Chemical studies on molecular detection and nanobiocidal control of some enterobacteriaceae foodborne bacteria / (رقم التسجيلة. 82134)

تفاصيل مارك
000 -LEADER
fixed length control field 03869cam a2200349 a 4500
003 - CONTROL NUMBER IDENTIFIER
control field EG-GiCUC
005 - DATE AND TIME OF LATEST TRANSACTION
control field 20250223032815.0
008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION
fixed length control field 210914s2021 ua dh f m 000 0 eng d
040 ## - CATALOGING SOURCE
Original cataloging agency EG-GiCUC
Language of cataloging eng
Transcribing agency EG-GiCUC
041 0# - LANGUAGE CODE
Language code of text/sound track or separate title eng
049 ## - LOCAL HOLDINGS (OCLC)
Holding library Deposite
097 ## - Thesis Degree
Thesis Level Ph.D
099 ## - LOCAL FREE-TEXT CALL NUMBER (OCLC)
Classification number Cai01.12.02.Ph.D.2021.He.C
100 0# - MAIN ENTRY--PERSONAL NAME
Personal name Heba Allah Mohamed Mohamed Nour Eldin
245 10 - TITLE STATEMENT
Title Chemical studies on molecular detection and nanobiocidal control of some enterobacteriaceae foodborne bacteria /
Statement of responsibility, etc. Heba Allah Mohamed Mohamed Nour Eldin ; Supervised Mahfouz Ali Abdelaziz , Nashwa Mohamed Helmy , Kamla Mohamed Elsaied
246 15 - VARYING FORM OF TITLE
Title proper/short title دراسات كيميائية على الكشف الجزيئى والتحكم باستخدام مواد النانو الحيوية لبعض البكتيريا المعوية المنقولة عن طريق الأغذية
260 ## - PUBLICATION, DISTRIBUTION, ETC.
Place of publication, distribution, etc. Cairo :
Name of publisher, distributor, etc. Heba Allah Mohamed Mohamed Nour Eldin ,
Date of publication, distribution, etc. 2021
300 ## - PHYSICAL DESCRIPTION
Extent 170 P. :
Other physical details charts , facsimiles ;
Dimensions 25cm
502 ## - DISSERTATION NOTE
Dissertation note Thesis (Ph.D.) - Cairo University - Faculty of Science - Department of Biochemistry
520 ## - SUMMARY, ETC.
Summary, etc. Foodborne diseases are caused by consumption of food spoiled by pathogens or their toxins. Sampling beef meat and chicken samples from local markets which is additionally affecting foodborne disease outbreaks, making food safety a universal issue. Food safety nowadays, remains focused on prevention and monitoring of potentially harmful effects growingly correlated with a number of different microbial subtypes and sub strains that may produce toxic products not easily detectable by standard methods Targeted molecular profiling of Enterobactericea in randomly collected samples is traced with their health risk impact on human. Biochemical classical microbiological, real time PCR, MALDI TOF as well as sequencing confirmation techniques were performed for speciation of possible foodborne pathogens may present in meat and tissue samples. Salmonella, E-coli are confirmed, 6 samples were selected for further confirmation by sequencing. A study on nanobiocidal control investigations were tried on some Enenterobactericea species using newly prepared nanoparticles.Curcumin chitosan nanocomposite and selenium nanoparticles were synthesized by ionotropic gelation and green methods respectively.The biosynthesized nanoparticles were characterized by using Fourier transform spectroscopy (FT- IR), Dynamic Light Scattering (DLS), Zeta Potential (ZP) and Transmission Electron Microscopy (TEM). Antibacterial activities of Curcumin Chitosan nanocomposites with size of 160nm and surface charge of +24mv and selenium nanoparticles of 30-50nm and -22mv were tested against Escherichia coli O157:H7 and Salmonella species by broth macrodilution method and Minimum inhibitory concentration (MIC) of bacterial growth was determined. Curcumin Chitosan nanocomposite inhibit bacterial growth at lower concentration compared to selenium nanoparticles which may referred to high solubility of chitosan with curcumin rather than bare selenium nanoparticles.Results indicate that Curcumin Chitosan nanocomposite distort and damage bacterial cell membrane, resulting in a leakage of intracellular contents and eventually the death of bacterial cells as detected by TEM on broth media 24hrs post incubation with standard strains of E coli O157:H7 and Salmonella specie.Minimum inhibitory concentration (MIC) of bacterial growth was determined
530 ## - ADDITIONAL PHYSICAL FORM AVAILABLE NOTE
Additional physical form available note Issued also as CD
653 #4 - INDEX TERM--UNCONTROLLED
Uncontrolled term Enenterobactericea
653 #4 - INDEX TERM--UNCONTROLLED
Uncontrolled term MALDI-TOF
653 #4 - INDEX TERM--UNCONTROLLED
Uncontrolled term Sequencing
700 0# - ADDED ENTRY--PERSONAL NAME
Personal name Kamla Mohamed Elsaied ,
Relator term
700 0# - ADDED ENTRY--PERSONAL NAME
Personal name Mahfouz Ali Abdelaziz ,
Relator term
700 0# - ADDED ENTRY--PERSONAL NAME
Personal name Nashwa Mohamed Helmy ,
Relator term
856 ## - ELECTRONIC LOCATION AND ACCESS
Uniform Resource Identifier <a href="http://172.23.153.220/th.pdf">http://172.23.153.220/th.pdf</a>
905 ## - LOCAL DATA ELEMENT E, LDE (RLIN)
Cataloger Nazla
Reviser Revisor
905 ## - LOCAL DATA ELEMENT E, LDE (RLIN)
Cataloger Shimaa
Reviser Cataloger
942 ## - ADDED ENTRY ELEMENTS (KOHA)
Source of classification or shelving scheme Dewey Decimal Classification
Koha item type Thesis
المقتنيات
Source of classification or shelving scheme Not for loan Home library Current library Date acquired Full call number Barcode Date last seen Koha item type Copy number
Dewey Decimal Classification   المكتبة المركزبة الجديدة - جامعة القاهرة قاعة الرسائل الجامعية - الدور الاول 11.02.2024 Cai01.12.02.Ph.D.2021.He.C 01010110084189000 22.09.2023 Thesis  
Dewey Decimal Classification   المكتبة المركزبة الجديدة - جامعة القاهرة مخـــزن الرســائل الجـــامعية - البدروم 11.02.2024 Cai01.12.02.Ph.D.2021.He.C 01020110084189000 22.09.2023 CD - Rom 84189.CD
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