Thesis (Ph.D.) - Cairo University - Faculty of African Postgraduate Studies - Department of Natural Resources

This study was carried out at the Natural Resources Department, Faculty of African Postgraduate Studies, Cairo University, Giza, Egypt. And Fruit, Ornamental plants, Woody Trees Department, Horticulture Research Institute, Giza, Egypt; during the successive seasons of 2015-2018. The aim of this work is to improve micropropagation conditions of Simmondsia chinensis using micro-cuttings in order to explore the possibility of inducing mutation through in vitro technique and the production of salinity tolerant mutants. Sterilized jojoba micro-cuttings were cultured on {u00BE} MS starting medium free of growth regulators. Regenerated shoots were exposed to 0.0, 10, 15, 20 and 25 Kr doses of gamma ray. Or treated with 0.0, 1%, 2%, 3% and 4% concentrations of EMS. Multiplication MS medium contained different concentrations of BA alone or with Kin. Afterwards, to evaluate the reaction of the resulting mutagenized tissues to salinity stress; Regenerated shoots were treated with sea salt concentrations at 0.0, 15%, 25%, 50% and 100.0%. Results indicated that the highest number of shoots (12 shoot/ explant) was obtained using 3.0 g/l BA in medium. While, combination of 3.0 mg/l BA and 0.5 mg/l Kin resulted in high shoot formation and number of shoots of (9.50 shoot/ explant). Results showed that jojoba shoots irradiated with 20 kr gamma ray and treated with 25% and 50% sea salt resulted in survival percentage of (80% and 60%) respectively in comparison with control (93.33%)

Issued also as CD