S. Typhimurium (12), S. Kentucky ( 6) , S.1,4,[5],12:i:- (1) , S. Lagos (1) and S. Kedougou (1). A duplex Tagman real time -PCR was performed for differentiation between biphasic S. Typhimurium and monophasic variant 1,4,[5],12:i:- using fljB1,2 and fliB / IS200 in the Fli A. B intergenic region. Ten strains out of 12 S. Typhimurium harbor fljB1,2 however strain S1,4,[5],12:i:- don't possess this gene. While 13 strains were positive to fliB / IS200(12 S. Typhimurium and S 1,4,[5],12:i:-). The fliB / IS200 is specific gene for S .Typhimurium (biphasic and monophasic).These results indicate that two strains serologically confirmed to be S. Typhimurium and S.1,4,[5],12:i:- don't possess fljB1,2 and have fliB/ IS200genes are monophasic variants bya duplex Tagman real time -PCR. It was noticed that prolonged subculture and or repeat phase inversion method leads to formation of flakes that in turn cause wrongly serotyping identification. Real time {u2013}PCR is rapid and can be used to identifying and differentiation between biphasic and monophasic S.1, 4, [5], 12: i: - S. Typhimurium |
| 530 ## - ADDITIONAL PHYSICAL FORM AVAILABLE NOTE | |
| Additional physical form available note | Issued also as CD |
| 653 #4 - INDEX TERM--UNCONTROLLED | |
| Uncontrolled term | Biphasic and monophasic S. |
| 653 #4 - INDEX TERM--UNCONTROLLED | |
| Uncontrolled term | FljB1,2 gene |
| 653 #4 - INDEX TERM--UNCONTROLLED | |
| Uncontrolled term | Typhimurium |
| 700 0# - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Amany Ibrahim Elbialy , |
| Relator term | |
| 700 0# - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Jakeen Kamal Abdelhalem Eljakee , |
| Relator term | |
| 700 0# - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Sherif Abdelmonem Omar , |
| Relator term | |
| 856 ## - ELECTRONIC LOCATION AND ACCESS | |
| Uniform Resource Identifier | <a href="http://172.23.153.220/th.pdf">http://172.23.153.220/th.pdf</a> |
| 905 ## - LOCAL DATA ELEMENT E, LDE (RLIN) | |
| Cataloger | Nazla |
| Reviser | Revisor |
| 905 ## - LOCAL DATA ELEMENT E, LDE (RLIN) | |
| Cataloger | Shimaa |
| Reviser | Cataloger |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) | |
| Source of classification or shelving scheme | Dewey Decimal Classification |
| Koha item type | Thesis |
| Source of classification or shelving scheme | Not for loan | Home library | Current library | Date acquired | Full call number | Barcode | Date last seen | Koha item type | Copy number |
|---|---|---|---|---|---|---|---|---|---|
| Dewey Decimal Classification | المكتبة المركزبة الجديدة - جامعة القاهرة | قاعة الرسائل الجامعية - الدور الاول | 11.02.2024 | Cai01.10.10.Ph.D.2020.Am.D | 01010110081958000 | 22.09.2023 | Thesis | ||
| Dewey Decimal Classification | المكتبة المركزبة الجديدة - جامعة القاهرة | مخـــزن الرســائل الجـــامعية - البدروم | 11.02.2024 | Cai01.10.10.Ph.D.2020.Am.D | 01020110081958000 | 22.09.2023 | CD - Rom | 81958.CD |