Thesis (Ph.D.) - Cairo University - Faculty of Medicine - Department of Ophthalmology

Background: The use of autologous fat grafting is limited by its unpredictable survival rate. In our study we aim at studying the effect of stem cell viability, harvested from the abdomen and thigh, on autologous fat grafts{u2019} survival in the orbital for treating the enophthalmic anophthalmic socket using CT volumetry. Methods: A randomized prospective interventional study including 24 patients suffering from unilateral enophthalmic anophthalmic socket. Pre-operative clinical assessment included photographs, exophthalmometry reading as well as CT volumetry for volume deficit calculations. The harvesting site was randomly allocated between patients (abdomen or thigh). Fat was sent to the laboratory to assess stem cell viabilityPatients were followed up to 6 months post-operatively. Percentage of fat retained 1 and 2 (Percentage of fat remaining at the last follow up compared to pre-operative measurements of the enophthalmic socket with the globe and without the globe respectively) was calculated as well as exophthalmometry change. Results: Mean percentage of retained fat 1 and 2 were 14.75% & 25.31% respectively. No statistically significant correlation was found between the sex, age of the patients, donor site, percentage of fat retained 1 and 2 or exophthalmometer change and the percentage of stem cell viability. Difficulty of extraction and degree of atrophia correlated significantly with percentage of fat retained 2 and not 1. Exophthalmometer change correlated significantly with percentage of fat retained 1 and not 2

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