DNA analysis of challenging forensic samples models toward fully integrated short tandem repeats / (Record no. 80373)
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| 000 -LEADER | |
|---|---|
| fixed length control field | 03056cam a2200349 a 4500 |
| 003 - CONTROL NUMBER IDENTIFIER | |
| control field | EG-GiCUC |
| 005 - DATE AND TIME OF LATEST TRANSACTION | |
| control field | 20250223032720.0 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION | |
| fixed length control field | 210327s2021 ua dh f m 000 0 eng d |
| 040 ## - CATALOGING SOURCE | |
| Original cataloging agency | EG-GiCUC |
| Language of cataloging | eng |
| Transcribing agency | EG-GiCUC |
| 041 0# - LANGUAGE CODE | |
| Language code of text/sound track or separate title | eng |
| 049 ## - LOCAL HOLDINGS (OCLC) | |
| Holding library | Deposite |
| 097 ## - Thesis Degree | |
| Thesis Level | M.Sc |
| 099 ## - LOCAL FREE-TEXT CALL NUMBER (OCLC) | |
| Classification number | Cai01.12.21.M.Sc.2021.Sh.D |
| 100 0# - MAIN ENTRY--PERSONAL NAME | |
| Personal name | Shereen Saleh Mahmoud Elliethy |
| 245 10 - TITLE STATEMENT | |
| Title | DNA analysis of challenging forensic samples models toward fully integrated short tandem repeats / |
| Statement of responsibility, etc. | Shereen Saleh Mahmoud Elliethy ; Supervised M. Akmal Elghorr , Haidan Mostafa Elshorbagy , Mona Elkotb Moussa Sharaf |
| 246 15 - VARYING FORM OF TITLE | |
| Title proper/short title | تحليل الدنا لنموذج لعينات الطب الشرعى الصعبة بهدف التوثيق المتكامل للتكرارات القصيرة الترادفية |
| 260 ## - PUBLICATION, DISTRIBUTION, ETC. | |
| Place of publication, distribution, etc. | Cairo : |
| Name of publisher, distributor, etc. | Shereen Saleh Mahmoud Elliethy , |
| Date of publication, distribution, etc. | 2021 |
| 300 ## - PHYSICAL DESCRIPTION | |
| Extent | 76 P. : |
| Other physical details | charts , facsimiles ; |
| Dimensions | 25cm |
| 502 ## - DISSERTATION NOTE | |
| Dissertation note | Thesis (M.Sc.) - Cairo University - Faculty of Science - Department of Zoology |
| 520 ## - SUMMARY, ETC. | |
| Summary, etc. | Most of the forensic genetic analytical methods used today employ short tandem repeats (STRs) to provide discriminatory power. The data showed that different factors may affect STR profiles in challenging samples including DNA quantity, DNA quality, PCR inhibitors and storage time. In the present study, dried bloodstained samples on two types of stained fabrics (black cotton and denim), contain challenging dyes, have been used to compare the efficiency of two different DNA-extraction methods (automated magnetic beads based method (EZ1) , and the organic method), with and without using carrier RNA molecules, to assess the quality and quantity of the extracted DNA, and their capabilities for producing reportable STR-profiles, at two storage time.Carrier RNA was added following cell lysis, that caused a dramatically increase in DNA recoveries on the EZ1 in contrast to organic method. EZ1 was found to be preferred than organic especially when a time passed over. Organic method preferred when samples are available in small quantities. In addition, using carrier RNA during the organic method steps showed no improvement in STR profiling. EZ1 with carrier RNA was preferred for bloodstain samples on fabrics with textile dyes (black dye or denim indigo), especially at long storage. Denim was more problematic than Cotton due to presence of challenging inhibitors (indigo dye) which lead us to use EZ1 method other than using organic method with inhibitory components like phenol. A better understanding of these inhibitory samples could lead to the development of more sensitive, more robust analytical protocols |
| 530 ## - ADDITIONAL PHYSICAL FORM AVAILABLE NOTE | |
| Additional physical form available note | Issued also as CD |
| 653 #4 - INDEX TERM--UNCONTROLLED | |
| Uncontrolled term | EZ1 |
| 653 #4 - INDEX TERM--UNCONTROLLED | |
| Uncontrolled term | Organic method |
| 653 #4 - INDEX TERM--UNCONTROLLED | |
| Uncontrolled term | STR profile |
| 700 0# - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Haidan Mostafa Elshorbagy , |
| Relator term | |
| 700 0# - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Mohamed Akmal Elghorr , |
| Relator term | |
| 700 0# - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Mona Elkotb Moussa Sharaf , |
| Relator term | |
| 856 ## - ELECTRONIC LOCATION AND ACCESS | |
| Uniform Resource Identifier | <a href="http://172.23.153.220/th.pdf">http://172.23.153.220/th.pdf</a> |
| 905 ## - LOCAL DATA ELEMENT E, LDE (RLIN) | |
| Cataloger | Nazla |
| Reviser | Revisor |
| 905 ## - LOCAL DATA ELEMENT E, LDE (RLIN) | |
| Cataloger | Shimaa |
| Reviser | Cataloger |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) | |
| Source of classification or shelving scheme | Dewey Decimal Classification |
| Koha item type | Thesis |
| Source of classification or shelving scheme | Not for loan | Home library | Current library | Date acquired | Full call number | Barcode | Date last seen | Koha item type | Copy number |
|---|---|---|---|---|---|---|---|---|---|
| Dewey Decimal Classification | المكتبة المركزبة الجديدة - جامعة القاهرة | قاعة الرسائل الجامعية - الدور الاول | 11.02.2024 | Cai01.12.21.M.Sc.2021.Sh.D | 01010110083025000 | 22.09.2023 | Thesis | ||
| Dewey Decimal Classification | المكتبة المركزبة الجديدة - جامعة القاهرة | مخـــزن الرســائل الجـــامعية - البدروم | 11.02.2024 | Cai01.12.21.M.Sc.2021.Sh.D | 01020110083025000 | 22.09.2023 | CD - Rom | 83025.CD |