Rehab Ali Mohammed Mohammed Elhelw <br/><br/>
Production of monoclonal antibodies-based ELISA kits for pathogenic Leptospira spp. /  
 إنتاج مجموعات إليزا علي أساس الأجسام المضادة أحادية الفسيلة لليبتوسبيرا الممرضة 
Rehab Ali Mohammed Mohammed Elhelw ; Supervised Mahmoud Essam Hatem Ahmed , Mona Ibrahim Elnbaawy , Ahmed Abdelghani Elsanousi 
 - Cairo :   Rehab Ali Mohammed Mohammed Elhelw ,   2014 
 - 184 P. :   facsimiles ;   25cm 
<br/><br/>Thesis (Ph.D.) - Cairo University - Faculty of Veterinary Medicine - Department of Microbiology  
<br/><br/> A panel of monoclonal antibodies (MAbs) against each of Leptospira interrogans serovar Canicola and pathogenic Leptospira spp. were produced. L. interrogans serovar Canicola was cultivated, concentrated and prepared for fusion. BALB/c mice of 6 weeks old were inoculated with different antigenic preparations of L. interrogans serovar Canicola with complete freunds adjuvant. Booster doses with and without incomplete freunds adjuvant were applied. To obtain hybridomas, mice spleen cells were fused with myeloma cells in the presence of polyethylene glycol. Hybridoma cells that secret specific antibodies were selected on the basis of ELISA. These positive hybridomas were cloned by limiting dilution method to reveal subclones of one cell origin. 5 specific clones were obtained 
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<br/><br/><label>Subjects--Index Terms: </label>MAbs Pathogenic Leptospira spp.  Serovar Canicola