Studies on gene regulation of some genes that related to bt. toxins resistance in the cotton worm, spodoptera littoralis / (Record no. 81525)

MARC details
000 -LEADER
fixed length control field 02867cam a2200349 a 4500
003 - CONTROL NUMBER IDENTIFIER
control field EG-GiCUC
005 - DATE AND TIME OF LATEST TRANSACTION
control field 20250223032757.0
008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION
fixed length control field 210731s2021 ua dh f m 000 0 eng d
040 ## - CATALOGING SOURCE
Original cataloging agency EG-GiCUC
Language of cataloging eng
Transcribing agency EG-GiCUC
041 0# - LANGUAGE CODE
Language code of text/sound track or separate title eng
049 ## - LOCAL HOLDINGS (OCLC)
Holding library Deposite
097 ## - Thesis Degree
Thesis Level M.Sc
099 ## - LOCAL FREE-TEXT CALL NUMBER (OCLC)
Classification number Cai01.07.10.M.Sc.2021.Ha.S
100 0# - MAIN ENTRY--PERSONAL NAME
Personal name Hager Mohsen Hassan Ali Khalil
245 10 - TITLE STATEMENT
Title Studies on gene regulation of some genes that related to bt. toxins resistance in the cotton worm, spodoptera littoralis /
Statement of responsibility, etc. Hager Mohsen Hassan Ali Khalil ; Supervised Nagwa Ibrahim Abdelfattah Elarabi , Dalia Sayed Ahmed Hasaneen , Saad Mohamed Moussa
246 15 - VARYING FORM OF TITLE
Title proper/short title Spodoptera littoralisفى دودة ورق القطن (Bt. Toxins) دراسات حول التنظيم الجينى لبعض الجينات التى لها علاقة بالمقاومة ضد المبيدات
260 ## - PUBLICATION, DISTRIBUTION, ETC.
Place of publication, distribution, etc. Cairo :
Name of publisher, distributor, etc. Hager Mohsen Hassan Ali Khalil ,
Date of publication, distribution, etc. 2021
300 ## - PHYSICAL DESCRIPTION
Extent 93 P. :
Other physical details charts , facimiles ;
Dimensions 25cm
502 ## - DISSERTATION NOTE
Dissertation note Thesis (M.Sc.) - Cairo University - Faculty of Agriculture - Department of Genetics
520 ## - SUMMARY, ETC.
Summary, etc. A better understanding of the processing of Bacillus thuringiensis (Bt) Cry1C toxin in the midgut of Spodoptera littoralis larvae is very important to characterize the main regulatory elements of Bt tolerance. The current study aimed to evaluate the mRNA level of trypsin (Try), aminopeptidase N (APN), alkaline phosphatase (ALP), cadherin (Cad), and cytochrome P450 (CYP) in both susceptible and Cry1C- tolerant strains of S. littoralis. Total RNA was extracted from susceptible and tolerant strains to construct cDNA. Quantitative real-time PCR (qPCR) showed a significant upregulation of CYP gene in tolerant strain. In contrast, the levels of expression of Try, ALP and Cad were significantly downregulated in tolerant strain. APN relative mRNA expression did not show significant differences between susceptible and tolerant strains. Histologically, the midgut of late third-instar larvae of tolerant population S. littoralis showed vacuolization of the epithelium and disruption of both the peritrophic membrane and the striated boarder compared to the susceptible strain. Our data indicate the important roles of CYP, Try, ALP and Cad in the resistance development and toxicity to Bt Cry1C.The obtained results are useful for further illustrating of Bt Cry1C processing and S. littoralis tolerance
530 ## - ADDITIONAL PHYSICAL FORM AVAILABLE NOTE
Additional physical form available note Issued also as CD
653 #4 - INDEX TERM--UNCONTROLLED
Uncontrolled term Bacillus thuringiensis
653 #4 - INDEX TERM--UNCONTROLLED
Uncontrolled term Bt Cry1C toxin
653 #4 - INDEX TERM--UNCONTROLLED
Uncontrolled term Spodoptera littoralis
700 0# - ADDED ENTRY--PERSONAL NAME
Personal name Dalia Sayed Ahmed Hasaneen ,
Relator term
700 0# - ADDED ENTRY--PERSONAL NAME
Personal name Nagwa Ibrahim Abdelfattah Elarabi ,
Relator term
700 0# - ADDED ENTRY--PERSONAL NAME
Personal name Saad Mohamed Moussa ,
Relator term
856 ## - ELECTRONIC LOCATION AND ACCESS
Uniform Resource Identifier <a href="http://172.23.153.220/th.pdf">http://172.23.153.220/th.pdf</a>
905 ## - LOCAL DATA ELEMENT E, LDE (RLIN)
Cataloger Nazla
Reviser Revisor
905 ## - LOCAL DATA ELEMENT E, LDE (RLIN)
Cataloger Shimaa
Reviser Cataloger
942 ## - ADDED ENTRY ELEMENTS (KOHA)
Source of classification or shelving scheme Dewey Decimal Classification
Koha item type Thesis
Holdings
Source of classification or shelving scheme Not for loan Home library Current library Date acquired Full call number Barcode Date last seen Koha item type Copy number
Dewey Decimal Classification   المكتبة المركزبة الجديدة - جامعة القاهرة قاعة الرسائل الجامعية - الدور الاول 11.02.2024 Cai01.07.10.M.Sc.2021.Ha.S 01010110083799000 22.09.2023 Thesis  
Dewey Decimal Classification   المكتبة المركزبة الجديدة - جامعة القاهرة مخـــزن الرســائل الجـــامعية - البدروم 11.02.2024 Cai01.07.10.M.Sc.2021.Ha.S 01020110083799000 22.09.2023 CD - Rom 83799.CD
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