Thesis (M.Sc.) - Cairo University - Faculty of Veterinary Medicine - Department of Microbiology

Randomly 100 natural reared Tilapia samples were collected from river nile tributaries, (Elharania, Aysh Sakarah, Elharam street, Gezeret bein Elbahreen, Gezeret Elkorsa, Elotobis Elnahry Moneeb), Giza Egypt. Some examined fish showed erosion of tail and fin (Tail and fin rot) heamorrhages of body parts. The target organs for isolation was liver, kidney and blood morphological and biochemical identification of bacterial isolates by using VITEK 2 (BIOMERIEUX) and BiOLOG (MicroStation{u2122} System/MicroLog): revealed Enterococcus faecium, Enterococcus casseliflavus, Staphylococcus warneri, S. lentus, S. arlettea, S. xylosus, S. heamolyticus, S. gallinarum, pseudomonas luteola, pseudomonas putida, Aeromonas bio var sobria and aeromonas hydrophila. Histopathologically collected samples were liver, kidney and spleen. Congestion of central vein and hepatic sinusoids and showing fatty change of hepatocytes, fusion of gill lamellae associated with inflammatory cells infiltration showing focal necrosis of gill, spleen showing hemorrhage and haemosiderosis and focal melanin carrying cells for Water sample examination for heavy metal pollution (Cadmium - Lead - Cupper - Zinc) for oxidative STRESS RESPONSE TEST for fish samples (liver) to determination of malondialdehyde, superoxide dismutase, Glutathione peroxidase, and reduced glutathione there is significant increases in all enzyme and that proved heavy metals pollution. And for DNA fragmentation assay there was fragmented

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