05110namaa22004451i 4500 EG-GICUC 20260510132241.0 260510s2025 ua a|||frm||| 000 0 eng d EG-GICUC eng EG-GICUC EG-GICUC rda eng eng ara Deposit 660.6 660.6 21 M.Sc Cai01.07.06.M.Sc.2025.No.C Nora Rabee Gowda Abo El-kassem, preparation. Conservation of endangered medicinal plants natural resources through biotechnology techniques / by Nora Rabee Gowda Abo El-kassem ; Supervisors Dr. Sayed Abdel Kader Fayed, Dr. Mohamed AbdeL-Shakur, Dr. Mai Abdel - Moez Allam. حفظ الموارد الطبيعية للنباتات الطبية المعرضة للانقراض من خلال تقنيات التكنولوجيا الحيوية 2025. 110 pages : illustrations ; 25 cm. + CD. text rda content Unmediated rdamedia volume rdacarrier Thesis (Ph.D)-Cairo University, 2025. Bibliography: pages 89-110. The study investigated the effects of fourth different shoot induction media on growth. The first medium was BAv medium, a combination of 0.5 mg L− 1 benzyl adenine (BA), 5 mg L− 1 thiamine HCl, 0.5 mg L− 1 nicotinic acid, and 0.5 mg L− 1 pyridoxine was added to the initial medium. The second medium was MD medium supplemented with 3 mg L− 1 BA, 0.2 mg L− 1 naphthaleneacetic acid (NAA), 10 mg L− 1 thiamine HCl, 1 mg L− 1 nicotinic acid, and 1 mg L− 1 pyridoxine. The third medium was ABv medium formulated with 1 mg L− 1 Adenine sulfate, 1 mg L− 1 benzyl adenine, 10 mg L− 1 thiamine HCl, 1 mg L− 1 nicotinic acid, and 1 mg L− 1 pyridoxine. The fourth medium AS1v contains 4.4 g L-¹ MS, 30 g L-¹ sucrose, 1 mg L-¹ adenine sulfate, 5 mg L-¹ thiamine HCl, 0.5 mg L-¹ nicotinic acid, 0.5 mg L-¹ pyridoxine, and 2.8 g L-¹ gelrite. and three media for rooting, the first medium was IB medium supplemented with 0.4 mg L− 1 indol butyric acid, 5 mg L− 1 thiamine HCl, 0.5 mg L− 1 nicotinic acid, and 0.5 mg L− 1 pyridoxine. The second medium was IA medium containing 5 mg L− 1 thiamine HCl, 0.5 mg L− 1 nicotinic acid, and 0.5 mg L− 1 pyridoxine. The third medium was NA media formulated with 0.4 mg L− 1 naphthaleneacetic acid, 5 mg L− 1 thiamine HCl, 0.5 mg L− 1 nicotinic acid, and 0.5 mg L− 1 pyridoxine. All media were prepared using Murashige and Skoog (MS) basal salts 4.4 g L− 1 supplemented with 30 g L− 1 sucrose, and solidified with 2.8 g L− 1 gelrite. Various propagation protocols were tested based on the availability of plant materials. HPLC analysis revealed that the ethanolic extract had the highest quantities of rutin (19.07 µg/mL) and vanillin (6.29 µg/mL), while the aqueous extract had the highest levels of gallic acid (7.02 µg/mL) and chlorogenic acid (6.02 µg/mL). SDS-PAGE examination revealed five protein bands ranging in molecular weight from 28 kDa to 240 kDa. DNA barcoding was used for molecular authentication, and the acquired sequence was matched to GenBank database sequences using the BLAST tool. هدفت الدراسة إلى تقييم تأثير ثلاثة أوساط غذائية مختلفة على استحثاث الأفرع .وقد جرى اختبار عدة بروتوكولات إكثار وفقًا لتوافر المواد النباتية.أظهر تحليل HPLC أن المستخلص الإيثانولي احتوى على أعلى تركيز من الروتين (19.07 ميكروجرام/مل) والفانيلين (6.29 ميكروجرام/مل)، بينما سجل المستخلص المائي أعلى نسب من حمض الجاليك (7.02 ميكروجرام/مل) وحمض الكلوروجينيك (6.02 ميكروجرام/مل). كما أوضحت نتائج SDS-PAGE وجود خمسة نطاقات بروتينية تراوحت أوزانها الجزيئية بين 240 إلى 28 كيلودالتون. ولغرض التوثيق الجزيئي تم استخدام DNA barcoding، حيث تمت مقارنة التسلسل الناتج مع قاعدة بيانات GenBank باستخدام أداة BLAST Issues also as CD. Text in English and abstract in Arabic & English. Biotechnology Techniques تقنيات التكنولوجيا الحيوية Medicinal plants Endangered plants DNA barcoding SDS HPLC Sayed Abdel Kader Fayed thesis advisor. Mohamed AbdeL-Shakur thesis advisor. Mai Abdel - Moez Allam thesis advisor. 01-01-2025. Sayed Abdel Kader Fayed Mohamed AbdeL-Shakur Mai Abdel - Moez Allam Cairo University Faculty of Agriculture Department of Biochemistry Shimaa ddc TH 21 0 180075 ddc 317952 CUCL cl210 2026-05-10 93996 Cai01.07.06.M.Sc.2025.No.C 0101011093996000 2026-05-10 12:28:46 2026-05-10 TH