Purification and characterization of laccase from some soil microorganisms / Mohamed Naguib Mohamed Abdelghany ; Supervsied Mohamed Ibrahim Ahmed Ali , Salama Abuoelyazeed Ouf

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Mohamed Naguib Mohamed Abdelghany

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TextLanguage: English Publication details: Cairo : Mohamed Naguib Mohamed Abdelghany , 2016Description: 177 P. : charts , facsimiles ; 25cmOther title:

تنقية و توصيف انزيم اللاكاز من بعض الكائنات الدقيقة الموجودة بالتربة [Added title page title]

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Dissertation note: Thesis (Ph.D.) - Cairo University - Faculty of Science - Department of Botany and Microbiology Summary: In this work, the microfloral picture from Saint Catherine protectorate (SCP), Giza zoo garden (GZG) and Cairo university (CU) soils was studied. Twenty five fungal isolates, six bacterial isolates were isolated. Aspergillus flavus isolated from saint Catherine protectorate (SCP) was the most active organism in laccase production. The culture medium was optimized for laccase production. Laccase was further purified and characterized, it showed an apparent molecular weight of 68.5 kDa by SDS-PAGE. Aspergillus flavus purified laccase was capable of decolorizing seven dyes of concentration with different degrees, the highest and most rapid decolorization occurs with malachite green. Laccase tested on malachite green decolorization with coexistence of metal ions and organic solvents and was also tested on real textile effluent over 6 days. Aspergillus flavus was deposited in NCBI Gen Bank and was given a strain identifier, A. flavus NG85. Aspergillus flavus NG85 putative laccase gene was isolated, sequenced, with accession number KM522917.2

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Item type	Current library	Home library	Call number	Copy number	Status	Barcode
Thesis	قاعة الرسائل الجامعية - الدور الاول	المكتبة المركزبة الجديدة - جامعة القاهرة	Cai01.12.05.Ph.D.2016.Mo.P (Browse shelf(Opens below))		Not for loan	01010110071481000
CD - Rom	مخـــزن الرســائل الجـــامعية - البدروم	المكتبة المركزبة الجديدة - جامعة القاهرة	Cai01.12.05.Ph.D.2016.Mo.P (Browse shelf(Opens below))	71481.CD	Not for loan	01020110071481000

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Thesis (Ph.D.) - Cairo University - Faculty of Science - Department of Botany and Microbiology

In this work, the microfloral picture from Saint Catherine protectorate (SCP), Giza zoo garden (GZG) and Cairo university (CU) soils was studied. Twenty five fungal isolates, six bacterial isolates were isolated. Aspergillus flavus isolated from saint Catherine protectorate (SCP) was the most active organism in laccase production. The culture medium was optimized for laccase production. Laccase was further purified and characterized, it showed an apparent molecular weight of 68.5 kDa by SDS-PAGE. Aspergillus flavus purified laccase was capable of decolorizing seven dyes of concentration with different degrees, the highest and most rapid decolorization occurs with malachite green. Laccase tested on malachite green decolorization with coexistence of metal ions and organic solvents and was also tested on real textile effluent over 6 days. Aspergillus flavus was deposited in NCBI Gen Bank and was given a strain identifier, A. flavus NG85. Aspergillus flavus NG85 putative laccase gene was isolated, sequenced, with accession number KM522917.2

Issued also as CD

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