Characterization and functional analysis of an insecticidal protein from baculovirus / (رقم التسجيلة. 79396)
[ عرض عادي ]
| 000 -LEADER | |
|---|---|
| fixed length control field | 02880cam a2200349 a 4500 |
| 003 - CONTROL NUMBER IDENTIFIER | |
| control field | EG-GiCUC |
| 005 - DATE AND TIME OF LATEST TRANSACTION | |
| control field | 20250223032649.0 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION | |
| fixed length control field | 201231s2020 ua dho f m 000 0 eng d |
| 040 ## - CATALOGING SOURCE | |
| Original cataloging agency | EG-GiCUC |
| Language of cataloging | eng |
| Transcribing agency | EG-GiCUC |
| 041 0# - LANGUAGE CODE | |
| Language code of text/sound track or separate title | eng |
| 049 ## - LOCAL HOLDINGS (OCLC) | |
| Holding library | Deposite |
| 097 ## - Thesis Degree | |
| Thesis Level | M.Sc |
| 099 ## - LOCAL FREE-TEXT CALL NUMBER (OCLC) | |
| Classification number | Cai01.12.25.M.Sc.2020.No.C |
| 100 0# - MAIN ENTRY--PERSONAL NAME | |
| Personal name | Norhan Yasser Sayed Moaz |
| 245 10 - TITLE STATEMENT | |
| Title | Characterization and functional analysis of an insecticidal protein from baculovirus / |
| Statement of responsibility, etc. | Norhan Yasser Sayed Moaz ; Supervised Saeed Ahmed Soliman Ghozlan , Maha Mohamed Youssef Alkhazindar , Wael Hassan Ali Elmenofy |
| 246 15 - VARYING FORM OF TITLE | |
| Title proper/short title | التوصيف والتحليل الوظيفي لبروتين مبيد للحشرات من الفيروس العصوي |
| 260 ## - PUBLICATION, DISTRIBUTION, ETC. | |
| Place of publication, distribution, etc. | Cairo : |
| Name of publisher, distributor, etc. | Norhan Yasser Sayed Moaz , |
| Date of publication, distribution, etc. | 2020 |
| 300 ## - PHYSICAL DESCRIPTION | |
| Extent | 61 P . , (12) P. : |
| Other physical details | charts , facsmilies , photographs ; |
| Dimensions | 25cm |
| 502 ## - DISSERTATION NOTE | |
| Dissertation note | Thesis (M.Sc.) - Cairo University - Faculty of Science - Department of Biotechnology |
| 520 ## - SUMMARY, ETC. | |
| Summary, etc. | Chitinase plays a highly important function in the growth of insects and its development by hydrolyzing chitin in the body of the insect. In this study we have cloned, expressed and characterized chitinase gene A (ChiA) from the cotton leafworm, Spodoptera littoralis nucleopolyhedrovirus, an Egyptian isolate. The Spli-ChiA gene shown to have an open reading frame of 1809 nucleotides, encoding a protein of 599 amino acid residues with a predicted weight of 66.8 kDa. The phylogenetic analysis revealed that the Spli-ChiA deduced protein sequence shared extensive similarities with other baculovirus chitinases to be found in the GenBank. The SDS-PAGE analysis of SpliNPV-infected larvae total protein followed by western blotting with anti-SpliNPV chitinase antibody identified a protein of about ~50 kDa corresponding to the mature chitinase of SpliNPV. Temporal transcriptional analyses using RT-PCR and qRT-PCR revealed that Spli-ChiA transcription might be detected in SpliNPV infect larvae at 12 hpi with a steady increase in the transcription levels up to 71.4-fold at 120 hpi, suggesting the late transcription of chitinase gene. In addition, the purified Spli-ChiA protein showed inhibition in vitro of the growth of two fungi species, Fusarium solani and Fusarium oxysporum, confirming the expressed protein activity. The results concluded that SpliNPV chitinase (Spli-ChiA) is a good applicant protein for significant contribution in pest control |
| 530 ## - ADDITIONAL PHYSICAL FORM AVAILABLE NOTE | |
| Additional physical form available note | Issued also as CD |
| 653 #4 - INDEX TERM--UNCONTROLLED | |
| Uncontrolled term | Chitinase gene A |
| 653 #4 - INDEX TERM--UNCONTROLLED | |
| Uncontrolled term | qRT-PCR |
| 653 #4 - INDEX TERM--UNCONTROLLED | |
| Uncontrolled term | Spodoptera littoralis NPV |
| 700 0# - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Maha Mohamed Youssef Alkhazindar , |
| Relator term | |
| 700 0# - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Said Ahmed Soliman Ghozlan , |
| Relator term | |
| 700 0# - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Wael Hassan Ali Elmenofy , |
| Relator term | |
| 856 ## - ELECTRONIC LOCATION AND ACCESS | |
| Uniform Resource Identifier | <a href="http://172.23.153.220/th.pdf">http://172.23.153.220/th.pdf</a> |
| 905 ## - LOCAL DATA ELEMENT E, LDE (RLIN) | |
| Cataloger | Amira |
| Reviser | Cataloger |
| 905 ## - LOCAL DATA ELEMENT E, LDE (RLIN) | |
| Cataloger | Nazla |
| Reviser | Revisor |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) | |
| Source of classification or shelving scheme | Dewey Decimal Classification |
| Koha item type | Thesis |
| Source of classification or shelving scheme | Not for loan | Home library | Current library | Date acquired | Full call number | Barcode | Date last seen | Koha item type | Copy number |
|---|---|---|---|---|---|---|---|---|---|
| Dewey Decimal Classification | المكتبة المركزبة الجديدة - جامعة القاهرة | قاعة الرسائل الجامعية - الدور الاول | 11.02.2024 | Cai01.12.25.M.Sc.2020.No.C | 01010110082455000 | 22.09.2023 | Thesis | ||
| Dewey Decimal Classification | المكتبة المركزبة الجديدة - جامعة القاهرة | مخـــزن الرســائل الجـــامعية - البدروم | 11.02.2024 | Cai01.12.25.M.Sc.2020.No.C | 01020110082455000 | 22.09.2023 | CD - Rom | 82455.CD |