صورة الغلاف المحلية
صورة الغلاف المحلية
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Isolation, molecular characterization and expression of nitrite reductase (NiR) gene responsible for the absorption of some air pollutants / Warda Alaa Eldin Mahmoud Hashim ; Supervised Said Abdrahman Salih , Rania Mahmoud Abou Ali

بواسطة: المساهم: نوع المادة : نصاللغة: الإنجليزية تفاصيل النشر: Cairo : Warda Alaa Eldin Mahmoud Hashim , 2020الوصف: 86 P. : charts , facsimiles ; 25cmعنوان آخر:
  • عزل و توصيف جزيئى والتعبير عن جين مختزل النيتريت المسؤول عن امتصاص بعض ملوثات الهواء [عنوان مضاف عنوان الصفحة]
الموضوع: موارد على الإنترنت: Available additional physical forms:
  • Issued also as CD
ملاحظة الأطروحة: Thesis (M.Sc.) - Cairo University - Faculty of Science - Department of Biotechnology ملخص: A nitrite reductase (NiR) gene was isolated, cloned and sequenced from nitrate induced leaves of the Egyptian Spinacia oleracea seedlings. The sequence of the isolated NiR gene has 1788 bp open reading frame. The NiR sequence was submitted into GenBank under accession number MH729808. The deduced amino acid sequence is 595 a.a, it has 96% homology with predicted ferredoxin-nitrite reductase, chloroplastic (Spinacia oleracea) and highly conserved. Leucine was the highest amino acid percentage in the predicted NiR protein, which has a higher preference for the Ü-helix. RT-PCR analysis showed that the expression level of NiR was the highest at 2 hr of nitrate treatment. The NiR protein had high activity towards NaNO2 substrate after 3 hr induction with potassium nitrate
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المقتنيات
نوع المادة المكتبة الحالية المكتبة الرئيسية رقم الاستدعاء رقم النسخة حالة الباركود
Thesis قاعة الرسائل الجامعية - الدور الاول المكتبة المركزبة الجديدة - جامعة القاهرة Cai01.12.25.M.Sc.2020.Wa.I (استعراض الرف(يفتح أدناه)) لا تعار 01010110080864000
CD - Rom مخـــزن الرســائل الجـــامعية - البدروم المكتبة المركزبة الجديدة - جامعة القاهرة Cai01.12.25.M.Sc.2020.Wa.I (استعراض الرف(يفتح أدناه)) 80864.CD لا تعار 01020110080864000

Thesis (M.Sc.) - Cairo University - Faculty of Science - Department of Biotechnology

A nitrite reductase (NiR) gene was isolated, cloned and sequenced from nitrate induced leaves of the Egyptian Spinacia oleracea seedlings. The sequence of the isolated NiR gene has 1788 bp open reading frame. The NiR sequence was submitted into GenBank under accession number MH729808. The deduced amino acid sequence is 595 a.a, it has 96% homology with predicted ferredoxin-nitrite reductase, chloroplastic (Spinacia oleracea) and highly conserved. Leucine was the highest amino acid percentage in the predicted NiR protein, which has a higher preference for the Ü-helix. RT-PCR analysis showed that the expression level of NiR was the highest at 2 hr of nitrate treatment. The NiR protein had high activity towards NaNO2 substrate after 3 hr induction with potassium nitrate

Issued also as CD

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