Biochemical and immune studieson nano-antibody / (Record no. 81433)
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| 000 -LEADER | |
|---|---|
| fixed length control field | 03102cam a2200337 a 4500 |
| 003 - CONTROL NUMBER IDENTIFIER | |
| control field | EG-GiCUC |
| 005 - DATE AND TIME OF LATEST TRANSACTION | |
| control field | 20250223032754.0 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION | |
| fixed length control field | 210629s2021 ua d f m 000 0 eng d |
| 040 ## - CATALOGING SOURCE | |
| Original cataloging agency | EG-GiCUC |
| Language of cataloging | eng |
| Transcribing agency | EG-GiCUC |
| 041 0# - LANGUAGE CODE | |
| Language code of text/sound track or separate title | eng |
| 049 ## - LOCAL HOLDINGS (OCLC) | |
| Holding library | Deposite |
| 097 ## - Thesis Degree | |
| Thesis Level | Ph.D |
| 099 ## - LOCAL FREE-TEXT CALL NUMBER (OCLC) | |
| Classification number | Cai01.07.04.Ph.D.2021.Ba.B |
| 100 0# - MAIN ENTRY--PERSONAL NAME | |
| Personal name | Basem Sayed Abbas Ahmed |
| 245 10 - TITLE STATEMENT | |
| Title | Biochemical and immune studieson nano-antibody / |
| Statement of responsibility, etc. | Basem Sayed Abbas Ahmed ; Supervised Emam Abdelmobdy Abdelrahim , Amr Ahmed Moustafa |
| 246 15 - VARYING FORM OF TITLE | |
| Title proper/short title | دراسات كيميائية حيوية ومناعية على الاجسام المضادة متناهية الصغر |
| 260 ## - PUBLICATION, DISTRIBUTION, ETC. | |
| Place of publication, distribution, etc. | Cairo : |
| Name of publisher, distributor, etc. | Basem Sayed Abbas Ahmed , |
| Date of publication, distribution, etc. | 2021 |
| 300 ## - PHYSICAL DESCRIPTION | |
| Extent | 180 P. : |
| Other physical details | charts , facsimiles ; |
| Dimensions | 25cm |
| 502 ## - DISSERTATION NOTE | |
| Dissertation note | Thesis (Ph.D.) - Cairo University - Faculty of Agriculture - Department of Agriculture Biochemistry |
| 520 ## - SUMMARY, ETC. | |
| Summary, etc. | Recently, the spread of epidemics has increased in conjunction with the ability of microbes to develop themselves and increase their ability to resist antimicrobial, which forced scientists to search for unconventional methods of treatment and quick and sensitive methods of diagnosis.In this research the study was done using camel to produce different Nano-antibodies those have the ability to diagnose and treat different types of pathogenic bacteria (Mannheimiahaemolytica, Salmonellatyphimurium, Salmonellaarizonae, and Klebsiellapneumoniae), which characterized by cost, skills and production requirements are lower than the traditional antibodies.The present work developed a sandwich ELISA-based Nano- antibody towards Mannheimia haemolytica,Salmonella typhimurium, Salmonella arizonae, and Klebsiella pneumoniae as the first report for the treatment of them. VHH amplified fragments at 450 bp were isolated and cloned into the phagemid vector pMECS, then via phage display technique isolate the specific Nano-antibody against each strain, then screening by ELISA to obtain the top 5 AD value Nano- antibody for each strain. Nano-antibodies protein was purified under non-denaturing conditions. The purifiedNano-antibodies were chractraized and used for Competed ELISA, it is show binding to the specific strain, and also, there was a cross-reaction with the other strains. Nano-antibodies are expected to survive in harsh conditions, such as those found in the stomach gut, creating opportunities for nano-antibodies' oral delivery to treat different diseases as gastrointestinal diseases. But these Nano- antibodies cannot use in diagnostic. So, many trials should be done to improve its sensitivity in the future, and can use the epitops that characterizes each strain as antigen to immunizing the camel to produce the nano antibody used for diagnostic |
| 530 ## - ADDITIONAL PHYSICAL FORM AVAILABLE NOTE | |
| Additional physical form available note | Issued also as CD |
| 653 #4 - INDEX TERM--UNCONTROLLED | |
| Uncontrolled term | Diagnostic |
| 653 #4 - INDEX TERM--UNCONTROLLED | |
| Uncontrolled term | Nano-antibody |
| 653 #4 - INDEX TERM--UNCONTROLLED | |
| Uncontrolled term | VHH |
| 700 0# - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Amr Ahmed Moustafa , |
| Relator term | |
| 700 0# - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Emam Abdelmobdy Abdelrahim , |
| Relator term | |
| 856 ## - ELECTRONIC LOCATION AND ACCESS | |
| Uniform Resource Identifier | <a href="http://172.23.153.220/th.pdf">http://172.23.153.220/th.pdf</a> |
| 905 ## - LOCAL DATA ELEMENT E, LDE (RLIN) | |
| Cataloger | Nazla |
| Reviser | Revisor |
| 905 ## - LOCAL DATA ELEMENT E, LDE (RLIN) | |
| Cataloger | Shimaa |
| Reviser | Cataloger |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) | |
| Source of classification or shelving scheme | Dewey Decimal Classification |
| Koha item type | Thesis |
| Source of classification or shelving scheme | Not for loan | Home library | Current library | Date acquired | Full call number | Barcode | Date last seen | Koha item type | Copy number |
|---|---|---|---|---|---|---|---|---|---|
| Dewey Decimal Classification | المكتبة المركزبة الجديدة - جامعة القاهرة | قاعة الرسائل الجامعية - الدور الاول | 11.02.2024 | Cai01.07.04.Ph.D.2021.Ba.B | 01010110083769000 | 22.09.2023 | Thesis | ||
| Dewey Decimal Classification | المكتبة المركزبة الجديدة - جامعة القاهرة | مخـــزن الرســائل الجـــامعية - البدروم | 11.02.2024 | Cai01.07.04.Ph.D.2021.Ba.B | 01020110083769000 | 22.09.2023 | CD - Rom | 83769.CD |