Mutation patterns of Epidermal Growth Factor Receptor (EGFR) gene in Non-Small Cell Lung Cancer (NSCLC) of Egyptian patients / (Record no. 79465)

MARC details
000 -LEADER
fixed length control field 03220cam a2200349 a 4500
003 - CONTROL NUMBER IDENTIFIER
control field EG-GiCUC
005 - DATE AND TIME OF LATEST TRANSACTION
control field 20250223032651.0
008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION
fixed length control field 210104s2020 ua dh f m 000 0 eng d
040 ## - CATALOGING SOURCE
Original cataloging agency EG-GiCUC
Language of cataloging eng
Transcribing agency EG-GiCUC
041 0# - LANGUAGE CODE
Language code of text/sound track or separate title eng
049 ## - LOCAL HOLDINGS (OCLC)
Holding library Deposite
097 ## - Thesis Degree
Thesis Level M.Sc
099 ## - LOCAL FREE-TEXT CALL NUMBER (OCLC)
Classification number Cai01.12.21.M.Sc.2020.Mo.M
100 0# - MAIN ENTRY--PERSONAL NAME
Personal name Mona Mahmoud Qenawi Farrag
245 10 - TITLE STATEMENT
Title Mutation patterns of Epidermal Growth Factor Receptor (EGFR) gene in Non-Small Cell Lung Cancer (NSCLC) of Egyptian patients /
Statement of responsibility, etc. Mona Mahmoud Qenawi Farrag ; Supervised Salwa Farouk Sabet , Heba Bassiony Atwa , Wafaa Hassan Elmetnawy
246 15 - VARYING FORM OF TITLE
Title proper/short title أنماط الطفرات لجين مستقبلات عامل نمو البشرة فى سرطان الرئة ذى الخلايا غير الصغيرة للمرضى المصريين
260 ## - PUBLICATION, DISTRIBUTION, ETC.
Place of publication, distribution, etc. Cairo :
Name of publisher, distributor, etc. Mona Mahmoud Qenawi Farrag ,
Date of publication, distribution, etc. 2020
300 ## - PHYSICAL DESCRIPTION
Extent 101 P. :
Other physical details charts , facimiles ;
Dimensions 25cm
502 ## - DISSERTATION NOTE
Dissertation note Thesis (M.Sc.) - Cairo University - Faculty of Science - Department of Zoology
520 ## - SUMMARY, ETC.
Summary, etc. Background: Mutations of epidermal growth factor receptor (EGFR) gene have been reported to be linked with non{u2013}small cell lung cancer (NSCLC) and associated with the responsiveness of tumors to EGFR tyrosine kinase inhibitors. This study aimed to assess the frequency of EGFR mutations in Egyptian patients with NSCLC adenocarcinoma. Patients and Methods: Formalin-fixed paraffinembedded (FFPE) tissue blocks were prepared from 120 NSCLC patients and 20 healthy volunteers. DNA extracted from FFPE samples was used for PCR amplification using biotinylated primers. The amplified products were hybridized to a test strip containing allele-specific oligonucleotide probes immobilized as an array of parallel lines using the Vienna Lab Strip Assays kit and EGFR mutations were identified. Results: EGFR mutations were detected in forty nine (40.8%) patients, whereas 71 (59.1%) patients were non-mutant. Moreover, the most common mutations were found in exon 19 (55.1% of the all mutations) and exon 21 (26.5% of the all mutations), whereas the mutations in the other exons were less common; mutations in exons 18 and 20 represented only 10.2 % and 8.2% respectively. We found that exon 21 mutation L858R (Leu858Arg) represents 22.4% of mutations and L747-P753 mutation in Ex19Del represents 18.4% of mutations which are the most common. On the other hand, no EGFR mutations were detected in the healthy individuals. Conclusion: EGFR mutations were determined in exons 18 to 21, where exons 19 and 21 were the most frequent mutations and were related to non-smokers and women. We recommend that somatic EGFR mutations are valuable biomarkers for NSCLC diagnosis and can be used for developing targeted therapy in the Egyptian population
530 ## - ADDITIONAL PHYSICAL FORM AVAILABLE NOTE
Additional physical form available note Issued also as CD
653 #4 - INDEX TERM--UNCONTROLLED
Uncontrolled term Epidermal Growth Factor Receptor (EGFR)
653 #4 - INDEX TERM--UNCONTROLLED
Uncontrolled term Mutations
653 #4 - INDEX TERM--UNCONTROLLED
Uncontrolled term Non{u2013}Small Cell Lung Cancer
700 0# - ADDED ENTRY--PERSONAL NAME
Personal name Heba Bassiony Atwa ,
Relator term
700 0# - ADDED ENTRY--PERSONAL NAME
Personal name Salwa Farouk Sabet ,
Relator term
700 0# - ADDED ENTRY--PERSONAL NAME
Personal name Wafaa Hassan Elmetnawy ,
Relator term
856 ## - ELECTRONIC LOCATION AND ACCESS
Uniform Resource Identifier <a href="http://172.23.153.220/th.pdf">http://172.23.153.220/th.pdf</a>
905 ## - LOCAL DATA ELEMENT E, LDE (RLIN)
Cataloger Nazla
Reviser Revisor
905 ## - LOCAL DATA ELEMENT E, LDE (RLIN)
Cataloger Shimaa
Reviser Cataloger
942 ## - ADDED ENTRY ELEMENTS (KOHA)
Source of classification or shelving scheme Dewey Decimal Classification
Koha item type Thesis
Holdings
Source of classification or shelving scheme Not for loan Home library Current library Date acquired Full call number Barcode Date last seen Koha item type Copy number
Dewey Decimal Classification   المكتبة المركزبة الجديدة - جامعة القاهرة قاعة الرسائل الجامعية - الدور الاول 11.02.2024 Cai01.12.21.M.Sc.2020.Mo.M 01010110082510000 22.09.2023 Thesis  
Dewey Decimal Classification   المكتبة المركزبة الجديدة - جامعة القاهرة مخـــزن الرســائل الجـــامعية - البدروم 11.02.2024 Cai01.12.21.M.Sc.2020.Mo.M 01020110082510000 22.09.2023 CD - Rom 82510.CD
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