Thesis (Ph.D.) - Cairo University - Faculty of Veterinary Medicine - Department of Microbiology

Snuffle disease isone of the most important health problems in rabbits. It is caused by P. multocida.A total of 116 New-Zealand rabbits were used in evaluationof four prepared polyvalent P. multocida (serotypes A: 1, A: 3, A :12 and D: 2) vaccines.First vaccine wasformalized non adjuvanted Pasteurella vaccine (FV). Second one was Lipid A adjuvanted Pasteurella vaccine (AV) in which the Lipid A was self-prepared, extracted from E. coli O: 157 and evaluated by High Performance Liquid Chromatography(HPLC). Third one was Montanide TM ISA 70 VG adjuvanted Pasteurella vaccine (MV). Fourth one was Montanide TM IMS1313 VG N PR adjuvanted Pasteurella vaccine (NV) and finally control groups. Comparisons between the prepared vaccines were done by challenge test, Lysozyme activity test, IHA test and ELISA. Statistical analysis was done. MontanideTM ISA 70 VG adjuvanted Pasteurella vaccine and Lipid A adjuvanted Pasteurella vaccines were the best, followed by Formalized non adjuvanted Pasteurella vaccine then Montanide TM IMS1313 VG N PR adjuvanted Pasteurella vaccine

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