Thesis (Ph.D.) - Cairo University - Faculty of Agriculture - Department of Agronomy

Five lentil cultivars were used in this study. Three of which belonged to microsperma seed types (Giza 9, Giza 29 and Giza 370) and two ones are considered as macrosperma (Sinai 1 and Giza 51). Dry seeds of five Egyptian lentil cultivars (M₀) were irradiated by two Helium neon laser types and gamma irradiation (Cobalt 60) with three exposure periods in addition to untreated corresponding seeds. The exposure periods were 5, 10 and 15 min of both laser types, whereas gamma rays were applied on 2, 4 and 6 min which equal 3, 6 and 9 kr. The M₁ plants along to untreated corresponding checks were evaluated under pot condition during 2014/2015 season using 30 cm plastic pots, each sown with five healthy seed and represent one replicate. The performance of M₂ and M3 high yielding selected individuals and bulk represent lasers and gamma exposure period combinations with control checks were investigated under pot trial during 2015/2016 and 2016/2017 seasons, respectively. Morphology, yield and its components of segregated mutations compared to untreated checks M₂ seeds were studied using protein profiling (SDS-PAGE). The M3 pot grown plants of higher doses and exposure periods were sampled for molecular characterization. RAPD and ISSR techniques were used for molecular studied

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