Thesis (M.Sc.) - Cairo University - Faculty of Science - Department of Botany and Microbiology

This study aimed to focus on the environmental surface contamination with pathogenic organisms contribute to the development of health care-associated infect ions (HAI) and evaluate effect of bee venom from Apis mellifera as the antibacterial activity against the isolated strains. 50 samples from commonly touched surfaces in intensive care units (ICUs) in two different hospitals were collected. Al l pathogenic samples were identified biochemically and assigned to their genera and species using RT- PCR technique. The bee venom was analyzed spectrophotometrically and gave the chemical formula C₃₁ H₂₂₈ N₃₈O₃₂ which suggests melittin as active component in the bee venom then tested against the bacteria which showed antibacterial effect on all the tested microorganisms and the minimum inhibitory concentration was 50.36, 39.22 and 62.5 og/ml on E.coli, A.baumannii and P. aureginosa. And by studying the hemolytic effect it showed that under the venom concentration of 150og/ml. no hemolysis was observed

Issued also as CD