Molecular studies on oocyte maturation in egyptian buffalo using different media / Said Elhussein Said Mahmoud Abuhamed ; Supervised Salah Eldin Sayed Mohamed Elassal , Ahmed Yehia Gad , Swiefy Abdelrahim Swiefy
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TextLanguage: English Publication details: Cairo : Said Elhussein Said Mahmoud Abuhamed , 2019Description: 97 P. : charts , facsimiles ; 25cmOther title: - دراسات جزيئية على انضاج بويضات الجاموس المصرى باستخدام بيئات مختلفة [Added title page title]
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Thesis
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قاعة الرسائل الجامعية - الدور الاول | المكتبة المركزبة الجديدة - جامعة القاهرة | Cai01.07.10.M.Sc.2019.Sa.M (Browse shelf(Opens below)) | Not for loan | 01010110079083000 | ||
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مخـــزن الرســائل الجـــامعية - البدروم | المكتبة المركزبة الجديدة - جامعة القاهرة | Cai01.07.10.M.Sc.2019.Sa.M (Browse shelf(Opens below)) | 79083.CD | Not for loan | 01020110079083000 |
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| Cai01.07.10.M.Sc.2019.Ne.S Studying some genes affecting milk productivity in egyptian buffaloes / | Cai01.07.10.M.Sc.2019.Ne.S Studying some genes affecting milk productivity in egyptian buffaloes / | Cai01.07.10.M.Sc.2019.Sa.M Molecular studies on oocyte maturation in egyptian buffalo using different media / | Cai01.07.10.M.Sc.2019.Sa.M Molecular studies on oocyte maturation in egyptian buffalo using different media / | Cai01.07.10.M.Sc.2020.Es.M Molecular genetics studies of resistance of some plants to nematodes / | Cai01.07.10.M.Sc.2020.Es.M Molecular genetics studies of resistance of some plants to nematodes / | Cai01.07.10.M.Sc.2021.Ge.S Studies on agrobacterium-mediated genetic transformation of rice using dreb2a gene / |
Thesis (M.Sc.) - Cairo University - Faculty of Agriculture - Department of Genetics
Retinoic acid, vitamin A metabolite, plays a role in oocyte development and maturation in different ways including gene expression alteration and/or prohibiting oxidative stress. The objective of this study was to examine the effect of 9-cis-retinoic acid (9-cisRA) on the quality and maturation rate of buffalo oocytes. Cumulusoocyte complexes (COCs, n = 460) were collected from ovaries of slaughtered buffalos. Varying concentrations of 9-cisRA (0, 5, 50, and 200 nM) were added to the maturation medium, and the following parameters were analyzed: (i) maturation and cleavage rates, (ii) mitochondrial activity and reactive oxygen species (ROS) levels, (iii) expression level of antioxidant-related genes (PRDX1, SOD1, CAT, HOMX1, and GPX4) using RT-qPCR. (iv) expression level of genes related to oocyte quality (GDF9,BMP15) Maturation rate was significantly improved in 5 nM 9-cisRA oocyte group (95.8%, P < .05) compared to control and other treatment groups (86.7% in control group). The same oocyte group exhibited significantly higher mitochondrial membrane potential activity and lower ROS accumulation level compared to other treatment groups. Antioxidant-related genes were up-regulated in oocytes matured with 5 or 50 nM 9-cisRA compared to control and 200 nM 9-cisRA groups. In contrast, 200 nM of 9-cisRA showed a clear down-regulation for antioxidant-related genes except for PRDX1
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