Experiment studies on transgenosis in chickens /
دراسات تجريبية على نقل الجينات فى الدجاج
Marwa Mostafa Mohamed Ahmed ; Supervised Essam Abbas Elgendy , Shoukry Mohamed Eltantawy , Shawky Ahmed Mohamed
- Cairo : Marwa Mostafa Mohamed Ahmed , 2012
- 71P. : facsimiles ; 25cm
Thesis (M.Sc.) - Cairo University - Faculty of Agriculture - Department of Animal Production
An experiment was carried out to develop transgenic chickens through the sperm mediated gene transfer (SMGT) technique using the plasmid DNA pUC18 as a target exogene ,Chickens of two lines (CE2 and CE4) were used . Seven treatments of semen were designed and included the incubation of sperm with the plasmid with a mixture of the plasmid and lipofection at 5% or 2.5% concentration and the incubation of spermatozoa with lipofectin and a semen extender (BPSE)