TY - BOOK AU - Fatma El-Zahraa Samir Sobhy, AU - Sherif Helmy Ahmedm AU - Rania Saber Yousef AU - Shreif Said Saleh Ahmed TI - Biochemical studies of caper bush capparis spinosa l. by in vitro culture U1 - 632.95 PY - 2025/// KW - Agricultural Biochemistry KW - الكيمياء الحيوية الزراعية KW - Caper KW - Anti cancer activity KW - Tissue culture KW - Flavonoids KW - Secondary metabolites KW - Molecular docking KW - النشاط المضاد للسرطان KW - الكباريس N1 - Thesis (M.Sc)-Cairo University, 2025; Bibliography: pages 87-103; Issues also as CD N2 - Capparis spinosa L., an edible medicinal plant, is rich in flavonoids. Production of secondary metabolites preferentially uses amino acids as precursors using in vitro culture techniques. This study aimed to evaluate the effect of amino acids as elicitors on morphological characterization,non-antioxidant compounds accumilation, antioxidant enzymes, and anticancer activity Capparis spinosa was in vitro cultured on MS media containing three amino acids included; tryptophan, glutamine or phenylalanine at 100, 200, and 300 mg/L and compared with free amino acid culture (as control). After four weeks morphological characteristics were determined, some phytochemicals panel and rutin were evaluated by spectrophotometric and HPLC, respectively. The hepatocellular carcinoma cell line HepG2 was utilized to validate the anti cancer activity and in silico studies. The results showed that using colorex 15% resulted in 91.67% of disinfection.Applying 6-Benzylaminopurine(BAP) at 0.8 mg/L in MS media gave the highest shootlet length(4.03cm) ,but kinetin at 04 mg/L gave the highest shootlet number (6.11shoot/explant) . the most significant result (100%) to callus induction was achieved with MS medium supplemented with 0.5 mg/L 2,4-D for leaves.The MS media supplemented with 200 mg/L of tryptophan produced the most significant results, with an average shootlet number of 34.67. The explants grown on MS media supplemented with 100 mg/L of phenylalanine showed the greatest shootlet length (6.83 cm). Moreover, the explant grown on MS media with 200 mg/L of tryptophan had the largest number of leaves (16.32).Regarding fresh weight, the control group recorded the highest fresh plant weight 3.50 g, while the highest fresh weight of callus was 4.7 g under the treatment with tryptophan at 100 mg/L or glutamine at 300 mg/L. .The HPLC analysis of rutin for the callus treated with glutamine at 100 mg/L or phenylalanine 200 mg/L (the highest treatments of flavonoids) scored 88.2 and 64.3 µg/g, respectively. The toxicity of callus extract treated with glutamine at 100 mg/L was more toxic for cancer cells (HepG2) followed by phenylalanine at 200 mg/L at IC50 356.65 and 371.05 µg/mL, respectively. Additionally, in molecular docking, rutin effectively reduced the activity in the HepG2 promoter. It downregulated the mRNA expression of HepG2-responsive genes of liver cancer cells, reducing its capacity to form spheroids and blocking the HepG2 signaling pathway. These findings suggest that amino acid elicitors can improve the metabolism of Capparis spinosa L. and could be used to produce high amounts of rutin, which has antitumor activity against HepG2. Preventing oxidative damage and inducing apoptosis may be the mechanisms responsible for the anti-tumor effect. Rutin from Capparis spinosa presents a promising candidate for further in silico and in vitro anticancer research, particularly through molecular docking studies to explore its interactions with key oncogenic targets.; هدفت الدراسة إلى اختبار تأثير ثلاثة أحماض أمينية (تربتوفان، جلوتامين، فينيل ألانين) على نمو نبات الكباريس وإنتاج مركباته الثانوية ونشاطه المضاد للسرطان. أثبت محلول الكلوروكس 15% أعلى كفاءة تعقيم، بينما حقق BAP أعلى طول للساق والكينيتين أعلى عدد للأفرع. وأظهر التربتوفان 200 ملجم/لتر أفضل نمو من حيث عدد الأفرع، في حين أعطى الفينيل ألانين 100 ملجم/لتر أطول الأفرع. سجّل الجلوتامين 100 ملجم/لتر والفينيل ألانين 200 ملجم/لتر أعلى محتوى من الروتين. وأظهر الكالس المعالج بالجلوتامين 100 ملجم/لتر أقوى تأثير مضاد لخلايا سرطان الكبد HepG2. كما بيّن الربط الجزيئي قدرة الروتين على خفض التعبير الجيني للخلايا السرطانية وإيقاف مسار إشاراتها، مما يجعله مرشحًا واعدًا لأبحاث علاج السرطان ER -