Thesis (M.Sc.) - Cairo University - Faculty of Oral and Dental Medicine - Department of Operative Dentistry

Forty-four carious lesions in Forty-four participants (one in each participant) were chosen in this study. The lesions were classified using the International Caries Detection and Assessment System (ICDAS-II). The teeth of the patients that meetinclusion criteria were anesthetized and isolated with a rubber dam. Cavity opened using conventional high-speed rotary instruments. The central cariogenic biomass and the superficial parts of the necrotic dentin were removed with round burs.The excavation procedure was terminated as soon as the soft and wet dentine was removed and the remaining tissue was leathery but not hard on exploring. A dentinal sample was then collected from the base of the cavity using sterile spoon excavator as a baseline for bacteriological assessment. After application of either intervention or control agent, another dentinal sample was collected using a sterile excavator and transferred into sterile tubes containing 1.5ml thioglycollate medium used as a carrier. This sterile tube was kept in an ice box and taken to the microbiology laboratory for processing, by another examiner who is blinded to the type of dentin surface treatment.The number of colonies were expressed as colony{u2013} forming units (CFU/ml) compared before and after application of the antibacterial agent.Finally the cavity outline was adjusted with burs; any undermined enamel around the cavity walls was removed with a small round bur. The teeth restored with resin composite according to the manufacturer's instructions

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